Zobrazeno 1 - 8
of 8
pro vyhledávání: '"JoséFranco da Silveira"'
Publikováno v:
Molecular and Biochemical Parasitology. 65:161-169
We have cloned and sequenced a cDNA clone coding for a metacyclic trypomastigote-specific surface glycoprotein with a molecular mass of 82 kDa (MTS-gp82). By immunoblotting and immunoprecipitation, antibodies against the recombinant protein recognize
Autor:
Nobuko Yoshida, Luiz Juliano, JoséFranco da Silveira, Fabio R. Santori, Miriam Leandro Dorta, Maria A. Juliano, Rita Ruiz
Publikováno v:
Molecular and biochemical parasitology. 78(1-2)
Recombinant proteins and synthetic peptides representing various sequences of gp82, a surface glycoprotein of Trypanosoma cruzi metacyclic trypomastigotes implicated in mammalian cell invasion, were used in this study aiming at the identification of
Autor:
Martín Vázquez, Arantxa Cortés, Mariano J. Levin, Bianca Zingales, Arthur Gruber, Antonio González, José Luis Ramírez, Maria Isabel Nogueira Cano, JoséFranco da Silveira, Carlos Alonso, JoséM. Requena, Edson Rondinelli, Wim Degrave
Publikováno v:
Molecular and biochemical parasitology. 71(2)
a Escola Paul&a de Medicina, Rua Botucatu, 862, CEP 04023-062, Stio Pa&o, Brazil b Institute de Quimica da USP, Sao Paula, Brazil ’ Institute de Inuestigaciones en Engenharia Genetica y Biologia Molecular, Buenos Aires. Argentina ’ Instituto de P
Autor:
Estela Maria Novak, JoséFranco da Silveira, Henrique B.M. Gomes, Palmira Guevara, Maricilda Palandi de Mello, Iván Galindo, JoséL. Ramirez
Publikováno v:
Molecular and biochemical parasitology. 60(2)
A fragment of Trypanosoma cruzi ribosomal intergenic spacer (IGS) located at 6.7 kb from the 3′ end of the 24S rRNA gene was analyzed. This IGS fragment is characterized by the presence of three types of repetitive elements (designated Spacer Repet
Publikováno v:
Experimental Parasitology. 64:354-360
Intact RNAs were isolated from epimastigote forms of different Trypanosoma cruzi strains. Translation of the mRNAs using rabbit reticulocyte lysate and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed protein profiles comp
Publikováno v:
Molecular and Biochemical Parasitology. 7:159-172
Intact RNAs were isolated from Plasmodium chabaudi growing synchronously in mice at different stages of the erythrocytic cycle. Translation of the mRNAs using rabbit reticulocyte lysate showed stage-specific patterns comparable to those observed in v
Publikováno v:
Molecular and biochemical parasitology. 11
Several Plasmodium chabaudi antigens are synthesized and the corresponding mRNAs are detected by cell-free translation only during specific stages of the erythrocytic cycle. Ring stage mRNA was used to construct a cDNA library in the plasmid vector p
Publikováno v:
Molecular and biochemical parasitology. 6(1)
Highly synchronized blood infections of Plasmodium chabaudi (strain IP-PCI) were induced in mice. Pulse-labelling in vitro experiments, using [ 35 S]methionine, identified 28 proteins whose synthesis is exclusively or preferentially observed during s