Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography

Autor: Suraj Pandey, George Calvey, Andrea M. Katz, Tek Narsingh Malla, Faisal H. M. Koua, Jose M. Martin-Garcia, Ishwor Poudyal, Jay-How Yang, Mohammad Vakili, Oleksandr Yefanov, Kara A. Zielinski, Sasa Bajt, Salah Awel, Katarina Doerner, Matthias Frank, Luca Gelisio, Rebecca Jernigan, Henry Kirkwood, Marco Kloos, Jayanath Koliyadu, Valerio Mariani, Mitchell D. Miller, Grant Mills, Garrett Nelson, Jose L. Olmos Jr, Alireza Sadri, Tokushi Sato, Alexandra Tolstikova, Weijun Xu, Abbas Ourmazd, John C. H. Spence, Peter Schwander, Anton Barty, Henry N. Chapman, Petra Fromme, Adrian P. Mancuso, George N. Phillips Jr, Richard Bean, Lois Pollack, Marius Schmidt

Publikováno v: IUCrJ, Vol 8, Iss 6, Pp 878-895 (2021)

Here, we illustrate what happens inside the catalytic cleft of an enzyme when substrate or ligand binds on single-millisecond timescales. The initial phase of the enzymatic cycle is observed with near-atomic resolution using the most advanced X-ray s

Externí odkaz: https://doaj.org/article/fbdbd2a5c401447a974071c858ead28e

Structural enzymology using X-ray free electron lasers

Autor: Christopher Kupitz, Jose L. Olmos Jr., Mark Holl, Lee Tremblay, Kanupriya Pande, Suraj Pandey, Dominik Oberthür, Mark Hunter, Mengning Liang, Andrew Aquila, Jason Tenboer, George Calvey, Andrea Katz, Yujie Chen, Max O. Wiedorn, Juraj Knoska, Alke Meents, Valerio Majriani, Tyler Norwood, Ishwor Poudyal, Thomas Grant, Mitchell D. Miller, Weijun Xu, Aleksandra Tolstikova, Andrew Morgan, Markus Metz, Jose M. Martin-Garcia, James D. Zook, Shatabdi Roy-Chowdhury, Jesse Coe, Nirupa Nagaratnam, Domingo Meza, Raimund Fromme, Shibom Basu, Matthias Frank, Thomas White, Anton Barty, Sasa Bajt, Oleksandr Yefanov, Henry N. Chapman, Nadia Zatsepin, Garrett Nelson, Uwe Weierstall, John Spence, Peter Schwander, Lois Pollack, Petra Fromme, Abbas Ourmazd, George N. Phillips Jr., Marius Schmidt

Publikováno v: Structural Dynamics, Vol 4, Iss 4, Pp 044003-044003-7 (2017)

Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with a substrate just prior to being probed by an X-ray pulse. This approach offers several advantages

Externí odkaz: https://doaj.org/article/c3a9db9c4e10400295db263c10c7a4c9

Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography

Autor: Henry N. Chapman, Katarina Doerner, Andrea M. Katz, Valerio Mariani, Adrian P. Mancuso, Weijun Xu, Anton Barty, Kara A. Zielinski, Jose L. Olmos, Luca Gelisio, Petra Fromme, Grant Mills, Tokushi Sato, Rebecca Jernigan, Oleksandr Yefanov, George D. Calvey, Mohammad Vakili, Mitchell D. Miller, Alireza Sadri, Henry Kirkwood, Ishwor Poudyal, Richard Bean, Tek Narsingh Malla, Jay-How Yang, John C. H. Spence, Peter Schwander, Jayanath Koliyadu, Lois Pollack, A. Tolstikova, Abbas Ourmazd, Suraj Pandey, Saša Bajt, Garrett Nelson, Faisal Hammad Mekky Koua, Matthias Frank, George N. Phillips, Salah Awel, Marius Schmidt, Jose M. Martin-Garcia, Marco Kloos

Publikováno v: IUCrJ 8(6), 878-895 (2021). doi:10.1107/S2052252521008125
IUCrJ, Vol 8, Iss 6, Pp 878-895 (2021)
IUCrJ, vol 8, iss Pt 6
Digital.CSIC. Repositorio Institucional del CSIC
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IUCrJ

18 pags, 11 figs, 5 tabs
Here, we illustrate what happens inside the catalytic cleft of an enzyme when substrate or ligand binds on single-millisecond timescales. The initial phase of the enzymatic cycle is observed with near-atomic resolution u

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92618450b5a44ce38d586bcfe7458e06
https://bib-pubdb1.desy.de/record/473701

Prochlorococcusphage ferredoxin: structural characterization and electron transfer to cyanobacterial sulfite reductases

Autor: Dimithree Kahanda, George N. Phillips, George N. Bennett, Jose L. Olmos, Othneil N. Sparks, Joshua T. Atkinson, Jonathan J. Silberg, Weijun Xu, Mitchell D. Miller, Ian J. Campbell

Marine cyanobacteria are infected by phage whose genomes encode ferredoxin (Fd) electron carriers. While these Fds are thought to redirect the energy harvested from light to phage-encoded oxidoreductases that enhance viral fitness, it is not clear ho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::15f2a7676dca845f04ee223be0dd94a3
https://doi.org/10.1101/2020.02.07.937771

Enzyme intermediates captured 'on the fly' by mix-and-inject serial crystallography

Autor: Saša Bajt, Shatabdi Roy-Chowdhury, David Xu, Max O. Wiedorn, Nirupa Nagaratnam, Marius Schmidt, Matthew Seaberg, R. Fung, Christopher Kupitz, Jose M. Martin-Garcia, Jesse Coe, Ishwor Poudyal, James Zook, Lee Tremblay, Anton Barty, Raimund Fromme, Dominik Oberthuer, Mark S. Hunter, Peter Schwander, Mengning Liang, Jose L. Olmos, Euiyoung Bae, Thomas D. Grant, Mark R. Holl, Michael Heyman, Juraj Knoska, Stephan Stern, Ganesh Subramanian, Mitchell D. Miller, Kanupriya Pande, Matthias Frank, Lois Pollack, John C. H. Spence, Abbas Ourmazd, Yun Zhao, Jason E. Koglin, Oleksandr Yefanov, George D. Calvey, Valerio Mariani, Petra Fromme, Jacob Verburgt, Garrett Nelson, George N. Phillips, Suraj Pandey, Uwe Weierstall, Andrea M. Katz, Nadia A. Zatsepin, Thomas A. White, Henry N. Chapman, Tyler Norwood

Publikováno v: Olmos, JL; Pandey, S; Martin-Garcia, JM; Calvey, G; Katz, A; Knoska, J; et al.(2018). Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography. BMC Biology, 16(1). doi: 10.1186/s12915-018-0524-5. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/5vf7b95d
BMC Biology, Vol 16, Iss 1, Pp 1-15 (2018)
BMC biology, vol 16, iss 1
BMC biology 16(1), 59 (2018). doi:10.1186/s12915-018-0524-5
BMC Biology

Background Ever since the first atomic structure of an enzyme was solved, the discovery of the mechanism and dynamics of reactions catalyzed by biomolecules has been the key goal for the understanding of the molecular processes that drive life on ear

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b923c143ddeddd22834374dd18b1d36
http://www.escholarship.org/uc/item/5vf7b95d