Zobrazeno 1 - 10
of 70
pro vyhledávání: '"Joseph M. Jacobson"'
Autor:
Lin Zhao, Sabrina R. T. Koseki, Rachel A. Silverstein, Nadia Amrani, Christina Peng, Christian Kramme, Natasha Savic, Martin Pacesa, Tomás C. Rodríguez, Teodora Stan, Emma Tysinger, Lauren Hong, Vivian Yudistyra, Manvitha R. Ponnapati, Joseph M. Jacobson, George M. Church, Noah Jakimo, Ray Truant, Martin Jinek, Benjamin P. Kleinstiver, Erik J. Sontheimer, Pranam Chatterjee
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-8 (2023)
Abstract CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-programmed target site, limiting their sequence accessibility for robust genome editing applications. In this study, we recombine the PAM-interacting doma
Externí odkaz:
https://doaj.org/article/c6eaa1afc2b34657a91e037a58ba1052
Autor:
Pranam Chatterjee, Manvitha Ponnapati, Christian Kramme, Alexandru M. Plesa, George M. Church, Joseph M. Jacobson
Publikováno v:
Communications Biology, Vol 3, Iss 1, Pp 1-8 (2020)
Pranam Chatterjee et al. present a novel computational platform for engineering peptide fusions that bind to the SARS-CoV-2 spike protein and tag it for proteasomal degradation. They experimentally validate an optimal variant in human cells, showing
Externí odkaz:
https://doaj.org/article/c38f96c2ae5f4bc7940b60f8c64fa945
Autor:
Pranam Chatterjee, Jooyoung Lee, Lisa Nip, Sabrina R. T. Koseki, Emma Tysinger, Erik J. Sontheimer, Joseph M. Jacobson, Noah Jakimo
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-6 (2020)
Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targe
Externí odkaz:
https://doaj.org/article/de6c445336ca4aacb34b61cb8daeb358
Autor:
Joseph M. Jacobson, James W Weis
Publikováno v:
Nature Biotechnology. 39:1300-1307
The scientific ecosystem relies on citation-based metrics that provide only imperfect, inconsistent and easily manipulated measures of research quality. Here we describe DELPHI (Dynamic Early-warning by Learning to Predict High Impact), a framework t
Autor:
Christian Kramme, Pranam Chatterjee, Alexandru M Plesa, George M. Church, Joseph M. Jacobson, Manvitha Ponnapati
Publikováno v:
Communications Biology, Vol 3, Iss 1, Pp 1-8 (2020)
Communications Biology
Communications Biology
The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, has elicited a global health crisis of catastrophic proportions. With only a few vaccines approved for early or limited use, there is a critical need for effective antiviral strategie
Autor:
Erik J. Sontheimer, Emma Tysinger, Noah Jakimo, Jooyoung Lee, Pranam Chatterjee, Lisa Nip, Sabrina R. T. Koseki, Joseph M. Jacobson
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-6 (2020)
Nature Communications
Nature Communications
CRISPR-associated (Cas) DNA-endonucleases are remarkably effective tools for genome engineering, but have limited target ranges due to their protospacer adjacent motif (PAM) requirements. We demonstrate a critical expansion of the targetable sequence
The novel coronavirus SARS-CoV-2 continues to pose a significant global health threat. Along with vaccines and targeted therapeutics, there is a critical need for rapid diagnostic solutions. In this work, we employ deep learning-based protein design
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::113333719c1a70503201936108308cce
https://doi.org/10.1101/2021.06.04.447114
https://doi.org/10.1101/2021.06.04.447114
Determining the structure of proteins has been a long-standing goal in biology. Language models have been recently deployed to capture the evolutionary semantics of protein sequences. Enriched with multiple sequence alignments (MSA), these models can
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::31bf3c4856c2cf55af570c26def6b6dc
https://doi.org/10.1101/2021.06.02.446809
https://doi.org/10.1101/2021.06.02.446809
The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, has elicited a global health crisis of catastrophic proportions. With no approved cure or vaccine currently available, there is a critical need for effective antiviral strategies. In
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::27066d72c40125c3e8959dbf910a0716
https://doi.org/10.1101/2020.06.01.127829
https://doi.org/10.1101/2020.06.01.127829
Autor:
James W, Weis, Joseph M, Jacobson
Publikováno v:
Nature biotechnology. 39(10)
The scientific ecosystem relies on citation-based metrics that provide only imperfect, inconsistent and easily manipulated measures of research quality. Here we describe DELPHI (Dynamic Early-warning by Learning to Predict High Impact), a framework t