Zobrazeno 1 - 10
of 51
pro vyhledávání: '"Joseph Inselburg"'
Publikováno v:
Infection and Immunity. 61:2041-2047
We report the results of vaccination trial 2 of Panamanian Aotus monkeys with a recombinant blood-stage antigen, SERA 1, of the malaria parasite Plasmodium falciparum. Monkeys were immunized with SERA 1, a 262-amino-acid fragment (amino acids 24 to 2
Autor:
Philip J. Barr, Benjamin K. Hahm, Isabella A. Quakyi, Wasim A. Siddiqui, Joe Kansopon, Ian C. Bathurst, Pascal Millet, Sandra P. Chang, Helen L. Gibson, Joseph Inselburg, David C. Kaslow, Kim M. Green, George Hui
Publikováno v:
Vaccine. 11:449-456
Surface proteins from several different life-cycle stages of the malaria parasite Plasmodium falciparum were expressed at high levels in the yeast Saccharomyces cerevisiae. Purified proteins, both individually and in cocktails, were used to immunize
Publikováno v:
Journal of Biochemical and Biophysical Methods. 24:45-50
We have made a significant improvement in the electroelution device, Elutrap (Schleicher and Schuell) by substituting an agarose gel barrier, which is made from 0.6% agarose (SeaKem GTG; FMC Corporation), into the elution chamber in place of the manu
Autor:
I C Bathurst, J Kansopon, Joseph Inselburg, K. M. Green, P J Barr, R. N. Rossan, Wu-Bo Li, David J. Bzik, B. K. Hahm
Publikováno v:
Infection and Immunity. 59:1247-1250
We describe the vaccination of Panamanian monkeys (Aotus sp.) with two recombinant blood stage antigens that each contain a portion of the N-terminal region of the SERA (serine repeat antigen) protein of the malaria parasite Plasmodium falciparum. We
Publikováno v:
Molecular and Biochemical Parasitology. 39:127-134
The nucleotide sequence of the dihydrofolate reductase-thymidylate synthase (DHFR-TS) gene in pyrimethamine-resistant (PyrR) mutants of Plasmodium falciparum selected in vitro was examined to determine if specific mutations in DHFR were associated wi
Autor:
Junichi Watanabe, Joseph Inselburg
Publikováno v:
Molecular and biochemical parasitology. 65(2)
We isolated a panel of 20 DNA probes that hybridize specifically to chromosome No. 4 of Plasmodium falciparum and used them to construct a restriction map of chromosome No. 4 in the FCR3 strain. These probes were partially sequenced and had an insert
Publikováno v:
Molecular and biochemical parasitology. 64(1)
We report the isolation and sequence of genomic clones encoding a chaperonin 60 gene from the human malaria parasite Plasmodium falciparum . The gene contains a single intron of 868 nucleotides, the largest yet identified in this organism. The readin
Autor:
Joseph Inselburg, Susan Lee
Publikováno v:
The Journal of parasitology. 79(5)
The in vitro sensitivity of Plasmodium falciparum to bleomycin, busulfan, camptothecin, CC-1065, cisplatin, daunomycin, distamycin, luzopeptin, mAMSA, mitomycin C, naladixic acid, U71184, VM26, and VP16, or combinations of them was examined. The leth
Publikováno v:
Molecular and biochemical parasitology. 61(1)
Plasmodium species possess developmentally regulated ribosomal RNA (rRNA) genes. This report describes the expression and gene structure of the largest subunit of P. falciparum RNA polymerase I (RNAPI), which is responsible for the synthesis of rRNA.
Publikováno v:
Infection and immunity. 61(5)
We describe the third of three vaccination trials of Panamanian Aotus monkeys with a recombinant blood-stage antigen derived from the malaria parasite Plasmodium falciparum. Immunization was performed with an N-terminal region of the SERA antigen (se