Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Joseph Abraham Christopher John"'
Autor:
Joseph Abraham Christopher John, Chiao-Hwa Yeh, Yi-Jen Huang, Ya-Nan Chang, Wen-Ya Chen, Chi-Yao Chang, Pei-Wen Lin, Wen-Huei Tsui, Fu-Pang Lin, San-Tai Shen, Chung-Hsiang Yuan
Publikováno v:
Apoptosis. 13:165-176
The grouper iridovirus (GIV) belongs to the family Iridoviridae, whose genome contains an antiapoptotic B-cell lymphoma (Bcl)-2-like gene. This study was carried-out to understand whether GIV blocks apoptosis in its host. UV-irradiated grouper kidney
Autor:
Cheng-Hui Lin, Joseph Abraham Christopher John, Chi-Yao Chang, Chih-Hung Lin, Chiou-Yueh Lee, Chao-An Cheng, Ming-Shan Wu
Publikováno v:
Veterinary Immunology and Immunopathology. 109:255-265
Immunoglobulin M (IgM) from the whole serum of grouper fish, Epinephelus coioides was purified by affinity chromatography using protein A-Sepharose column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions
Autor:
Jun-Chang Chen, Chi-Yao Chang, Li Wei Ou, Chih-Hung Lin, Cheng-Hui Lin, Joseph Abraham Christopher John
Publikováno v:
Aquatic Toxicology. 66:111-124
Metallothionein (MT) has been used widely as a potential molecular marker to detect the deleterious effects of heavy metals in aquatic ecosystem. Here we exposed ayu, Plecoglossus altivelis, to zinc (Zn) and tested the distribution as well as the ind
Autor:
Yu Shen Lai, Shih Chieh Chen, Kang Fang, Joseph Abraham Christopher John, Ing Cherng Guo, Chi Yao Chang
Publikováno v:
Vaccine. 20:3221-3229
Mouse monoclonal antibody (MAb-18) against yellow grouper nervous necrosis virus (YGNNV) coat protein was developed and assayed for its neutralization ability. In the present study, we cloned and sequenced the cDNAs encoding heavy (gamma) and light (
Publikováno v:
Biochemical and Biophysical Research Communications. 291:798-805
Metal-responsive transcription factor, MTF-1 is a zinc finger protein, shown to be essential for embryonic development. Homozygous knockout mouse embryos for MTF-1 die in utero at day 14 of gestation, due to liver decay. In the present study, we repo
Publikováno v:
Environmental toxicology and chemistry. 26(1)
Metallothionein is a small (6-kDa), cysteine-rich protein expressed by a six-zinc finger protein called metal-responsive element-binding transcription factor-1 (MTF-1) in response to Zn and Cd. Our previous reports have shown the basal expression of
Publikováno v:
Biochemical and biophysical research communications. 351(2)
Mx proteins are interferon induced, antiviral proteins, expressed in response to treatment with double stranded RNA or virus infection. Here we report the cloning, sequencing, and antiviral property of three forms of Mx genes, MxI, MxII, and MxIII fr
Autor:
Cheng-Hui Lin, Hui-Fen Lin, Chih-Hung Lin, Shao-Chun Wu, Wen-Ya Chen, Chi-Yao Chang, Joseph Abraham Christopher John
Publikováno v:
Aquatic toxicology (Amsterdam, Netherlands). 69(3)
Metallothionein (Mt) has been considered as a molecular marker of metal pollution in aquatic ecosystems. Less is known about the expression of mt gene during embryogenesis. Here, we report the cloning, sequencing, and the expression pattern of mt gen
Autor:
Cheng-Hui Lin, Chi Yao Chang, I. C. Guo, Kang Fang, S. C. Chen, Y. S. Lai, Joseph Abraham Christopher John
Publikováno v:
Journal of fish diseases. 26(1)
Four tropical marine fish cell lines have been established from the eye, fin, heart and swim bladder of grouper, Epinephelus awoara (Temminck & Schlegel). Optimum media and temperature conditions for maximum growth were standardized. The eye and swim
Publikováno v:
Protein expression and purification. 24(2)
The pituitary-specific transcription factor Pit-1 belongs to the family of POU-domain proteins and is known to play an important role in the differentiation of pituitary cells. Here we report the complete nucleotide sequence of cDNA encoding Pit-1 fr