Effect of α-Glucosylation on the Stability, Antioxidant Properties, Toxicity, and Neuroprotective Activity of (–)-Epigallocatechin Gallate

Autor: Jose L. Gonzalez-Alfonso, Pablo Peñalver, Antonio O. Ballesteros, Juan C. Morales, Francisco J. Plou

Publikováno v: Frontiers in Nutrition, Vol 6 (2019)

(–)-Epigallocatechin gallate (EGCG), the predominant catechin (≥50%) in green tea (Camellia sinensis), displays several bioactive properties but its stability and bioavailability are low. In this work, the properties of two α-glucosyl derivative

Externí odkaz: https://doaj.org/article/5a2891f7a1ae43caafecd46027d28256

Thermophilic carboxylesterases from hydrothermal vents of the volcanic island of Ischia active on synthetic and biobased polymers and mycotoxins

Autor: Marco A. Distaso, Tatyana N. Chernikova, Rafael Bargiela, Cristina Coscolín, Peter Stogios, Jose L. Gonzalez-Alfonso, Sofia Lemak, Anna N. Khusnutdinova, Francisco J. Plou, Elena Evdokimova, Alexei Savchenko, Evgenii A. Lunev, Michail M. Yakimov, Olga V. Golyshina, Manuel Ferrer, Alexander F. Yakunin, Peter N. Golyshin

Publikováno v: bioRxiv

Hydrothermal vents have a widespread geographical distribution and are of high interest for investigating microbial communities and robust enzymes for various industrial applications. We examined microbial communities and carboxylesterases of two ter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4620bb4494157cebf8292f8f896dec0f
https://doi.org/10.1101/2022.09.17.508236

Enzymatic Synthesis of Phloretin α-Glucosides Using a Sucrose Phosphorylase Mutant and its Effect on Solubility, Antioxidant Properties and Skin Absorption

Autor: Jesús Jiménez-Barbero, Jose L. Gonzalez-Alfonso, Tom Desmet, Julia Sanz-Aparicio, Zorica Ubiparip, Cristina Alonso, Elena Jiménez-Ortega, Antonio Ballesteros, Ana Poveda, Francisco J. Plou, Luisa Coderch

Publikováno v: ADVANCED SYNTHESIS & CATALYSIS
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
Digital.CSIC. Repositorio Institucional del CSIC
instname

Glycosylation of polyphenols may increase their aqueous solubility, stability, bioavailability and pharmacological activity. Herein, we used a mutant of sucrose phosphorylase from Thermoanaerobacterium thermosaccharolyticum engineered to accept large

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::decefe761cb9e234960e72e1105fbbc8
http://hdl.handle.net/10261/242256

Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis

Autor: Sandra Alonso, Gerard Santiago, Isabel Cea-Rama, Laura Fernandez-Lopez, Cristina Coscolín, Jan Modregger, Anna K. Ressmann, Mónica Martínez-Martínez, Helena Marrero, Rafael Bargiela, Marcos Pita, Jose L. Gonzalez-Alfonso, Manon L. Briand, David Rojo, Coral Barbas, Francisco J. Plou, Peter N. Golyshin, Patrick Shahgaldian, Julia Sanz-Aparicio, Víctor Guallar, Manuel Ferrer

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
instname

Enzyme engineering has allowed not only the de novo creation of active sites catalysing known biological reactions with rates close to diffusion limits, but also the generation of abiological sites performing new-to-nature reactions. However, the cat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b52949ec783ab8d11f2be711964a22e4
http://hdl.handle.net/10261/218885

Production and Surfactant Properties of Tert-Butyl α-d-Glucopyranosides Catalyzed by Cyclodextrin Glucanotransferase

Autor: Francisco J. Plou, Manuel Bernabé, Jose L. Gonzalez-Alfonso, Antonio Ballesteros, Humberto García-Arellano, José-Luis Parra, Miguel Alcalde, Francesc Comelles, Claudia Ubilla

Publikováno v: Catalysts
Volume 9
Issue 7
Catalysts, Vol 9, Iss 7, p 575 (2019)
Digital.CSIC. Repositorio Institucional del CSIC
instname

While testing the ability of cyclodextrin glucanotransferases (CGTases) to glucosylate a series of flavonoids in the presence of organic cosolvents, we found out that this enzyme was able to glycosylate a tertiary alcohol (tert-butyl alcohol). In par

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::569838a06fd12ade645a80a12dea1ceb