Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Jonathan W. Chin"'
Autor:
Robert J. Pantazes, George A. Khoury, Jonathan W. Chin, Costas D. Maranas, Hossein Fazelinia, Patrick C. Cirino
Publikováno v:
Protein Science. 18:2125-2138
In this study we introduce a computationally-driven enzyme redesign workflow for altering cofactor specificity from NADPH to NADH. By compiling and comparing data from previous studies involving cofactor switching mutations, we show that their effect
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21d98e3e9a7ea3d8875783bc44e1a194
https://doi.org/10.1002/pro.227
https://doi.org/10.1002/pro.227
Publikováno v:
Journal of Biotechnology. 134:246-252
Escherichia coli W3110 was previously engineered to co-utilize glucose and xylose by replacing the wild-type crp gene with a crp* mutant encoding a cAMP-independent CRP variant (Cirino et al., 2006 [Cirino, P.C., Chin, J.W., Ingram, L.O., 2006. Engin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfc57bcccbec2cdfd3cfc85e29032c7c
https://doi.org/10.1016/j.jbiotec.2008.02.003
https://doi.org/10.1016/j.jbiotec.2008.02.003
Publikováno v:
Biotechnology and Bioengineering. 95:1167-1176
The range of value-added chemicals produced by Escherichia coli from simple sugars has been expanded to include xylitol. This was accomplished by screening the in vivo activity of a number of heterologous xylitol-producing enzymes. Xylose reductases
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::161be34eed631254b17f6948d4b87a35
https://doi.org/10.1002/bit.21082
https://doi.org/10.1002/bit.21082
Autor:
K. Eng, Srdjan S. Nedeljkovic, Carlyle Hamsher, Rajivan Maniam, Natacha Zamor, Xiaodong Bao, Mark J Young, Darin J. Correll, Jose Zeballos, Johanna S. Ledley, Doo H. Lee, Jessica Sorace, Becky Tsui, Sunyoung Cho, Jonathan W Chin, Y. Zhang
Publikováno v:
BMJ Open
Introduction In spite of advances in understanding and technology, postoperative pain remains poorly treated for a significant number of patients. In colorectal surgery, the need for developing novel analgesics is especially important. Patients after
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c78e77ce085d9911d81f4e899340ee3
https://doi.org/10.1136/bmjopen-2016-011035
https://doi.org/10.1136/bmjopen-2016-011035
Autor:
Jonathan W, Chin, Patrick C, Cirino
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 743
This chapter provides an overview of key tools and methodologies available to practitioners of biocatalysis interested in using microorganisms to carry out biotransformations and describes specific examples of applying genetic modification strategies
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::83bd2373512d534a851e1417c0b22eb6
https://pubmed.ncbi.nlm.nih.gov/21553192
https://pubmed.ncbi.nlm.nih.gov/21553192
Autor:
Patrick C. Cirino, Jonathan W. Chin
Publikováno v:
Methods in Molecular Biology ISBN: 9781617791314
This chapter provides an overview of key tools and methodologies available to practitioners of biocatalysis interested in using microorganisms to carry out biotransformations and describes specific examples of applying genetic modification strategies
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7d41a798ceebbd199742e9acf3ac8580
https://doi.org/10.1007/978-1-61779-132-1_15
https://doi.org/10.1007/978-1-61779-132-1_15
Autor:
Patrick C. Cirino, Jonathan W. Chin
Publikováno v:
Biotechnology progress. 27(2)
Escherichia coli engineered to uptake xylose while metabolizing glucose was previously shown to produce high levels of xylitol from a mixture of glucose and xylose when expressing NADPH-dependent xylose reductase from Candida boidinii (CbXR) (Cirino
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ef732bbd7adc0a3d65a6dee8a7d490b
https://pubmed.ncbi.nlm.nih.gov/21344680
https://pubmed.ncbi.nlm.nih.gov/21344680
Publikováno v:
Journal of Biological Engineering
Journal of Biological Engineering, Vol 3, Iss 1, p 13 (2009)
Journal of Biological Engineering, Vol 3, Iss 1, p 13 (2009)
Background Escherichia coli exhibits diauxic growth in sugar mixtures due to CRP-mediated catabolite repression and inducer exclusion related to phosphotransferase system enzyme activity. Replacement of the native crp gene with a catabolite repressio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aba6b16dad458b40226552b4881b58e7
https://doi.org/10.1186/1754-1611-3-13
https://doi.org/10.1186/1754-1611-3-13
Publikováno v:
Biotechnology and bioengineering. 102(1)
Escherichia coli strain PC09 (DxylB, cAMP- independent CRP (crp � ) mutant) expressing an NADPH- dependent xylose reductase from Candida boidinii (CbXR) was previously reported to produce xylitol from xylose while metabolizing glucose (Cirino et al
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c9308e39330e776810dc9f5778482ba
https://pubmed.ncbi.nlm.nih.gov/18698648
https://pubmed.ncbi.nlm.nih.gov/18698648
Publikováno v:
Biotechnology letters. 30(9)
Escherichia coli W3110 was previously engineered to produce xylitol from a mixture of glucose plus xylose by expressing xylose reductase (CbXR) and deleting xylulokinase (DeltaxylB), combined with either plasmid-based expression of a xylose transport
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dfcd34d64e980425c50df4d655c1a98
https://pubmed.ncbi.nlm.nih.gov/18414795
https://pubmed.ncbi.nlm.nih.gov/18414795