Differential sensing with arrays of de novo designed peptide assemblies

Autor: William M. Dawson, Kathryn L. Shelley, Jordan M. Fletcher, D. Arne Scott, Lucia Lombardi, Guto G. Rhys, Tania J. LaGambina, Ulrike Obst, Antony J. Burton, Jessica A. Cross, George Davies, Freddie J. O. Martin, Francis J. Wiseman, R. Leo Brady, David Tew, Christopher W. Wood, Derek N. Woolfson

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)

Differential sensing aims to mimic senses such as taste and smell through the use of synthetic receptors. Here, the authors show that arrays of de novo designed peptide assemblies can be used as sensor components to distinguish various analytes and c

Externí odkaz: https://doaj.org/article/a820f403adc549ffa2b3bf2e841354ca

De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments

Autor: Matthew J. Lee, Judith Mantell, Ian R. Brown, Jordan M. Fletcher, Paul Verkade, Richard W. Pickersgill, Derek N. Woolfson, Stefanie Frank, Martin J. Warren

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018)

Bacterial microcompartments (BMCs) are protein-bound organelles encapsulating segments of metabolic pathways. Here the authors utilize specific de novo coiled-coil protein-protein interactions to display proteins on the outer or inner surface of BMCs

Externí odkaz: https://doaj.org/article/c4588752a0774db5a65123c4b6546adb

Beyond icosahedral symmetry in packings of proteins in spherical shells

Autor: Deborah K. Shoemark, Derek N. Woolfson, Richard B. Sessions, Tanniemola B. Liverpool, Majid Mosayebi, Noah Linden, Jordan M. Fletcher

Publikováno v: Mosayebi, M, Shoemark, D K, Fletcher, J M, Sessions, R B, Linden, N, Woolfson, D N & Liverpool, T B 2017, ' Beyond icosahedral symmetry in packings of proteins in spherical shells ', Proceedings of the National Academy of Sciences of the United States of America, vol. 114, no. 34, pp. 9014-9019 . https://doi.org/10.1073/pnas.1706825114

The formation of quasi-spherical cages from protein building blocks is a remarkable self-assembly process in many natural systems, where a small number of elementary building blocks are assembled to build a highly symmetric icosahedral cage. In turn,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6227a95e738daa0f331ba12a8e45e51d
https://doi.org/10.1073/pnas.1706825114

De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments

Autor: Derek N. Woolfson, Judith Mantell, Ian R. Brown, Stefanie Frank, Jordan M. Fletcher, Richard W. Pickersgill, Matthew J. Lee, Martin J. Warren, Paul Verkade

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
Nature Communications
Lee, M J, Mantell, J, Brown, I R, Fletcher, J M, Verkade, P, Pickersgill, R W, Woolfson, D N, Frank, S & Warren, M J 2018, ' De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments ', Nature Communications, vol. 9, 3413 . https://doi.org/10.1038/s41467-018-05922-x

Bacterial microcompartments, BMCs, are proteinaceous organelles that encase a specific metabolic pathway within a semi-permeable protein shell. Short encapsulation peptides can direct cargo proteins to the lumen of the compartments. However, the fusi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5e55bdc3ae883c0355a709bde184107
https://doi.org/10.1038/s41467-018-05922-x

Bioinspired Silicification Reveals Structural Detail in Self-Assembled Peptide Cages

Autor: Guto G. Rhys, Wei-Feng Xue, Johanna M. Galloway, Robert L. Harniman, Joseph L. Beesley, Judith Mantell, Derek N. Woolfson, Majid Mosayebi, Tanniemola B. Liverpool, Lorna Hodgson, Paul Verkade, Noah Linden, Laura Senior, Jennifer Coombs, Jordan M. Fletcher, Paul Curnow

Publikováno v: Galloway, J M, Senior, L, Fletcher, J M, Beesley, J L, Hodgson, L R, Harniman, R L, Mantell, J M, Coombs, J, Rhys, G G, Xue, W-F, Mosayebi, M, Linden, N, Liverpool, T B, Curnow, P, Verkade, P & Woolfson, D N 2018, ' Bioinspired Silicification Reveals Structural Detail in Self-Assembled Peptide Cages ', ACS Nano, vol. 12, no. 2, pp. 1420–1432 . https://doi.org/10.1021/acsnano.7b07785
ACS Nano

Understanding how molecules in self-assembled soft-matter nanostructures are organized is essential for improving the design of next-generation nanomaterials. Imaging these assemblies can be challenging and usually requires processing, e.g. staining

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d4adb2ef9dbc878edcc93d35427cb2d
https://hdl.handle.net/1983/597ff710-325f-4d82-ac3f-003afee7dba4