Zobrazeno 1 - 10
of 58
pro vyhledávání: '"Joshua D. Wythe"'
Autor:
Taeyong Ahn, Gabrielle E. Largoza, Julia Younis, Mary E. Dickinson, Chih-Wei Hsu, Joshua D. Wythe
Publikováno v:
STAR Protocols, Vol 5, Iss 2, Pp 103053- (2024)
Summary: Tissue clearing is an essential prerequisite for 3D volumetric imaging of larger tissues or organs. Here, we present a detailed protocol for optical, aqueous-based clearing of adult murine tissues using EZ Clear. We describe steps to ensure
Externí odkaz:
https://doaj.org/article/9322ef5749124732a8462233fa4d451f
Autor:
Ashley R. Ricciardelli, Ariadna Robledo, Jason E. Fish, Peter T. Kan, Tajie H. Harris, Joshua D. Wythe
Publikováno v:
Biomedicines, Vol 11, Iss 11, p 2876 (2023)
Brain arteriovenous malformations (bAVMs) are focal vascular lesions composed of abnormal vascular channels without an intervening capillary network. As a result, high-pressure arterial blood shunts directly into the venous outflow system. These high
Externí odkaz:
https://doaj.org/article/5985cf6a8cae4f38adf4124f37d75884
Autor:
Alexander M. Herman, Alexander M. Rhyner, W. Patrick Devine, Sean P. Marrelli, Benoit G. Bruneau, Joshua D. Wythe
Publikováno v:
Biology Open, Vol 7, Iss 3 (2018)
Canonical Notch signaling requires the presence of a membrane bound ligand and a corresponding transmembrane Notch receptor. Receptor engagement induces multiple proteolytic cleavage events culminating in the nuclear accumulation of the Notch intrace
Externí odkaz:
https://doaj.org/article/3ede507323064417bd724c3218b56e79
Autor:
Joshua D. Wythe, Michael J. Jurynec, Lisa D. Urness, Christopher A. Jones, M. Khaled Sabeh, Andreas A. Werdich, Mariko Sato, H. Joseph Yost, David J. Grunwald, Calum A. MacRae, Dean Y. Li
Publikováno v:
Disease Models & Mechanisms, Vol 4, Iss 5, Pp 607-621 (2011)
SUMMARY The vertebrate heart is one of the first organs to form, and its early function and morphogenesis are crucial for continued embryonic development. Here we analyze the effects of loss of Heart adaptor protein 1 (Hadp1), which we show is requir
Externí odkaz:
https://doaj.org/article/70b4d00401de4ceda2f37e882d663460
Autor:
Gab Seok Kim, Elisabeth Harmon, Andrea Doan, Alexander Andersohn, Ting Wu, Manuel C Gutierrez, Joshua D Wythe, Sean P Marrelli
Publikováno v:
Stroke. 54
Microglial cells (MG) serve as resident immune cells in the brain and play a critical role in the acute response and chronic recovery to stroke. We compared the MG transcriptomic response to stroke in young and aged mice by single-cell RNA sequencing
Autor:
David Mayerich, Joshua D. Wythe
Publikováno v:
Nature Cardiovascular Research. 1:691-693
Autor:
David Rohde, Katrien Vandoorne, I-Hsiu Lee, Jana Grune, Shuang Zhang, Cameron S. McAlpine, Maximilian J. Schloss, Ribhu Nayar, Gabriel Courties, Vanessa Frodermann, Gregory Wojtkiewicz, Lisa Honold, Qi Chen, Stephen Schmidt, Yoshiko Iwamoto, Yuan Sun, Sebastian Cremer, Friedrich F. Hoyer, Oriol Iborra-Egea, Christian Muñoz-Guijosa, Fei Ji, Bin Zhou, Ralf H. Adams, Joshua D. Wythe, Juan Hidalgo, Hideto Watanabe, Yookyung Jung, Anja M. van der Laan, Jan J. Piek, Youmna Kfoury, Pauline A. Désogère, Claudio Vinegoni, Partha Dutta, Ruslan I. Sadreyev, Peter Caravan, Antoni Bayes-Genis, Peter Libby, David T. Scadden, Charles P. Lin, Kamila Naxerova, Filip K. Swirski, Matthias Nahrendorf
Publikováno v:
Nature cardiovascular research, 1(1), 28-44
Abnormal hematopoiesis advances cardiovascular disease by generating excess inflammatory leukocytes that attack the arteries and the heart. The bone marrow niche regulates hematopoietic stem cell proliferation and hence the systemic leukocyte pool, b
Autor:
Chih-Wei Hsu, Juan Cerda, Jason M. Kirk, Williamson D. Turner, Tara L. Rasmussen, Carlos P. Flores Suarez, Mary E. Dickinson, Joshua D. Wythe
Publikováno v:
eLife. 11
Tissue clearing for whole organ cell profiling has revolutionized biology and imaging for exploration of organs in three-dimensional space without compromising tissue architecture. But complicated, laborious procedures, or expensive equipment, as wel
Autor:
Chih-Wei Hsu, Juan Cerda, Jason M Kirk, Williamson D Turner, Tara L Rasmussen, Carlos P Flores Suarez, Mary E Dickinson, Joshua D Wythe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b5e17d7b75d224aaa967e29446172375
https://doi.org/10.7554/elife.77419.sa2
https://doi.org/10.7554/elife.77419.sa2
Autor:
Joshua D. Wythe, Sameer S. Bajikar, Harini P. Tirumala, Yingyao Shao, Manuel Cantu Gutierrez, Huda Y. Zoghbi
Publikováno v:
Genes & Development. 35:489-494
While changes in MeCP2 dosage cause Rett syndrome (RTT) and MECP2 duplication syndrome (MDS), its transcriptional regulation is poorly understood. Here, we identified six putative noncoding regulatory elements of Mecp2, two of which are conserved in