Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Jonathan R. Moll"'
Publikováno v:
ACS Chemical Neuroscience. 8:1213-1221
Immunoassays such as enzyme-linked immunosorbent assays (ELISAs) are widely used for diagnostics; however, antibodies as detection reagents may be insufficiently selective and have other shortcomings. We present a novel non-antibody-based detection m
Autor:
Evgenia G. Matveeva, Jonathan R. Moll, Hamid Charkhkar, Nathalia Peixoto, Joseph J. Pancrazio, Richard O. Cliff, Daniel G. McHail, Susheela Meyyappan
Publikováno v:
Brain research. 1629
In vitro assays offer a means of screening potential therapeutics and accelerating the drug development process. Here, we utilized neuronal cultures on planar microelectrode arrays (MEA) as a functional assay to assess the neurotoxicity of amyloid-β
Publikováno v:
Journal of Structural Biology. 155:130-139
B-ZIP transcription factors dimerization is mediated by a parallel coiled-coil termed the leucine zipper. We have evaluated the dimerization specificity of the seven coiled-coil B-ZIP proteins (ATF6, XBP, LZIP, NFIL3, TEF, CREB, and C/EBPalpha) with
Autor:
Charles Vinson, Asha Acharya, Dominic A. Scudiero, Julie Laudeman, Andrew G. Stephen, Paula M. Krosky, Thomas Silvers, Russel Reinhart, Michael Selby, Timothy M. Potter, Won Jun Oh, Jonathan R. Moll, Robert H. Shoemaker, Timothy Stevenson, Vikas Rishi
Publikováno v:
Analytical Biochemistry. 340:259-271
We have developed a high-throughput fluorescence anisotropy screen, using a 384-well format, to identify small molecules that disrupt the DNA binding of B-ZIP proteins. Binding of a B-ZIP dimer to fluorescently labeled DNA can be monitored by fluores
Autor:
Jonathan R. Moll, Charles Vinson, David Landsman, Jan S. Fassler, Maria Bonovich, Asha Acharya
Publikováno v:
Scopus-Elsevier
Basic region-leucine zipper (B-ZIP) transcription factors bind as dimers to sequence-specific DNA and regulate gene expression. The transcriptional potential of B-ZIP proteins is often regulated by posttranslational phosphorylation in response to cel
Publikováno v:
Nucleic Acids Research. 30:1240-1246
We have examined binding of the CREB B-ZIP protein domain to double-stranded DNA containing a consensus CRE sequence (5'-TGACGTCA-3'), the related PAR, C/EBP and AP-1 sequences and the unrelated SP1 sequence. DNA binding was assayed in the presence o
Publikováno v:
Biochemical Journal. 360:599-607
The activator protein 1 (AP-1) transcription factor is composed of heterodimers of the Fos/activating transcription factor (ATF) and Jun subfamilies of basic-region leucine-zipper (B-ZIP) proteins. In order to determine the identities of individual B
Publikováno v:
Journal of Biological Chemistry. 275:17814-17820
The in vivo pattern of induction of phosphoenolpyruvate carboxykinase (PEPCK) gene transcription by cAMP and its inhibition by insulin is reproduced in H4IIe cells and is mediated by a bipartite cAMP/insulin response unit (C/IRU) consisting of a cAMP
Autor:
Charles Vinson, Shizuko Tanaka, Dmitri Penkov, Maurizio Di Liberto, Jonathan R. Moll, Wen Zhang, Francesco Ramirez, Michelle Olive, Patricia Greenwel
Publikováno v:
Molecular and Cellular Biology. 20:912-918
Extracellular matrix (ECM) formation and remodeling are critical processes for proper morphogenesis, organogenesis, and tissue repair. The proinflammatory cytokine tumor necrosis factor alpha (TNF-a) inhibits ECM accumulation by stimulating the expre
Autor:
Andrew C. Nyborg, Alan S. Rudolph, Giora Z. Feuerstein, Daniel Havas, Jonathan R. Moll, Renee Wegrzyn, Birgit Hutter-Paier
Publikováno v:
Journal of Alzheimer's disease : JAD. 34(4)
Accumulation of amyloid-β (Aβ) cascade aggregates is considered a hallmark of Alzheimer's disease (AD). Current dogma holds that the appearance of Aβ oligomers and larger aggregates occur many years prior to plaque formation associated with the ad