Zobrazeno 1 - 10
of 96
pro vyhledávání: '"Joon Shick Rhee"'
Publikováno v:
Enzyme and Microbial Technology. 39:1190-1196
The gene cluster encoding AprDEF, three-component ABC exporter apparatus of type I secretion pathway of Pseudomonas aeruginosa lipase, was coexpressed with the genomic DNA library of Erwinia chrysanthemi KCTC 2569 in Escherichia coli. Among about 150
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::292d49c5164102c26d51539c2d4fb5ec
https://doi.org/10.1016/j.enzmictec.2006.02.030
https://doi.org/10.1016/j.enzmictec.2006.02.030
Publikováno v:
Journal of the American Oil Chemists' Society. 82:691-705
Various kinds of phospholipids have wide industrial applications such as in food and nutraceuticals, cosmetics, agricultural products, and pharmaceuticals. The demand for reliable biocatalysts for the production of phospholipid products, such as phos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::86c4ac1b2a75132673742870fdda9da3
https://doi.org/10.1007/s11746-005-1131-0
https://doi.org/10.1007/s11746-005-1131-0
Autor:
Hiroyuki Tanaka, Young Nam Kwon, Gi-Hyuck Ryu, Sung-Ho Bae, Joon Shick Rhee, Do-Hyung Kim, Jeong-Hoon Kim, Stuart A. MacNeill, Yeon-Soo Seo
Publikováno v:
Nucleic Acids Research. 32:4205-4216
The Schizosaccharomyces pombe pfh1 + gene (PIF1 homolog) encodes an essential enzyme that has both DNA helicase and ATPase activities and is implicated in lagging strand DNA processing. Mutations in the pfh1 + gene suppress a temperature-sensitive al
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bcdb65cb1b036a094c387ce78ad47b1
https://doi.org/10.1093/nar/gkh720
https://doi.org/10.1093/nar/gkh720
Publikováno v:
Yeast. 21:437-443
Saccharomyces cerevisiae sphinganine C4-hydroxylase encoded by the SUR2 gene catalyses the conversion of sphinganine to phytosphingosine. We isolated the SUR2 gene from Pichia ciferrii using nucleotide sequence homology to S. cerevisiae SUR2 to study
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2b25ba34d7b0928049ae6639b707439
https://doi.org/10.1002/yea.1082
https://doi.org/10.1002/yea.1082
Publikováno v:
Applied and Environmental Microbiology. 68:6146-6151
A method of mutagenic and unidirectional reassembly (MURA) that can generate libraries of DNA-shuffled and randomly truncated proteins was developed. The method involved fragmenting the template gene(s) randomly by DNase I and reassembling the small
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c8ea63fa4577ca76282ecb2a3fb7631
https://doi.org/10.1128/aem.68.12.6146-6151.2002
https://doi.org/10.1128/aem.68.12.6146-6151.2002
Publikováno v:
Biotechnology Letters. 24:2113-2116
Lactobacillus delbrueckii subsp. delbrueckii KCTC 1047, grown in de Man, Rogosa and Sharpe (MRS) or soymilk media, completely hydrolyzed the isoflavone glucosides, genistin and daidzin at 50 μg ml−1, into their respective aglycones, genistein and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c0ccaa9d011d314e5b76d38ba25f177c
https://doi.org/10.1023/a:1021390120400
https://doi.org/10.1023/a:1021390120400
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 15:191-196
Enzymatic synthesis of 3-O-methyl-4-O-β- d -galactopyranosyl- d -glucose (3-O-methyl-lactose) has been attempted using both galactosyltransferase and galactosidase activities. The transferase-catalysed reaction produces exclusively the desired produ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ad35d85b23703022a8d06f5e73a07184
https://doi.org/10.1016/s1381-1177(01)00028-5
https://doi.org/10.1016/s1381-1177(01)00028-5
Autor:
Jae Kwang Song, Joon Shick Rhee
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1547:370-378
We attempted to apply the directed evolution approach to enhancing enzyme properties in the presence of organic solvents, in which enzyme stability and activity were often drastically reduced. Stability and catalytic activity of phospholipase A1 in t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1af6755d1ac11affef787ee3034fa142
https://doi.org/10.1016/s0167-4838(01)00204-7
https://doi.org/10.1016/s0167-4838(01)00204-7
Publikováno v:
Journal of the American Oil Chemists' Society. 78:291-296
For the synthesis of low-calorie structured lipids (LCSL), transesterification between triacetin and stearic acid using immobilized lipase in a solvent-free system was investigated. Stearic acid, a long-chain saturated fatty acid, was incorporated ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::de051cadb5336bef48a8532218c645f5
https://doi.org/10.1007/s11746-001-0259-2
https://doi.org/10.1007/s11746-001-0259-2
Autor:
Jae Kwang Song, Joon Shick Rhee
Publikováno v:
Applied and Environmental Microbiology. 66:890-894
The thermal stability and catalytic activity of phospholipase A 1 from Serratia sp. strain MK1 were improved by evolutionary molecular engineering. Two thermostable mutants were isolated after sequential rounds of error-prone PCR performed to introdu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02f5e04ac3f90a366c737ca7044c15cd
https://doi.org/10.1128/aem.66.3.890-894.2000
https://doi.org/10.1128/aem.66.3.890-894.2000