Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Jonathan M. Craig"'
Autor:
Hinako Kawabe, Christopher A. Thomas, Shuichi Hoshika, Myong-Jung Kim, Myong-Sang Kim, Logan Miessner, Nicholas Kaplan, Jonathan M. Craig, Jens H. Gundlach, Andrew H. Laszlo, Steven A. Benner, Jorge A. Marchand
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-16 (2023)
Abstract The 4-letter DNA alphabet (A, T, G, C) as found in Nature is an elegant, yet non-exhaustive solution to the problem of storage, transfer, and evolution of biological information. Here, we report on strategies for both writing and reading DNA
Externí odkaz:
https://doaj.org/article/089902ffac324fbb99cc17565a7c89f5
Autor:
Jonathan M Craig, Maria Mills, Hwanhee C Kim, Jesse R Huang, Sarah J Abell, Jonathan W Mount, Jens H Gundlach, Keir C Neuman, Andrew H Laszlo
Publikováno v:
Nucleic Acids Research. 50:10601-10613
Helicases are essential for nearly all nucleic acid processes across the tree of life, yet detailed understanding of how they couple ATP hydrolysis to translocation and unwinding remains incomplete because their small (∼300 picometer), fast (∼1 m
Autor:
Christopher A. Thomas, Jonathan M. Craig, Shuichi Hoshika, Henry Brinkerhoff, Jesse R. Huang, Sarah J. Abell, Hwanhee C. Kim, Michaela C. Franzi, Jessica D. Carrasco, Hyo-Joong Kim, Drew C. Smith, Jens H. Gundlach, Steven A. Benner, Andrew H. Laszlo
Publikováno v:
Journal of the American Chemical Society.
Autor:
Alan Shaw, Jonathan M. Craig, Hossein Amiri, Jeonghoon Kim, Heather E. Upton, Sydney C. Pimentel, Jesse R. Huang, Susan Marqusee, Jens H. Gundlach, Kathleen Collins, Carlos J. Bustamante
Techniques that can directly sequence RNAs and determine secondary structures are essential to establish how an RNA molecule folds and how folding affects its function. We describe the development of a direct RNA nanopore sequencing technique using a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5aaa4a7f0575975f04797cedb8dcba36
https://doi.org/10.1101/2023.04.05.535757
https://doi.org/10.1101/2023.04.05.535757
Autor:
Sinduja K. Marx, Keith J. Mickolajczyk, Jonathan M. Craig, Christopher A. Thomas, Akira M. Pfeffer, Sarah J. Abell, Jessica D. Carrasco, Michaela C. Franzi, Jesse R. Huang, Hwanhee C. Kim, Henry D. Brinkerhoff, Tarun M. Kapoor, Jens H. Gundlach, Andrew H. Laszlo
Publikováno v:
bioRxiv : the preprint server for biology.
The genome of SARS-CoV-2 encodes for a helicase called nsp13 that is essential for viral replication and highly conserved across related viruses, making it an attractive antiviral target. Here we use nanopore tweezers, a high-resolution single-molecu
Autor:
Joseph H Chapman, Jonathan M Craig, Clara D Wang, Jens H Gundlach, Keir C Neuman, J Robert Hogg
Publikováno v:
Nucleic Acids Res
The conserved RNA helicase UPF1 coordinates nonsense-mediated mRNA decay (NMD) by engaging with mRNAs, RNA decay machinery and the terminating ribosome. UPF1 ATPase activity is implicated in mRNA target discrimination and completion of decay, but the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fb67d8af1a30ed6ebb0609d89f3e59a
https://doi.org/10.1101/2022.07.08.499162
https://doi.org/10.1101/2022.07.08.499162
Publikováno v:
Essays Biochem
Single-molecule picometer resolution nanopore tweezers (SPRNT) is a technique for monitoring the motion of individual enzymes along a nucleic acid template at unprecedented spatiotemporal resolution. We review the development of SPRNT and the applica
Autor:
Ian C Nova, Ian M Derrington, Jonathan M Craig, Matthew T Noakes, Benjamin I Tickman, Kenji Doering, Hugh Higinbotham, Andrew H Laszlo, Jens H Gundlach
Publikováno v:
PLoS ONE, Vol 12, Iss 7, p e0181599 (2017)
Nanopore DNA sequencing is a promising single-molecule analysis technology. This technique relies on a DNA motor enzyme to control movement of DNA precisely through a nanopore. Specific experimental buffer conditions are required based on the preferr
Externí odkaz:
https://doaj.org/article/560b97e04cbb4d4a8f44a96e86638980
Autor:
Andrew H. Laszlo, Jonathan M. Craig, Momčilo Gavrilov, Ramreddy Tippana, Ian C. Nova, Jesse R. Huang, Hwanhee C. Kim, Sarah J. Abell, Mallory deCampos-Stairiker, Jonathan W. Mount, Jasmine L. Bowman, Katherine S. Baker, Hugh Higinbotham, Dmitriy Bobrovnikov, Taekjip Ha, Jens H. Gundlach
We used single-molecule picometer-resolution nanopore tweezers (SPRNT) to resolve the millisecond single-nucleotide steps of superfamily 1 helicase PcrA as it translocates on, or unwinds, several kilobase-long DNA molecules. We recorded more than two
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17d0b1870c4e8e88f72ee1da32ff0d82
https://doi.org/10.1101/2022.02.10.479955
https://doi.org/10.1101/2022.02.10.479955
Autor:
Jesse R. Huang, Hwanhee C. Kim, Maria Mills, Jonathan M. Craig, Jonathan W. Mount, Andrew H. Laszlo, Keir C. Neuman, Sarah J. Abell, Jens H. Gundlach
Helicases are essential for nearly all nucleic acid processes across the tree of life. Using Nanopore Tweezers we observed the small, fast steps taken by single RecQ helicases as they step along and unwind DNA at ultrahigh spatiotemporal resolution.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bbd06c5933fd87348a0e8b3b21ba2132
https://doi.org/10.1101/2021.09.23.461529
https://doi.org/10.1101/2021.09.23.461529