Lateral membrane organization as target of an antimicrobial peptidomimetic compound

Autor: Adéla Melcrová, Sourav Maity, Josef Melcr, Niels A. W. de Kok, Mariella Gabler, Jonne van der Eyden, Wenche Stensen, John S. M. Svendsen, Arnold J. M. Driessen, Siewert J. Marrink, Wouter H. Roos

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-13 (2023)

Abstract Antimicrobial resistance is one of the leading concerns in medical care. Here we study the mechanism of action of an antimicrobial cationic tripeptide, AMC-109, by combining high speed-atomic force microscopy, molecular dynamics, fluorescenc

Externí odkaz: https://doaj.org/article/dc03a19cfff44e7683a6bac15ecc3668

Quantitative linear dichroism imaging of molecular processes in living cells made simple by open software tools

Autor: Alexey Bondar, Olga Rybakova, Josef Melcr, Jan Dohnálek, Petro Khoroshyy, Ondřej Ticháček, Štěpán Timr, Paul Miclea, Alina Sakhi, Vendula Marková, Josef Lazar

Publikováno v: Communications Biology, Vol 4, Iss 1, Pp 1-12 (2021)

Expanding on their previous work, Bondar et al present open source software tools to reliably quantify linear dichroism and determine molecular orientations. They demonstrate the utility of the tools by imaging synthetic lipid vesicles and as well as

Externí odkaz: https://doaj.org/article/1ddcc28075a347aea94ccfb507f7c9df

Martini 3

Autor: Vincent Nieto, Valentina Corradi, Siewert J. Marrink, Matti Javanainen, Peter C. Kroon, Ilpo Vattulainen, Hector Martinez-Seara, D. Peter Tieleman, Alex H. de Vries, Jan Domański, Hanif M. Khan, Robert B. Best, Ilias Patmanidis, Sebastian Thallmair, Ignacio Faustino, Xavier Periole, Jonathan Barnoud, Tsjerk A. Wassenaar, Josef Melcr, Nathalie Reuter, Haleh Abdizadeh, Bart M H Bruininks, Paulo C. T. Souza, Fabian Grünewald, Riccardo Alessandri, Luca Monticelli

Publikováno v: Nature Methods, 18, 382-388. Nature Publishing Group
Nature Methods
Souza, P C T, Alessandri, R, Barnoud, J, Thallmair, S, Faustino, I, Grünewald, F, Patmanidis, I, Abdizadeh, H, Bruininks, B M H, Wassenaar, T A, Kroon, P C, Melcr, J, Nieto, V, Corradi, V, Khan, H M, Domański, J, Javanainen, M, Martinez-Seara, H, Reuter, N, Best, R B, Vattulainen, I, Monticelli, L, Periole, X, Tieleman, D P, de Vries, A H & Marrink, S J 2021, ' Martini 3 : a general purpose force field for coarse-grained molecular dynamics ', Nature Methods, vol. 18, no. 4, pp. 382-388 . https://doi.org/10.1038/s41592-021-01098-3

The coarse-grained Martini force field is widely used in biomolecular simulations. Here we present the refined model, Martini 3 (http://cgmartini.nl), with an improved interaction balance, new bead types and expanded ability to include specific inter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1fe1d2646ea2c4aa330b9fd3b0ab46d
https://hdl.handle.net/11370/27f1d65b-ff4d-4290-9fc8-b019e36c5c2e

Membrane thickness, lipid phase and sterol type are determining factors in the permeability of membranes to small solutes

Autor: Bert Poolman, Jacopo Frallicciardi, Pareskevi Siginou, Siewert J. Marrink, Josef Melcr

Publikováno v: Nature Communications, 13(1):1605. Nature Publishing Group
bioRxiv

Cell membranes provide a selective semi-permeable barrier to the passive transport of molecules. This property differs greatly between organisms. While the cytoplasmic membrane of bacterial cells is highly permeable for weak acids and glycerol, yeast

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01228bef1d6395bab08fb92bfdc7b7a5
https://doi.org/10.1038/s41467-022-29272-x

Adaptive response to wine selective pressures shapes the genome of a Saccharomyces interspecies hybrid

Autor: Sarah J Routledge, John A. Linney, Eladio Barrio, Josef Melcr, Andrew R. Pitt, Gabriel L. Castiglioni, Javier Alonso-del-Real, Amparo Querol, María Lairón-Peris, Alan D. Goddard

Publikováno v: Lairón-Peris, M, Castiglioni, G L, Routledge, S J, Alonso-del-Real, J, Linney, J A, Pitt, A R, Melcr, J, Goddard, A D, Barrio, E & Querol, A 2021, ' Adaptive response to wine selective pressures shapes the genome of a Saccharomyces interspecies hybrid ', Microbial genomics . https://doi.org/10.1099/mgen.0.000628
Microbial genomics, 7(8):000628. AMER SOC MICROBIOLOGY
Microbial Genomics
Digital.CSIC. Repositorio Institucional del CSIC
instname

During industrial processes, yeasts are exposed to harsh conditions, which eventually lead to adaptation of the strains. In the laboratory, it is possible to use experimental evolution to link the evolutionary biology response to these adaptation pre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad39fdc02942a45c8328162e4681d9cf
https://publications.aston.ac.uk/id/eprint/42982/1/Adaptive_response_to_wine_selective_pressures.pdf