A conserved mechanism for regulating replisome disassembly in eukaryotes

Autor: Tom D Deegan, Karim Labib, Giuseppe Cannone, Michael Jenkyn-Bedford, Joseph T.P. Yeeles, Yasemin Baris, Morgan L. Jones

Publikováno v: Nature
Jenkyn-Bedford, M, Jones, M L, Baris, Y, Labib, K P M, Cannone, G, Yeeles, J T P & Deegan, T D 2021, ' A Conserved Mechanism for Regulating Replisome Disassembly in Eukaryotes ', Nature . https://doi.org/10.1038/s41586-021-04145-3

Replisome disassembly is the final step of eukaryotic DNA replication and is triggered by ubiquitylation of the CDC45–MCM–GINS (CMG) replicative helicase1–3. Despite being driven by evolutionarily diverse E3 ubiquitin ligases in different eukar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3762dde9c47e717fe2f32dd809798c47
https://doi.org/10.1038/s41586-021-04145-3

An updated perspective on the polymerase division of labor during eukaryotic DNA replication

Autor: Thomas A. Guilliam, Joseph T.P. Yeeles

Publikováno v: Critical Reviews in Biochemistry and Molecular Biology. 55:469-481

In eukaryotes three DNA polymerases (Pols), α, δ, and ε, are tasked with bulk DNA synthesis of nascent strands during genome duplication. Most evidence supports a model where Pol α initiates DNA synthesis before Pol ε and Pol δ replicate the le

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77d477008d72845ffeabc928a9e810f2
https://doi.org/10.1080/10409238.2020.1811630

Checkpoint-mediated DNA polymerase ε exonuclease activity curbing counteracts resection-driven fork collapse

Autor: Dolores Jurado-Santiago, Xingyu Yin, Marcus B. Smolka, Katsuhiko Shirahige, Sara Villa-Hernández, Joseph T.P. Yeeles, Miguel Garcia-Diaz, Michael Charles Lanz, Michele Giannattasio, Grazia Pellicanò, Mohammed Al Mamun, Rodrigo Bermejo

Publikováno v: Mol Cell
Digital.CSIC. Repositorio Institucional del CSIC
instname
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)

62 p.-7 fig.-1 tab.-7 fig. suppl.-1 tab.supl.
DNA polymerase ε (Polε) carries out high-fidelity leading strand synthesis owing to its exonuclease activity. Polε polymerase and exonuclease activities are balanced, because of partitioning of na

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8d7c5826d85b9afe139ae1e13cdaabb
http://hdl.handle.net/10261/239827

How the Eukaryotic Replisome Achieves Rapid and Efficient DNA Replication

Autor: Joseph T.P. Yeeles, Anne Early, Agnieszka Janska, John F.X. Diffley

Publikováno v: Molecular Cell

Summary The eukaryotic replisome is a molecular machine that coordinates the Cdc45-MCM-GINS (CMG) replicative DNA helicase with DNA polymerases α, δ, and ε and other proteins to copy the leading- and lagging-strand templates at rates between 1 and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::317c90c73bd3c6de1d0b28d6e5886ba5
https://doi.org/10.1016/j.molcel.2016.11.017

Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates

Autor: John F.X. Diffley, Christoph F. Kurat, Anne Early, Joseph T.P. Yeeles, Harshil Patel

Publikováno v: Molecular Cell

Summary The integrity of eukaryotic genomes requires rapid and regulated chromatin replication. How this is accomplished is still poorly understood. Using purified yeast replication proteins and fully chromatinized templates, we have reconstituted th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::310f2353d734965409ab19de0c9a8ede