Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Joshua P Ferreira"'
Publikováno v:
PLoS ONE, Vol 9, Iss 11, p e113232 (2014)
The CRISPR (clustered regularly interspaced short palindromic repeats) platform has been developed as a general method to direct proteins of interest to gene targets. While the native CRISPR system delivers a nuclease that cleaves and potentially mut
Externí odkaz:
https://doaj.org/article/4a306a4b6fb243cc9a598a2e0f19e391
Autor:
Laura D. Attardi, Clifford L. Wang, Joshua P. Ferreira, D Kenzelmann Broz, S J Tapscott, Sabrina L. Spencer, K W Overton, William L Noderer, Tobias Meyer, Z J P Yang
Publikováno v:
Cell Death & Differentiation. 22:560-573
Acute muscle injury and physiological stress from chronic muscle diseases and aging lead to impairment of skeletal muscle function. This raises the question of whether p53, a cellular stress sensor, regulates muscle tissue repair under stress conditi
Publikováno v:
Bioengineered. 5:186-192
We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others
Autor:
Clifford L. Wang, Joshua P. Ferreira
Publikováno v:
Biotechnology Journal. 8:1476-1484
Although functional roles have been assigned to many genes, e.g. those involved in cell-cycle regulation, growth signaling, or cancer, considerably less is known about the quantitative relationship between gene expression levels and outcome. We devis
Publikováno v:
Integrative Biology. 4:108-114
We sought to characterize and compare wild-type and oncogenic Ras over-expression. Because different levels of Ras over-expression can have different effects on cell phenotype, it was important to evaluate a wide range of expression. Different expres
Publikováno v:
ACS Nano. 5:10026-10032
Single-walled carbon nanotubes (SWNTs) have shown promise for use in organic electronic applications including thin film transistors, conducting electrodes, and biosensors. Additionally, previous studies found applications for SWNTs in bioelectronic
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 110(28)
We engineered short ORFs and used them to control the expression level of recombinant proteins. These short ORFs, encoding a two-amino acid peptide, were placed upstream of an ORF encoding a protein of interest. Insertion of these upstream ORFs (uORF
The human cytomegalovirus and elongation factor 1α promoters are constitutive promoters commonly employed by mammalian expression vectors. These promoters generally produce high levels of expression in many types of cells and tissues. To generate a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0051380119e95e940b9cb6f977b84cb4
https://europepmc.org/articles/PMC3234317/
https://europepmc.org/articles/PMC3234317/