Výsledky vyhledávání - "Jonathan W. Larson"

Microdroplet-based PCR enrichment for large-scale targeted sequencing

Autor: Masakazu Nakano, Kelly A. Frazer, Michael L. Samuels, Jonathan W. Larson, Martina Medkova, Eric J. Topol, J. Brian Hutchison, Brian Libby, Ryan Tewhey, Darren R. Link, Olivier Harismendy, Jeff Olson, Michael P. Weiner, Jason Warner, Patricia H David, Steve K. Kotsopoulos

Publikováno v: Nature biotechnology

Targeted sequencing of specific loci of the human genome is a promising approach for maximizing the efficiency of second-generation sequencing technologies for population-based studies of genetic variation. Here we describe microdroplet PCR, which pe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34a2a82634926e9d0e7bd64901ab1e7a
https://doi.org/10.1038/nbt.1583

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Application of single molecule technology to rapidly map long DNA and study the conformation of stretched DNA

Autor: Kimberly A. Gillis, Michael Gallo, Gregory R. Yantz, Steven R. Gullans, Nuno Goncalves, Christina M. D’Antoni, Rudolf Gilmanshin, Kevin M. Phillips, Lori Anne Neely, Jonathan W. Larson

Publikováno v: Nucleic Acids Research

Herein we describe the first application of direct linear analysis (DLA) to the mapping of a bacterial artificial chromosome (BAC), specifically the 185.1 kb-long BAC 12M9. DLA is a single molecule mapping technology, based on microfluidic elongation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa398ab7e877b26950b6c3e83bc301bd
https://doi.org/10.1093/nar/gki895

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DNA Mapping Using Microfluidic Stretching and Single-Molecule Detection of Fluorescent Site-Specific Tags

Autor: Nuno Goncalves, Eugene Y. Chan, Amie Jo Hatch, Steven R. Gullans, Eugene D. Carstea, Gordon G. Wong, Rudolf Gilmanshin, Jonathan W. Larson, Anthony M. Maletta, Rebecca A. Haeusler, Gregory R. Yantz, Martin Fuchs

Publikováno v: Genome Research. 14:1137-1146

We have developed a rapid molecular mapping technology—Direct Linear Analysis (DLA)—on the basis of the analysis of individual DNA molecules bound with sequence-specific fluorescent tags. The apparatus includes a microfluidic device for stretchin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::151e110fbbd8cb0d08140bdb18bbffde
https://doi.org/10.1101/gr.1635204

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Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR

Autor: Valérie Taly, Jeffrey L. Olson, Darren R. Link, Steve K. Kotsopoulos, Smiti Bhattacharya, Andrew D. Griffiths, Qun Zhong, Jonathan W. Larson

Publikováno v: Lab on a Chip
Lab on a Chip, Royal Society of Chemistry, 2011, 11 (13), pp.2167. ⟨10.1039/c1lc20126c⟩
Lab on a Chip, 2011, 11 (13), pp.2167. ⟨10.1039/c1lc20126c⟩

International audience; Quantitative polymerase chain reactions (qPCR) based on real-time PCR constitute a powerful and sensitive method for the analysis of nucleic acids. However, in qPCR, the ability to multiplex targets using differently colored f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3066b4ee433c370c3ae1110793ecedba
https://pubmed.ncbi.nlm.nih.gov/21584334

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A lab-on-chip for biothreat detection using single-molecule DNA mapping

Autor: Jeffrey R. Krogmeier, Bryan Crane, Griffis Joshua, Lisa W. Kwok, Rudolf Gilmanshin, Nanor Kojanian, Michelle K. Nahas, Qun Zhong, Vyacheslav Papkov, Saad Shaikh, Richard Allen, Gene Malkin, Kedar Vyavahare, Robert H. Meltzer, Jonathan W. Larson, Yi Zhou, Linda Knaian

Publikováno v: Lab on a chip. 11(5)

Rapid, specific, and sensitive detection of airborne bacteria, viruses, and toxins is critical for biodefense, yet the diverse nature of the threats poses a challenge for integrated surveillance, as each class of pathogens typically requires differen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7225b18afc68c0c7d8513bad723047fc
https://pubmed.ncbi.nlm.nih.gov/21249264

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A Microfluidic Device For Concentrating High Molecular Weight DNA

Autor: Bryan Crane, Rudolf Gilmanshin, Nanor Kojanian, Nicaulas Sabourin, Richard Allen, Yi Zhou, Jeffrey R. Krogmeier, Qun Zhong, Kedar Vyavahare, Saad Shaikh, Kate Carson, Jonathan W. Larson, Linda Knaian

Publikováno v: Biophysical Journal. 96(3)

Direct Linear Analysis (DLA) technology obtains high content sequence information by optically mapping sequence specific fluorescent tags bound to elongated genomic DNA molecules in shear flows.[1] To facilitate sensitivity and throughput, we have im

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3844111dc062c822dd088458d1009a9

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An Integrated Multifunctional Lab-on-a-Chip Platform for High Throughput Optical Mapping of DNA

Autor: Jeffrey R. Krogmeier, Richard Allen, Qun Zhong, Yi Zhou, Griffis Joshua, Wolf Mesadieu, Kedar Vyavahare, Lisa W. Kwok, Jonathan W. Larson, Rudolf Gilmanshin, Amanda Edmonson, Linda Knaian, Bryan Crane, Robert H. Meltzer

Publikováno v: Biophysical Journal. 96(3)

Direct Linear Analysis (DLA) technology employs continuous microfluidic elongational flow to stretch and optically map individual DNA molecules. Sequence specific tagging of DNA molecules with bisPNAs enables genomic differentiation between species.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::032545b1d788fa94374e5f6b611e7cf3

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An integrated optics microfluidic device for detecting single DNA molecules

Autor: Jonathan W. Larson, Jeffrey R. Krogmeier, George Seward, Ian Schaefer, Gregory R. Yantz

Publikováno v: Lab on a chip. 7(12)

A fluorescence-based integrated optics microfluidic device is presented, capable of detecting single DNA molecules in a high throughput and reproducible manner. The device integrates microfluidics for DNA stretching with two optical elements for sing

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62cd913fcd41edc2391bb1ea73299bbe
https://pubmed.ncbi.nlm.nih.gov/18030399

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Single DNA molecule stretching in sudden mixed shear and elongational microflows

Autor: Rebecca Charnas, Steven R. Gullans, Gregory R. Yantz, Kimberly A. Gillis, Gordon G. Wong, Rudolf Gilmanshin, Lori Anne Neely, Michael Gallo, Qun Zhong, Jonathan W. Larson, Christina M. D’Antoni, Kevin M. Phillips

Publikováno v: Lab on a chip. 6(9)

High-throughput stretching and monitoring of single DNA molecules in continuous elongational flow offers compelling advantages for biotechnology applications such as DNA mapping. However, the polymer dynamics in common microfluidic implementations ar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a11121ca01ae3b679e3d7e6586a8e85
https://pubmed.ncbi.nlm.nih.gov/16929398

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Preferential binding of equine ferricytochrome c to the bacterial photosynthetic reaction center from Rhodobacter sphaeroides

Autor: Colin A. Wraight, Jonathan W. Larson

Publikováno v: Biochemistry. 39(48)

Redox titration of horse heart cytochrome c (cyt c), in the presence of varying concentrations of detergent-solubilized photosynthetic reaction center (RC) from Rhodobacter sphaeroides, revealed an RC concentration-dependent decrease in the measured

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf740fe8a5879e2b28683f0f8faa3da7
https://pubmed.ncbi.nlm.nih.gov/11101298

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