Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Jonathan M. Kendall"'
Publikováno v:
Trends in Biotechnology. 16:216-224
Bioluminescence has revolutionized research into many cellular and molecular-biological processes, ranging from intracellular signalling to gene transcription. This article focuses on the chemistry and biotechnological exploitation of the two protein
Publikováno v:
Journal of Biological Chemistry. 273:29822-29829
The cytoplasmic calcium environments along membrane trafficking pathways leading to gap junction intercellular communication channels at the plasma membrane were studied. Connexins, the constitutive proteins of gap junctions, were fused at their carb
Publikováno v:
Cell Calcium. 23:79-86
We review and present currentevidence supporting independentregulation of nuclear Ca2+ ([Ca2+]n). The nucleus and nuclear envelope contain proteins to both regulate and respond to changes in [Ca2+]n]. However, this does not prove that [Ca2+]n is inde
Autor:
Anthony K. Campbell, Jonathan M. Kendall, Graciela B. Sala-Newby, Gavin William Grahame Wilkinson, Michael Norman Badminton
Publikováno v:
Cell Calcium. 19:133-142
Changes in the concentration of calcium ions ([Ca2+]) within cellular organelles play a central role in controlling cellular function. We have engineered the Ca2+ sensitive photoprotein aequorin to monitor selectively [Ca2+] within defined subcellula
Publikováno v:
Experimental Cell Research. 216:236-243
The Ca(2+)-activated photoprotein aequorin has been targeted to the nucleus of live cells by engineering nucleoplasmin, a nuclear structural protein from Xenopus laevis, onto the amino terminus. Successful targeting of the apoprotein was demonstrated
Autor:
Jonathan M. Kendall, Graciela B. Sala-Newby, Robert L. Dormer, Veena Ghalaut, Anthony K. Cambell
Publikováno v:
Biochemical and Biophysical Research Communications. 187:1091-1097
Two stage PCR has been used to introduce single amino acid substitutions into the EF hand structures of the Ca(2+)-activated photoprotein aequorin. Transcription of PCR products, followed by cell free translation of the mRNA, allowed characterisation
Autor:
Juan Diez, Christopher H. George, Jonathan M. Kendall, Patricia E. M. Martin, William Howard Evans, Shoeb Ahmad
Publikováno v:
Novartis Foundation Symposium 219-Gap Junction-Mediated Intercellular Signalling in Health and Disease
This chapter reports the mechanisms resulting in the assembly of gap junction intercellular communication channels. The connexin channel protein subunits are required to oligomerize into hexameric hemichannels (connexons) that may be homo- or heterom
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::eeb7c40fd8a98f55dc2d10dff1377b0e
https://doi.org/10.1002/9780470515587.ch4
https://doi.org/10.1002/9780470515587.ch4
Publikováno v:
Methods in enzymology. 414
To maximize the potential of high-content cellular analysis for investigating complex cellular signaling pathways and processes, we have generated a library of adenoviral encoded cellular sensors based on protein translocation and reporter gene activ
Publikováno v:
Biochemical and biophysical research communications. 268(3)
Apoaequorin was targeted to the cytosol, nucleus, and endoplasmic reticulum of HeLa cells in order to determine the effect of Ca(2+) release from the ER on protein degradation. In resting cells apoaequorin had a rapid half-life (ca. 20-30 min) in the
Autor:
Helen E. Jones, Anthony K. Campbell, Christopher H. George, William Howard Evans, Graciela B. Sala-Newby, Jonathan M. Kendall, A. R. Ribeiro, Michael Norman Badminton
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::daeacd6f260272c913c2fdd41581710b
https://doi.org/10.1016/s0076-6879(00)05508-7
https://doi.org/10.1016/s0076-6879(00)05508-7