Výsledky vyhledávání - "Jorrit W. Hegge"

Akademický článek

Argonaute bypasses cellular obstacles without hindrance during target search

Autor: Tao Ju Cui, Misha Klein, Jorrit W. Hegge, Stanley D. Chandradoss, John van der Oost, Martin Depken, Chirlmin Joo

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019)

Argonaute protein is loaded with small RNA and scans long stretch of sequences to find complementary target sites. Here, using single-molecule FRET and kinetic modelling, the authors showed that prokaryotic Argonaute protein binds target DNA loosely

Externí odkaz: https://doaj.org/article/030bdb4c6a084d029bfa423bfee6d58d

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High-Speed Super-Resolution Imaging Using Protein-Assisted DNA-PAINT

Autor: Mike Filius, Adithya N. Ananth, Chirlmin Joo, Tao Ju Cui, John van der Oost, M.W. Docter, Jorrit W. Hegge

Publikováno v: Nano Letters
Nano letters, 4, 20, 2264-2270
Nano Letters 20 (2020) 4
Nano Letters, 20(4), 2264-2270
Nano Letters: a journal dedicated to nanoscience and nanotechnology, 20(4)

Super-resolution imaging allows for visualization of cellular structures on a nanoscale level. DNA-PAINT (DNA Point Accumulation In Nanoscale Topology) is a super-resolution method that depends on the binding and unbinding of DNA imager strands. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6519dd5152cd5d1aabb2e84a05256d2
http://europepmc.org/articles/PMC7146856

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CRISPR-Clear: A Fieldable Detection Procedure for Potential CRISPR-Cas9 Gene Drive Based Bioweapons

Autor: Vittorio Saggiomo, Aldrik Velders, Jorrit W. Hegge, Angelina Horsting, Martijn M. van Galen, Anna C. Nieuwenweg

Rapid progression in genetic modification research has made gene editing increasingly cheaper and easier to use. CRISPR-Cas9 for example, allows for the specific alteration of the genome of an organism with relative simplicity and low costs. This rai

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::32681291e61fccd52790632b9c7245f2
https://doi.org/10.26434/chemrxiv.7756097

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Argonaute bypasses cellular obstacles without hindrance during target search

Autor: Jorrit W. Hegge, Tao Ju Cui, John van der Oost, Stanley D. Chandradoss, Chirlmin Joo, Misha Klein, Martin Depken

Publikováno v: Nature Communications, 10(1)
Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
Nature Communications 10 (2019)
Nature Communications
Nature Communications, 10

Argonaute (Ago) proteins are key players in both gene regulation (eukaryotes) and host defense (prokaryotes). Acting on single-stranded nucleic-acid substrates, Ago relies on base pairing between a small nucleic-acid guide and its complementary targe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1de4f7fde0c64c2ce99e4d2c1be55696
http://resolver.tudelft.nl/uuid:a82e91c9-9112-49a5-8758-6f4a6616292a

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DNA-guided DNA cleavage at moderate temperatures by Clostridium butyricum Argonaute

Autor: Jorrit W. Hegge, Tao Ju Cui, Daan C. Swarts, Stanley D. Chandradoss, Chirlmin Joo, Martin Jinek, Jeroen Kneppers, John van der Oost

Publikováno v: Nucleic acids research, 47(11), 5809-5821
Nucleic Acids Research
Nucleic acids research, 47(11)
Nucleic acids research 47 (2019) 11

Prokaryotic Argonaute proteins (pAgos) constitute a diverse group of endonucleases of which some mediate host defense by utilizing small interfering DNA guides (siDNA) to cleave complementary invading DNA. This activity can be repurposed for programm

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b11d525155503433f6481ba5aec0ed88
https://research.wur.nl/en/publications/dna-guided-dna-cleavage-at-moderate-temperatures-by-clostridium-b

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Highly specific enrichment of rare nucleic acids usingThermus thermophilusArgonaute

Autor: Erica L. Carpenter, Lotte T. Azink, Michael G. Mauk, Jorrit W. Hegge, Moen Sen, John van der Oost, Junman Chen, Neha Bhagwat, Jazmine Mays, Jing Peng, Jacob Till, Haim H. Bau, Jinzhao Song

Detection of disease-associated, cell-free nucleic acids enables early diagnostics, genotyping, and personalized therapy, but is challenged by their low concentration and sequence homology with abundant wild-type nucleic acids. We describe a novel ap

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9493e3123579bd3108043f5e56564240
https://doi.org/10.1101/491738

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Highly specific enrichment of rare nucleic acid fractions using Thermus thermophilus argonaute with applications in cancer diagnostics

Autor: Jorrit W. Hegge, Jazmine Mays, Moen Sen, Michael G. Mauk, Junman Chen, Jing Peng, Erica L. Carpenter, Jinzhao Song, Haim H. Bau, John van der Oost, Lotte T. Azink, Jacob Till, Neha Bhagwat

Publikováno v: Nucleic acids research 48 (2020) 4
Nucleic acids research, 48(4), e19-e19
Nucleic Acids Research

Detection of disease-associated, cell-free nucleic acids in body fluids enables early diagnostics, genotyping and personalized therapy, but is challenged by the low concentrations of clinically significant nucleic acids and their sequence homology wi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8496373e8e219f4e4773751d01992ac8
https://doi.org/10.1093/nar/gkz1165

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Prokaryotic Argonaute proteins : novel genome-editing tools?

Autor: Jorrit W. Hegge, John van der Oost, Daan C. Swarts

Publikováno v: Nature Reviews Microbiology 16 (2018) 1
Nature Reviews Microbiology, 16(1), 5-11

Argonaute proteins constitute a highly diverse family of nucleic acid-guided proteins. They were first discovered in eukaryotes as key proteins in RNA interference systems, but homologous prokaryotic Argonaute proteins (pAgos) have also been found in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68508b055c006e7e59cf0ef3e397f677
https://research.wur.nl/en/publications/prokaryotic-argonaute-proteins-novel-genome-editing-tools

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Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA

Autor: Masami Shiimori, John van der Oost, Ismael Hinojo, Rebecca M. Terns, Jorrit W. Hegge, Michael P. Terns, Michael A. Ellis, Daan C. Swarts, Justin Dumrongkulraksa

Publikováno v: Nucleic acids research 43 (2015) 10
Nucleic acids research, 43(10), 5120-5129
Nucleic Acids Research

Functions of prokaryotic Argonautes (pAgo) have long remained elusive. Recently, Argonautes of the bacteria Rhodobacter sphaeroides and Thermus thermophilus were demonstrated to be involved in host defense. The Argonaute of the archaeon Pyrococcus fu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35ae2e3cb02d90783a504c221e28eb47
https://research.wur.nl/en/publications/argonaute-of-the-archaeon-pyrococcus-furiosus-is-a-dna-guided-nuc

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Argonaute Target Search is Facilitated by Long Distance Diffusion

Autor: Stanley D. Chandradoss, Jorrit W. Hegge, John van der Oost, Chirlmin Joo, Tao Ju Cui

Publikováno v: Biophysical Journal. 112:314a

Argonaute proteins have a variety of functions ranging from post transcriptional gene silencing in eukaryotes to host defence in prokaryotic systems. Despite the well-established functional understanding of Argonautes, the biophysical understanding o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2e106f6f6b0187aa5d2fd674cb264012
https://doi.org/10.1016/j.bpj.2016.11.1701

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