Zobrazeno 1 - 10
of 423
pro vyhledávání: '"Jonathan S, Weissman"'
Autor:
Alina Guna, Katharine R. Page, Joseph M. Replogle, Theodore K. Esantsi, Maxine L. Wang, Jonathan S. Weissman, Rebecca M. Voorhees
Publikováno v:
BMC Genomics, Vol 24, Iss 1, Pp 1-13 (2023)
Abstract Mapping genetic interactions is essential for determining gene function and defining novel biological pathways. We report a simple to use CRISPR interference (CRISPRi) based platform, compatible with Fluorescence Activated Cell Sorting (FACS
Externí odkaz:
https://doaj.org/article/595c5032f9dd4a8ca48b4d5775c55fa0
Autor:
Sara Sunshine, Andreas S. Puschnik, Joseph M. Replogle, Matthew T. Laurie, Jamin Liu, Beth Shoshana Zha, James K. Nuñez, Janie R. Byrum, Aidan H. McMorrow, Matthew B. Frieman, Juliane Winkler, Xiaojie Qiu, Oren S. Rosenberg, Manuel D. Leonetti, Chun Jimmie Ye, Jonathan S. Weissman, Joseph L. DeRisi, Marco Y. Hein
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-13 (2023)
Abstract Genomic and proteomic screens have identified numerous host factors of SARS-CoV-2, but efficient delineation of their molecular roles during infection remains a challenge. Here we use Perturb-seq, combining genetic perturbations with a singl
Externí odkaz:
https://doaj.org/article/117592a52159409a97218fac481cd781
Autor:
Jacqueline Morales, David V. Allegakoen, José A. Garcia, Kristen Kwong, Pushpendra K. Sahu, Drew A. Fajardo, Yue Pan, Max A. Horlbeck, Jonathan S. Weissman, W. Clay Gustafson, Trever G. Bivona, Amit J. Sabnis
Publikováno v:
JCI Insight, Vol 7, Iss 23 (2022)
Oncogenic FOXO1 gene fusions drive a subset of rhabdomyosarcoma (RMS) with poor survival; to date, these cancer drivers are therapeutically intractable. To identify new therapies for this disease, we undertook an isogenic CRISPR-interference screen t
Externí odkaz:
https://doaj.org/article/07f18a49460b42de8d0814e3d0166e10
Autor:
Joseph M Replogle, Jessica L Bonnar, Angela N Pogson, Christina R Liem, Nolan K Maier, Yufang Ding, Baylee J Russell, Xingren Wang, Kun Leng, Alina Guna, Thomas M Norman, Ryan A Pak, Daniel M Ramos, Michael E Ward, Luke A Gilbert, Martin Kampmann, Jonathan S Weissman, Marco Jost
Publikováno v:
eLife, Vol 11 (2022)
CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions in cell biol
Externí odkaz:
https://doaj.org/article/af03cd98b4f34c128b98cd963fa2ba56
Autor:
Matthew G Jones, Alex Khodaverdian, Jeffrey J Quinn, Michelle M Chan, Jeffrey A Hussmann, Robert Wang, Chenling Xu, Jonathan S Weissman, Nir Yosef
Publikováno v:
Genome Biology, Vol 21, Iss 1, Pp 1-27 (2020)
Abstract The pairing of CRISPR/Cas9-based gene editing with massively parallel single-cell readouts now enables large-scale lineage tracing. However, the rapid growth in complexity of data from these assays has outpaced our ability to accurately infe
Externí odkaz:
https://doaj.org/article/3a8f548f7505481089047c053016429e
CRISPRi-based radiation modifier screen identifies long non-coding RNA therapeutic targets in glioma
Autor:
S. John Liu, Martina Malatesta, Brian V. Lien, Parna Saha, Shivani S. Thombare, Sung Jun Hong, Leslie Pedraza, Mark Koontz, Kyounghee Seo, Max A. Horlbeck, Daniel He, Harjus S. Birk, Miten Jain, Hugh E. Olsen, Mark Akeson, Jonathan S. Weissman, Michelle Monje, Nalin Gupta, David R. Raleigh, Erik M. Ullian, Daniel A. Lim
Publikováno v:
Genome Biology, Vol 21, Iss 1, Pp 1-18 (2020)
Abstract Background Long non-coding RNAs (lncRNAs) exhibit highly cell type-specific expression and function, making this class of transcript attractive for targeted cancer therapy. However, the vast majority of lncRNAs have not been tested as potent
Externí odkaz:
https://doaj.org/article/158146c58ea3458da92657b2d5201345
Autor:
Robert Coukos, David Yao, Mateo I Sanchez, Eric T Strand, Meagan E Olive, Namrata D Udeshi, Jonathan S Weissman, Steven A Carr, Michael C Bassik, Alice Y Ting
Publikováno v:
eLife, Vol 10 (2021)
The trafficking of specific protein cohorts to correct subcellular locations at correct times is essential for every signaling and regulatory process in biology. Gene perturbation screens could provide a powerful approach to probe the molecular mecha
Externí odkaz:
https://doaj.org/article/6d814cb3a91342cea81c16b1402d48aa
Publikováno v:
Annual Review of Cancer Biology. 7:111-129
During tumor evolution, cancer cells can acquire the ability to proliferate, invade neighboring tissues, evade the immune system, and spread systemically. Tracking this process remains challenging, as many key events occur stochastically and over lon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dcbde07f27c9cd73f0ab06efa8c849d0
https://doi.org/10.1146/annurev-cancerbio-061421-123301
https://doi.org/10.1146/annurev-cancerbio-061421-123301
Autor:
Maxence Le Vasseur, Jonathan Friedman, Marco Jost, Jiawei Xu, Justin Yamada, Martin Kampmann, Max A Horlbeck, Michelle R Salemi, Brett S Phinney, Jonathan S Weissman, Jodi Nunnari
Publikováno v:
eLife, Vol 10 (2021)
Dysfunction of the mitochondrial electron transport chain (mETC) is a major cause of human mitochondrial diseases. To identify determinants of mETC function, we screened a genome-wide human CRISPRi library under oxidative metabolic conditions with se
Externí odkaz:
https://doaj.org/article/cf05516692bc4a05a17589a4c4200ad1
Autor:
Marco Jost, Amy N Jacobson, Jeffrey A Hussmann, Giana Cirolia, Michael A Fischbach, Jonathan S Weissman
Publikováno v:
eLife, Vol 10 (2021)
Dendritic cells (DCs) regulate processes ranging from antitumor and antiviral immunity to host-microbe communication at mucosal surfaces. It remains difficult, however, to genetically manipulate human DCs, limiting our ability to probe how DCs elicit
Externí odkaz:
https://doaj.org/article/af2eea39e034442491abb1656b561b49