Zobrazeno 1 - 10
of 38
pro vyhledávání: '"José L. Moreira"'
Autor:
Hansjörg Hauser, A. V. Carvalhal, Paula M. Alves, José L. Moreira, Ana S. Coroadinha, Manuel J.T. Carrondo
Publikováno v:
Enzyme and Microbial Technology. 30:95-109
A genetic approach based on the activation of interferon-regulated-factor-1 (IRF-1) has been applied to arrest BHK cell growth in spinner flasks through the presence of estradiol in the culture medium, leading to the activation of the constitutively
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ba64818c21b5c5e92768d63983fb034d
https://doi.org/10.1016/s0141-0229(01)00460-4
https://doi.org/10.1016/s0141-0229(01)00460-4
Publikováno v:
Journal of Biotechnology. 92:47-59
Activation of the constitutively expressed interferon-regulatory-factor-1/estrogen receptor fusion protein (IRF-1-hER) in BHK cells was accomplished through the addition of estradiol to the culture medium, which enabled IRF-1 to gain its transcriptio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87b2fa4f9ccea24b2a0cecbba24ccb9b
https://doi.org/10.1016/s0168-1656(01)00365-0
https://doi.org/10.1016/s0168-1656(01)00365-0
Publikováno v:
Journal of Biotechnology. 80:109-118
The aim of this work was the optimisation of a fed-batch culture by metabolic confinement of BHK21 cells producing an antibody/cytokine fusion protein with potential application in tumour-targeted therapy. Previous results showed that at very low nut
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e740e3472860244b105ef27369b99ef9
https://doi.org/10.1016/s0168-1656(00)00254-6
https://doi.org/10.1016/s0168-1656(00)00254-6
Publikováno v:
Biotechnology and Bioengineering. 52:429-432
The influence of surface growth (two-dimensional microcarriers) and three-dimensional growth (aggregates and macroporous supports) in agitated, suspended batch culture systems upon growth and productivity of BHK was compared. Cultures using three por
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3feb46c8c85a07aadf85068fd801adfe
https://doi.org/10.1002/(sici)1097-0290(19961105)52:3<429::aid-bit9>3.0.co
https://doi.org/10.1002/(sici)1097-0290(19961105)52:3<429::aid-bit9>3.0.co
Autor:
Paula M. Alves, José L. Moreira, Manfred Nimtz, Manuel J.T. Carrondo, Cristina Peixoto, Helder J. Cruz, Elsa M. Dias
Publikováno v:
Biotechnology and Bioengineering. 69:129-139
BHK-21 cells expressing a human IgG-IL2 fusion protein, with potential application in tumor-targeted therapy, were grown under different nutrient conditions in a continuous system for a time period of 80 days. At very low-glucose (< 0.5 mM) or glutam
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::575cc1f80a554e793986f6d5a12c12a0
https://doi.org/10.1002/(sici)1097-0290(20000720)69:2<129::aid-bit2>3.0.co
https://doi.org/10.1002/(sici)1097-0290(20000720)69:2<129::aid-bit2>3.0.co
Autor:
Peter P. Mueller, A. V. Carvalhal, José L. Moreira, Helder J. Cruz, Hansjörg Hauser, Manuel J.T. Carrondo
Publikováno v:
Cytotechnology. 32:135-145
The activation of interferon-regulatory-factor-1 (IRF-1) hasbeen applied to regulate the cell growth of BHK cells. Theconstitutively expressed IRF-1-estrogen receptor fusion protein(IRF-1-hER) activated by the addition to the culture medium ofan estr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1fe7bef1b52c61468d90942030e5cae7
https://doi.org/10.1023/a:1008139304964
https://doi.org/10.1023/a:1008139304964
Autor:
Kathleen Devos, Pedro E. Cruz, Manuel J.T. Carrondo, José L. Moreira, Cristina Peixoto, Eric Saman
Publikováno v:
Enzyme and Microbial Technology. 26:61-70
This work aimed at the characterisation and downstream processing of HIV-1 core and virus-like particles (CLPs and VLPs) produced in serum free medium. The produced core and virus-like particles have similar characteristics to those of the native imm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::03d8f07444a595e6004f978e0677e8f7
https://doi.org/10.1016/s0141-0229(99)00128-3
https://doi.org/10.1016/s0141-0229(99)00128-3
Autor:
Paula M. Alves, Pedro E. Cruz, José L. Moreira, Helena Santos, Manuel J.T. Carrondo, Pedro C. Martins, Cristina Peixoto
Publikováno v:
Biotechnology and Bioengineering. 65:133-143
In this work the proteolytic activity in the supernatant and inside insect cells in culture was evaluated for different multiplicities of infection (MOI) and times of infection (TOI). Several methods to detect proteolytic activity in insect cells wer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::528681ab69327b12c09c7cdb1249b3a8
https://doi.org/10.1002/(sici)1097-0290(19991020)65:2<133::aid-bit2>3.0.co
https://doi.org/10.1002/(sici)1097-0290(19991020)65:2<133::aid-bit2>3.0.co
Autor:
Glyn N. Stacey, A. V. Savage, M. Cuffe, C. Mielke, E. Rieke, J. B. Griffiths, T. Welge, C. Burger, D. Looby, José L. Moreira, Hansjörg Hauser, Manuel J.T. Carrondo, Elsa M. Dias, K. Hayes, Helder J. Cruz
Publikováno v:
Applied Microbiology and Biotechnology. 52:345-353
Recombinant fusion proteins offer important new therapeutic approaches for the future. This report describes the use of three different genetic strategies (i.e. “mono-”, “bi-” and “tri-cistronic” vectors) to achieve stable secretion from
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::426b08f2f29b6c2547400979caf05f3e
https://doi.org/10.1007/s002530051530
https://doi.org/10.1007/s002530051530
Publikováno v:
Applied Microbiology and Biotechnology. 51:579-585
In this work, a BHK21 clone producing a recombinant antibody/cytokine fusion protein was used to study the dependence of cell metabolism on the glucose and glutamine levels in the culture medium. Results obtained indicate that both glucose and glutam
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3157a6df37f3d6f9af485ff0a7d73ad
https://doi.org/10.1007/s002530051435
https://doi.org/10.1007/s002530051435