Total wash elimination for solid phase peptide synthesis

Autor: Jonathan M. Collins, Sandeep K. Singh, Travis A. White, Drew J. Cesta, Colin L. Simpson, Levi J. Tubb, Christopher L. Houser

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-11 (2023)

Abstract We present a process for solid phase peptide synthesis (SPPS) that completely eliminates all solvent intensive washing steps during each amino acid addition cycle. A key breakthrough is the removal of a volatile Fmoc deprotection base throug

Externí odkaz: https://doaj.org/article/a55cb64e56434741a270699aa58ecd92

Cleaving protected peptides from 2-chlorotrityl chloride resin. Moving away from dichloromethane

Autor: Mahama Alhassan, Beatriz G. de la Torre, Othman Al Musaimi, Jonathan M. Collins, Fernando Albericio

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
instname
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)

In recent years, the work of various research groups has allowed the substitution of the hazardous solvents most widely used in solid-phase peptide synthesis, namely DMF, NMP, DCM, DEE, among others, by several much less hazardous solvents. Indeed, g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49ec5020351952cf82f89e08252ed755
https://doi.org/10.1039/d0gc00834f

New Developments in Microwave-Assisted Solid Phase Peptide Synthesis

Autor: Sandeep K, Singh, Jonathan M, Collins

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2103

The unique combination of microwave heating with optimized carbodiimide activation has proven to be an indispensable technique for high-throughput peptide production. Here, we describe new methods in microwave-assisted solid phase peptide synthesis a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3c73255cd24ddfec1b5fbe18d02d9cfe
https://pubmed.ncbi.nlm.nih.gov/31879920

Microwave-Assisted Green Solid-Phase Peptide Synthesis Using γ-Valerolactone (GVL) as Solvent

Autor: Jonathan M. Collins, Beatriz G. de la Torre, Yahya E. Jad, Fernando Albericio, Ashok Kumar

Publikováno v: ACS Sustainable Chemistry & Engineering. 6:8034-8039

The increasing demand for synthetic peptides in various research fields raises a challenge, namely, the development of greener methods for their preparation. Here we report on an ecofriendly solid-phase peptide synthesis (SPPS) methodology. Substitut

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b0d8059447367fbd2e7dc7b12356e977
https://doi.org/10.1021/acssuschemeng.8b01531

Investigating green ethers for the precipitation of peptides after global deprotection in solid-phase peptide synthesis

Autor: Fernando Albericio, Jonathan M. Collins, Beatriz G. de la Torre, Othman Al Musaimi, Yahya E. Jad, Alessandra Basso, Ashok Kumar

Diethyl ether and methyl tert-butyl ether are the solvents most widely used for peptide precipitation after global deprotection in the last step of solid-phase peptide synthesis. Here we report peptide precipitation with the greener solvent cyclopent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3517ec5275fda9be69d0c0e9e16d2fd4
http://hdl.handle.net/10044/1/88627