Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Joseph E. Doherty"'
Publikováno v:
The FASEB Journal. 27:3753-3762
Transposons permit permanent cellular genome engineering in vivo. However, transgene expression falls rapidly postdelivery due to a variety of mechanisms, including immune responses. We hypothesized that delaying initial transgene expression would im
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af182922efb12b6287ba0ad31327b00a
https://doi.org/10.1096/fj.13-232090
https://doi.org/10.1096/fj.13-232090
Autor:
Leslie E. Huye, Nancy L. Craig, Matthew H. Wilson, Joseph E. Doherty, Kosuke Yusa, Liqin Zhou
Publikováno v:
Human Gene Therapy. 23:311-320
We characterized a recently developed hyperactive piggyBac (pB) transposase enzyme [containing seven mutations (7pB)] for gene transfer in human cells in vitro and to somatic cells in mice in vivo. Despite a protein level expression similar to that o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d546dab97bc36b079dbf22eecd907a1e
https://doi.org/10.1089/hum.2011.138
https://doi.org/10.1089/hum.2011.138
Autor:
Matthew H. Wilson, Sai K. Saridey, Li Liu, Daniel L. Galvan, Aparna Kaja, Bradley S. Fletcher, Joseph E. Doherty
Publikováno v:
Molecular Therapy. 17(12):2115-2120
Somatic cell gene transfer has permitted inducible gene expression in vivo through coinfection of multiple viruses. We hypothesized that the highly efficient plasmid-based piggyBac transposon system would enable long-term inducible gene expression in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::549536220f773c447cd023a45c1ae2b8
Autor:
Matthew H. Wilson, Cliona M. Rooney, Daniel L. Galvan, Joseph E. Doherty, Leslie E. Huye, Yozo Nakazawa, Sunandan Saha
Publikováno v:
Journal of Visualized Experiments.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b59bc2ef233909f9de6a014d976e39ab
https://doi.org/10.3791/4235-v
https://doi.org/10.3791/4235-v
Autor:
Cliona M. Rooney, Matthew H. Wilson, Leslie E. Huye, Sunandan Saha, Daniel L. Galvan, Yozo Nakazawa, Joseph E. Doherty
Publikováno v:
Journal of Visualized Experiments.
The piggyBac transposon system is naturally active, originally derived from the cabbage looper moth. This non-viral system is plasmid based, most commonly utilizing two plasmids with one expressing the piggyBac transposase enzyme and a transposon pla
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0b560ea396af3bca9f74bc9935c0c176
https://doi.org/10.3791/4235
https://doi.org/10.3791/4235