Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Joseph A. Affholter"'
Publikováno v:
South East European Journal of Economics and Business, Vol 16, Iss 2, Pp 82-100 (2021)
The paper contributes to the understanding of entrepreneurial activity by assessing the relative importance of eight entrepreneurial readiness factors across countries in different regions and stages of economic development. Drawing on the literature
The World Bank, the World Economic Forum, the Heritage Foundation, and other global organizations have studied national business climates to determine optimal conditions and practices necessary to stimulate entrepreneurship and economic growth. This
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b42c5f81739083df971dd42e02c3853
https://doi.org/10.4018/978-1-4666-7533-9.ch001
https://doi.org/10.4018/978-1-4666-7533-9.ch001
Autor:
Joseph A. Affholter, Robert L. Karvonen, Paul H. Wooley, Inocencio A. Cuesta, Felix Fernandez-Madrid, Sudha Sud, Zheng Song
Publikováno v:
Annals of the New York Academy of Sciences. 756:195-198
Autor:
Joseph A. Affholter, Anne E. De Vaux, Victor J. Hruby, Tomi K. Sawyer, Mac E. Hadley, D J Staples, Ana Maria de Lauro Castrucci
Publikováno v:
Annals of the New York Academy of Sciences. 680:597-599
Autor:
Lynn Kan, Ian Holmes, John F. Schindler, Ruth A. Richard, Clifford J. Unkefer, Janet Newman, Paul E. Swanson, Thomas C. Terwilliger, Joseph A. Affholter, Thomas S. Peat
Publikováno v:
Biochemistry. 38(49)
The hydrolytic haloalkane dehalogenases are promising bioremediation and biocatalytic agents. Two general classes of dehalogenases have been reported from Xanthobacter and Rhodococcus. While these enzymes share 30% amino acid sequence identity, they
Publikováno v:
Nature biotechnology. 16(3)
We have developed a simple and efficient method for in vitro mutagenesis and recombination of polynucleotide sequences. The staggered extension process (StEP) consists of priming the template sequence(s) followed by repeated cycles of denaturation an
Publikováno v:
Nature Biotechnology. 17:333-334
Identification of residues in the insulin molecule important for binding to insulin-degrading enzyme
Autor:
Richard A. Roth, Margaret A. Cascieri, Marvin L. Bayne, Monika Casaretto, Joseph A. Affholter, Jens Jorgen Veilgaard Brange
Publikováno v:
Biochemistry. 29(33)
Insulin-degrading enzyme (IDE) hydrolyzes insulin at a limited number of sites. Although the positions of these cleavages are known, the residues of insulin important in its binding to IDE have not been defined. To this end, the authors have studied
Publikováno v:
Molecular endocrinology (Baltimore, Md.). 4(8)
We have recently described the isolation of a cDNA encoding an enzyme thought to be involved in the degradation of insulin by insulin-responsive tissues. This enzyme, referred to as insulin-degrading enzyme (IDE), is a cytosolic proteinase of 110,000
Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III
Publikováno v:
Science (New York, N.Y.). 242(4884)
A proteinase with high affinity for insulin has been proposed to play a role in the cellular processing of this hormone. A complementary DNA (cDNA) coding for this enzyme has been isolated and sequenced. The deduced amino acid sequence of the enzyme