Zobrazeno 1 - 10
of 92
pro vyhledávání: '"Joop Van den Heuvel"'
Autor:
Maeva Katzmarzyk, Denise Christine Clesle, Joop van den Heuvel, Markus Hoffmann, Henk Garritsen, Stefan Pöhlmann, Henning Jacobsen, Luka Čičin-Šain
Publikováno v:
Frontiers in Immunology, Vol 14 (2023)
IntroductionThe evolution of novel SARS-CoV-2 variants significantly affects vaccine effectiveness. While these effects can only be studied retrospectively, neutralizing antibody titers are most used as correlates of protection. However, studies asse
Externí odkaz:
https://doaj.org/article/0d555094971546a5b1a66768eead924e
Publikováno v:
Biomolecules, Vol 13, Iss 5, p 817 (2023)
Membrane proteins are difficult biomolecules to express and purify. In this paper, we compare the small-scale production of six selected eukaryotic integral membrane proteins in insect and mammalian cell expression systems using different techniques
Externí odkaz:
https://doaj.org/article/799bcd013a06411a8c7b4e244f48ea28
Autor:
Janin Korn, Dorina Schäckermann, Toni Kirmann, Federico Bertoglio, Stephan Steinke, Janyn Heisig, Maximilian Ruschig, Gertrudis Rojas, Nora Langreder, Esther Veronika Wenzel, Kristian Daniel Ralph Roth, Marlies Becker, Doris Meier, Joop van den Heuvel, Michael Hust, Stefan Dübel, Maren Schubert
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
Abstract Antibodies are essential tools for therapy and diagnostics. Yet, production remains expensive as it is mostly done in mammalian expression systems. As most therapeutic IgG require mammalian glycosylation to interact with the human immune sys
Externí odkaz:
https://doaj.org/article/58d50e96dc5f45bcb3f271b3937cc6e0
Autor:
Marcel Jaron, Michael Lehky, Marta Zarà, Chris Nicole Zaydowicz, Aidin Lak, Rico Ballmann, Philip Alexander Heine, Esther Veronika Wenzel, Kai-Thomas Schneider, Federico Bertoglio, Susanne Kempter, Reinhard Wolfgang Köster, Silvia Stella Barbieri, Joop van den Heuvel, Michael Hust, Stefan Dübel, Maren Schubert
Publikováno v:
Viruses, Vol 14, Iss 10, p 2087 (2022)
Virus-like particles (VLPs) resemble authentic virus while not containing any genomic information. Here, we present a fast and powerful method for the production of SARS-CoV-2 VLP in insect cells and the application of these VLPs to evaluate the inhi
Externí odkaz:
https://doaj.org/article/059ba81e8dae4b76b6f823fda47e7e79
Autor:
Federico Bertoglio, Viola Fühner, Maximilian Ruschig, Philip Alexander Heine, Leila Abassi, Thomas Klünemann, Ulfert Rand, Doris Meier, Nora Langreder, Stephan Steinke, Rico Ballmann, Kai-Thomas Schneider, Kristian Daniel Ralph Roth, Philipp Kuhn, Peggy Riese, Dorina Schäckermann, Janin Korn, Allan Koch, M. Zeeshan Chaudhry, Kathrin Eschke, Yeonsu Kim, Susanne Zock-Emmenthal, Marlies Becker, Margitta Scholz, Gustavo Marçal Schmidt Garcia Moreira, Esther Veronika Wenzel, Giulio Russo, Hendrikus S.P. Garritsen, Sebastian Casu, Andreas Gerstner, Günter Roth, Julia Adler, Jakob Trimpert, Andreas Hermann, Thomas Schirrmann, Stefan Dübel, André Frenzel, Joop Van den Heuvel, Luka Čičin-Šain, Maren Schubert, Michael Hust
Publikováno v:
Cell Reports, Vol 36, Iss 4, Pp 109433- (2021)
Summary: The novel betacoronavirus severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) causes a form of severe pneumonia disease called coronavirus disease 2019 (COVID-19). To develop human neutralizing anti-SARS-CoV-2 antibodies, antibody g
