Heparinase I (Neutralase) Reversal of Systemic Anticoagulation

Autor: King C. Lee, Joseph J. Zimmermann, Luis G. Michelsen, Mi Kyoung Lee, Jerrold H. Levy, Mutsuhito Kikura, Fania Szlam

Publikováno v: Anesthesiology. 85:339-346

Background Protamine causes multiple adverse reactions. Heparinase I, a specific enzyme that inactivates heparin, is a possible alternative to protamine. In this study, the authors examined the efficacy of heparinase I to reverse heparin-induced anti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83c4e1b5af13dea5605fb8b1ee99a2e4
https://doi.org/10.1097/00000542-199608000-00016

The release of heparinase from the periplasmic space of flavobacterium heparinum by three-step osmotic shock

Autor: Charles L. Cooney, Karen Oddie, Robert Langer, Joseph J. Zimmermann

Publikováno v: Applied Biochemistry and Biotechnology. 30:137-148

Heparinase was released from the periplasmic space of Flavobacterium heparinum by three-step osmotic shock procedure. The procedure involves resuspending exponentially growing cells consecutively into (1) 40% sucrose, (2) 10 mM sodium phosphate, 2 mM

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15651baaec94ad859757b9f41ba155e1
https://doi.org/10.1007/bf02921681

Intranasal immunization with a proteosome-adjuvanted SARS-CoV-2 spike protein-based vaccine is immunogenic and efficacious in mice and hamsters.

Autor: Stark FC; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Akache B; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada. Bassel.akache@nrc-cnrc.gc.ca., Deschatelets L; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Tran A; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Stuible M; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Durocher Y; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., McCluskie MJ; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Agbayani G; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Dudani R; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Harrison BA; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Renner TM; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Makinen SR; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Bavananthasivam J; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Duque D; National Research Council Canada, Human Health Therapeutics, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada., Gagne M; Biodextris Inc., 525 boul. Cartier West, Laval, QC, H7V 3S8, Canada., Zimmermann J; Inspirevax Inc., 46 rue de Saint-Tropez, Kirkland, QC, H9J 2K6, Canada., Zarley CD; Oragenics, Inc., 13700 Progress Blvd, Alachua, FL, 32608, USA.; CDZarley LLC., 133 Butternut Dr, Pottstown, PA, 19464, USA., Cochrane TR; Oragenics, Inc., 13700 Progress Blvd, Alachua, FL, 32608, USA.; BrevisRefero Corporation, 295 Alliance Road Suite 1B/C, Milton, ON, L9T 2X7, Canada., Handfield M; Oragenics, Inc., 13700 Progress Blvd, Alachua, FL, 32608, USA.

Publikováno v: Scientific reports [Sci Rep] 2022 Jun 13; Vol. 12 (1), pp. 9772. Date of Electronic Publication: 2022 Jun 13.