Optogenetic regulation of endogenous proteins

Autor: Taras A. Redchuk, Maksim M. Karasev, Polina V. Verkhusha, Sara K. Donnelly, Maren Hülsemann, Jori Virtanen, Henna M. Moore, Maria K. Vartiainen, Louis Hodgson, Vladislav V. Verkhusha

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)

Optogenetic approaches to control protein-protein interactions usually require overexpression of the target proteins. Here the authors integrate intrabodies into near-infrared- and blue-light activatable optogenetic tools to control endogenous protei

Externí odkaz: https://doaj.org/article/6f20a13284cc4e2a9ddb3638c37c57ca

Optogenetic regulation of endogenous proteins

Autor: Jori Virtanen, Maren Hülsemann, Polina V Verkhusha, Maria K. Vartiainen, Louis Hodgson, Vladislav V. Verkhusha, Henna M. Moore, Taras A. Redchuk, Sara K. Donnelly, Maksim M. Karasev

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Nature Communications

Techniques of protein regulation, such as conditional gene expression, RNA interference, knock-in and knock-out, lack sufficient spatiotemporal accuracy, while optogenetic tools suffer from non-physiological response due to overexpression artifacts.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69f27d34ad99881cfb912d72a71b52c9
http://hdl.handle.net/10138/317811

Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing

Autor: Markku Varjosalo, Salla Kyheröinen, Jori Virtanen, Tiina Viita, Maria K. Vartiainen, Bina Prajapati

In addition to its essential functions within the cytoskeleton, actin also localizes to the cell nucleus, where it is linked to many important nuclear processes from gene expression to maintenance of genomic integrity. However, the molecular mechanis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4dfae74c85eca724cb310405b9d3327a

Chromatin organization regulates viral egress dynamics

Autor: Elina Mäntylä, Markko Myllys, Keijo Mattila, Jussi Timonen, Visa Ruokolainen, Carolyn A. Larabell, Satu Hakanen, Thomas Kühn, Vesa Aho, Jori Virtanen, Maija Vihinen-Ranta, Veijo Hukkanen

Publikováno v: Scientific Reports
Scientific reports, vol 7, iss 1
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
Aho, V; Myllys, M; Ruokolainen, V; Hakanen, S; Mantyla, E; Virtanen, J; et al.(2017). Chromatin organization regulates viral egress dynamics. SCIENTIFIC REPORTS, 7. doi: 10.1038/s41598-017-03630-y. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/47s4z8h8

Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined wit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b1025818ee6f02ac656e4a776765db6
https://pubmed.ncbi.nlm.nih.gov/28623258