Externí odkaz:
https://doaj.org/article/9c2876f60a3849cc9e53afa0c2d3362e
Autor:
Marc Sevenich, Elena Thul, Nils-Alexander Lakomek, Thomas Klünemann, Maren Schubert, Federico Bertoglio, Joop van den Heuvel, Patrick Petzsch, Jeannine Mohrlüder, Dieter Willbold
Publikováno v:
Biomedicines, Vol 10, Iss 2, p 441 (2022)
Severe respiratory syndrome coronavirus-2 (SARS-CoV-2) is a highly contagious beta-class coronavirus. Although vaccinations have shown high efficacy, the emergence of novel variants of concern (VOCs) has already exhibited traits of immune evasion. Th
Externí odkaz:
https://doaj.org/article/a30d7e10cf784031bebef2c32e46f490
Autor:
Danielle M. DiCara, Dimitri Y. Chirgadze, Anthony R. Pope, Aneesh Karatt-Vellatt, Anja Winter, Peter Slavny, Joop van den Heuvel, Kothai Parthiban, Jane Holland, Len C. Packman, Georgia Mavria, Jens Hoffmann, Walter Birchmeier, Ermanno Gherardi, John McCafferty
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
Abstract The growth and motility factor Hepatocyte Growth Factor/Scatter Factor (HGF/SF) and its receptor, the product of the MET proto-oncogene, promote invasion and metastasis of tumor cells and have been considered potential targets for cancer the
Externí odkaz:
https://doaj.org/article/e94db5aa07fa4eeeb3f23e45f09d95df
Autor:
Maren Bleckmann, Margitta Schürig, Michelle Endres, Anke Samuels, Daniela Gebauer, Nadine Konisch, Joop van den Heuvel
Publikováno v:
PLoS ONE, Vol 14, Iss 6, p e0217878 (2019)
Virus-free, transient gene expression (TGE) in High Five cells was recently presented as an efficient protein production method. However, published TGE protocols have not been standardized to a general protocol. Therefore, reproducibility and impleme
Externí odkaz:
https://doaj.org/article/1b306562bc9f4724a84365c5f73bb924
ER intrabody-mediated inhibition of interferon α secretion by mouse macrophages and dendritic cells.
Autor:
Konrad Büssow, Philipp Themann, Sabine Luu, Paul Pentrowski, Claudia Harting, Mira Majewski, Veith Vollmer, Mario Köster, Martina Grashoff, Rainer Zawatzky, Joop Van den Heuvel, Andrea Kröger, Thomas Böldicke
Publikováno v:
PLoS ONE, Vol 14, Iss 4, p e0215062 (2019)
Interferon α (IFNα) counteracts viral infections by activating various IFNα-stimulated genes (ISGs). These genes encode proteins that block viral transport into the host cell and inhibit viral replication, gene transcription and translation. Due t
Externí odkaz:
https://doaj.org/article/cbd17a8e11424f719b2c35a9f64189d1
Autor:
Yazh Muthukumar, Johanna Münkemer, Daniel Mathieu, Christian Richter, Harald Schwalbe, Heinrich Steinmetz, Wolfgang Kessler, Joachim Reichelt, Ulrike Beutling, Ronald Frank, Konrad Büssow, Joop van den Heuvel, Mark Brönstrup, Richard E Taylor, Sabine Laschat, Florenz Sasse
Publikováno v:
PLoS ONE, Vol 13, Iss 7, p e0201605 (2018)
The identification of inhibitors of eukaryotic protein biosynthesis, which are targeting single translation factors, is highly demanded. Here we report on a small molecule inhibitor, gephyronic acid, isolated from the myxobacterium Archangium gephyra
Externí odkaz:
https://doaj.org/article/773d2a0fde3942c3a38ead1de53be427