Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Josh Tycko"'
Autor:
Georgi K. Marinov, Samuel H. Kim, S. Tansu Bagdatli, Soon Il Higashino, Alexandro E. Trevino, Josh Tycko, Tong Wu, Lacramioara Bintu, Michael C. Bassik, Chuan He, Anshul Kundaje, William J. Greenleaf
Publikováno v:
Genome Biology, Vol 24, Iss 1, Pp 1-15 (2023)
Abstract Detecting and mitigating off-target activity is critical to the practical application of CRISPR-mediated genome and epigenome editing. While numerous methods have been developed to map Cas9 binding specificity genome-wide, they are generally
Externí odkaz:
https://doaj.org/article/2a29028e5573430ebf0d2f915bbb3450
Autor:
Richard E Grewelle, Javier Perez-Saez, Josh Tycko, Erica K O Namigai, Chloe G Rickards, Giulio A De Leo
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 16, Iss 10, p e0010894 (2022)
CRISPR gene drives could revolutionize the control of infectious diseases by accelerating the spread of engineered traits that limit parasite transmission in wild populations. Gene drive technology in mollusks has received little attention despite th
Externí odkaz:
https://doaj.org/article/746d07ea006b47c799ea29d3d29ee6e0
Autor:
Josh Tycko, Michael Wainberg, Georgi K. Marinov, Oana Ursu, Gaelen T. Hess, Braeden K. Ego, Aradhana, Amy Li, Alisa Truong, Alexandro E. Trevino, Kaitlyn Spees, David Yao, Irene M. Kaplow, Peyton G. Greenside, David W. Morgens, Douglas H. Phanstiel, Michael P. Snyder, Lacramioara Bintu, William J. Greenleaf, Anshul Kundaje, Michael C. Bassik
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
Off-target effects in CRISPR screens for essential regulatory elements have not been systematically evaluated. Here the authors find Cas9 nuclease, CRISPRi/a each have distinct off-target effects, and that these can be accurately identified and remov
Externí odkaz:
https://doaj.org/article/fc5611c303ab470e9fff314f00d1301c
Autor:
Theresa Maier, Nicolas James Wheeler, Erica K O Namigai, Josh Tycko, Richard Ernest Grewelle, Yimtubezinash Woldeamanuel, Katharina Klohe, Javier Perez-Saez, Susanne H Sokolow, Giulio A De Leo, Timothy P Yoshino, Mostafa Zamanian, Jutta Reinhard-Rupp
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 13, Iss 12, p e0007833 (2019)
Schistosomiasis is one of the most important and widespread neglected tropical diseases (NTD), with over 200 million people infected in more than 70 countries; the disease has nearly 800 million people at risk in endemic areas. Although mass drug adm
Externí odkaz:
https://doaj.org/article/50b5116c38fc4ae1a0acda71a9c575f3
Autor:
Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, Jonathan S. Gootenberg, Omar O. Abudayyeh, Vic E. Myer, Christopher J. Wilson, Patrick D. Hsu
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-7 (2018)
A rigorous understanding of off-target effects is necessary for SaCas9 to be used in therapeutic genome editing. Here the authors measure SaCas9 mismatch tolerance across a pairwise library screen of 88,000 guides and targets in human cells and devel
Externí odkaz:
https://doaj.org/article/685c8caed9c1480ca04b435e72570dc1
Autor:
Josh Tycko, Luis A. Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, Jonathan S. Gootenberg, Omar O. Abudayyeh, Vic E. Myer, Christopher J. Wilson, Patrick D. Hsu
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-1 (2018)
The original HTML version of this Article incorrectly listed an affiliation of Josh Tycko as ‘Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA’, instead of the correct ‘Present address: Department of Genet
Externí odkaz:
https://doaj.org/article/fde8da885a3a48659bda8b8bf4e1a6f3
Autor:
Josh Tycko, Mike V. Van, null Aradhana, Nicole DelRosso, David Yao, Xiaoshu Xu, Connor Ludwig, Kaitlyn Spees, Katherine Liu, Gaelen T Hess, Mingxin Gu, Adi Xiyal Mukund, Peter H. Suzuki, Roarke A. Kamber, Lei S. Qi, Lacramioara Bintu, Michael C. Bassik
Human nuclear proteins contain >1000 transcriptional effector domains that can activate or repress transcription of target genes. We lack a systematic understanding of which effector domains regulate transcription robustly across genomic, cell-type,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8262fb71e21225ffdb58e41a337b2483
https://doi.org/10.1101/2023.05.12.540558
https://doi.org/10.1101/2023.05.12.540558
Autor:
Matthew G. Durrant, Alison Fanton, Josh Tycko, Michaela Hinks, Sita S. Chandrasekaran, Nicholas T. Perry, Julia Schaepe, Peter P. Du, Peter Lotfy, Michael C. Bassik, Lacramioara Bintu, Ami S. Bhatt, Patrick D. Hsu
Publikováno v:
Nature biotechnology, vol 41, iss 4
Large serine recombinases (LSRs) are DNA integrases that facilitate the site-specific integration of mobile genetic elements into bacterial genomes. Only a few LSRs, such as Bxb1 and PhiC31, have been characterized to date, with limited efficiency as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7983a88ff2526a58776722850e8f4007
https://escholarship.org/uc/item/6sp8538c
https://escholarship.org/uc/item/6sp8538c
Autor:
Jason Ohlstein, Julio Sanmiguel, Marco Crosariol, Anna Tretiakova, Stefan Worgall, Maria P. Limberis, Virginie S. Adam, Josh Tycko, Soumitra Roy, James M. Wilson
Publikováno v:
Human Gene Therapy. 32:1450-1456
Infants and older adults are especially vulnerable to infection by respiratory syncytial virus (RSV), which can cause significant illness and irreparable damage to the lower respiratory tract and for which an effective vaccine is not readily availabl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ffa97567c10c6d747ce586e662c14b5
https://doi.org/10.1089/hum.2021.079
https://doi.org/10.1089/hum.2021.079
Autor:
Adi X. Mukund, Josh Tycko, Sage J. Allen, Stephanie A. Robinson, Cecelia Andrews, Connor H. Ludwig, Kaitlyn Spees, Michael C. Bassik, Lacramioara Bintu
Despite growing knowledge of the functions of individual human transcriptional effector domains, much less is understood about how multiple effector domains within the same protein combine to regulate gene expression. Here, we measure transcriptional
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6caeb936c9eac3bb2ed497efb5fe771c