Continuous Process Verification 4.0 application in upstream: adaptiveness implementation managed by AI in the hypoxic bioprocess of the Pichia pastoris cell factory

Autor: Arnau Gasset, Joeri Van Wijngaarden, Ferran Mirabent, Albert Sales-Vallverdú, Xavier Garcia-Ortega, José Luis Montesinos-Seguí, Toni Manzano, Francisco Valero

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)

The experimental approach developed in this research demonstrated how the cloud, the Internet of Things (IoT), edge computing, and Artificial Intelligence (AI), considered key technologies in Industry 4.0, provide the expected horizon for adaptive vi

Externí odkaz: https://doaj.org/article/f5ebe78bac7040099055095e42d5b3fd

Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9

Autor: Javier Garrigós-Martínez, Astrid Weninger, José Luis Montesinos-Seguí, Christian Schmid, Francisco Valero, Claudia Rinnofner, Anton Glieder, Xavier Garcia-Ortega

Publikováno v: Microbial Cell Factories, Vol 20, Iss 1, Pp 1-13 (2021)

Abstract Background Currently, the numerous and versatile applications in pharmaceutical and chemical industry make the recombinant production of cytochrome P450 enzymes (CYPs) of great biotechnological interest. Accelerating the drug development pro

Externí odkaz: https://doaj.org/article/1f2f265f88be497a87493bbd47e289ac

Rationale‐based selection of optimal operating strategies and gene dosage impact on recombinant protein production in Komagataella phaffii (Pichia pastoris)

Autor: Miguel Angel Nieto‐Taype, Javier Garrigós‐Martínez, Marc Sánchez‐Farrando, Francisco Valero, Xavier Garcia‐Ortega, José Luis Montesinos‐Seguí

Publikováno v: Microbial Biotechnology, Vol 13, Iss 2, Pp 315-327 (2020)

Summary Its features as a microbial and eukaryotic organism have turned Komagataella phaffii (Pichia pastoris) into an emerging cell factory for recombinant protein production (RPP). As a key step of the bioprocess development, this work aimed to dem

Externí odkaz: https://doaj.org/article/050690a9c4c84d34bdda4d42ed84edcb

Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) P AOX1 -driven recombinant producer strains with different target gene dosage

Autor: Javier Garrigós-Martínez, Miguel Angel Nieto-Taype, Arnau Gasset-Franch, José Luis Montesinos-Seguí, Xavier Garcia-Ortega, Francisco Valero

Publikováno v: Microbial Cell Factories, Vol 18, Iss 1, Pp 1-15 (2019)

Abstract Background The P AOX1 -based expression system is the most widely used for producing recombinant proteins in the methylotrophic yeast Pichia pastoris (Komagataella phaffii). Despite relevant recent advances in regulation of the methanol util

Externí odkaz: https://doaj.org/article/74bc68089f074e718ee6a9073c01ca84

Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris

Autor: Ilya I. Tolstorukov, Miguel Angel Nieto-Taype, Francisco Valero, Merja Penttilä, Xavier Garcia-Ortega, Christian Schmid, José Luis Montesinos-Seguí, Knut Madden, Juha Tähtiharju, Kiira Vuoristo, Anton Glieder, Jaana Uusitalo, Pauliina Tukiainen, Javier Garrigós-Martínez

Publikováno v: Garrigós-Martínez, J, Vuoristo, K, Nieto-Taype, M A, Tähtiharju, J, Uusitalo, J, Tukiainen, P, Schmid, C, Tolstorukov, I, Madden, K, Penttilä, M, Montesinos-Seguí, J L, Valero, F, Glieder, A & Garcia-Ortega, X 2021, ' Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris ', Microbial Cell Factories, vol. 20, no. 1, 74 . https://doi.org/10.1186/s12934-021-01564-9
Universitat Autònoma de Barcelona
Microbial Cell Factories, Vol 20, Iss 1, Pp 1-12 (2021)
Dipòsit Digital de Documents de la UAB
Microbial Cell Factories

Background Pichia pastoris is a powerful and broadly used host for recombinant protein production (RPP), where past bioprocess performance has often been directed with the methanol regulated AOX1 promoter (PAOX1), and the constitutive GAP promoter (P

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2cc4373f7524246f16fe98260f683ce
https://cris.vtt.fi/en/publications/78573d8c-33b1-40f7-86f2-f4c89a4177c3