Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Jonathan K. Fleming"'
Autor:
Heather M. Callaway, Kathryn M. Hastie, Sharon L. Schendel, Haoyang Li, Xiaoying Yu, Jeremy Shek, Tierra Buck, Sean Hui, Dan Bedinger, Camille Troup, S. Moses Dennison, Kan Li, Michael D. Alpert, Charles C. Bailey, Sharon Benzeno, Jody L. Bonnevier, Jin-Qiu Chen, Charm Chen, Hyeseon Cho, Peter D. Crompton, Vincent Dussupt, Kevin C. Entzminger, Yassine Ezzyat, Jonathan K. Fleming, Nick Geukens, Amy E. Gilbert, Yongjun Guan, Xiaojian Han, Christopher J. Harvey, Julia M. Hatler, Bryan Howie, Chao Hu, Ailong Huang, Maya Imbrechts, Aishun Jin, Nik Kamachi, Gladys Keitany, Mark Klinger, Jay K. Kolls, Shelly J. Krebs, Tingting Li, Feiyan Luo, Toshiaki Maruyama, Michael A. Meehl, Letzibeth Mendez-Rivera, Andrea Musa, C.J. Okumura, Benjamin E.R. Rubin, Aaron K. Sato, Meiying Shen, Anirudh Singh, Shuyi Song, Joshua Tan, Jeffrey M. Trimarchi, Dhruvkumar P. Upadhyay, Yingming Wang, Lei Yu, Tom Z. Yuan, Erik Yusko, Bjoern Peters, Georgia Tomaras, Erica Ollmann Saphire
Publikováno v:
Cell Reports, Vol 42, Iss 1, Pp 112014- (2023)
Summary: The SARS-CoV-2 Omicron variant of concern (VoC) and its sublineages contain 31–36 mutations in spike and escape neutralization by most therapeutic antibodies. In a pseudovirus neutralization assay, 66 of the nearly 400 candidate therapeuti
Externí odkaz:
https://doaj.org/article/61e61f138daa45d1ba9a2edb473cf0bc
Autor:
Teruo Akuta, Toshiaki Maruyama, Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Kevin Entzminger, Jonathan K. Fleming, Ryo Sato, Takashi Shibata, Yasunori Kurosawa, C. J. Okumura, Tsutomu Arakawa
Publikováno v:
Antibodies, Vol 11, Iss 2, p 36 (2022)
In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the
Externí odkaz:
https://doaj.org/article/906228a9cdd34f64873d8605805429af
Autor:
Ashlee N. King, Jonathan K. Fleming, Stephanie S. Knapik, Barbara Visentin, Jonathan M. Wojciak, Tom Huxford
Publikováno v:
Journal of Lipid Research, Vol 58, Iss 7, Pp 1386-1398 (2017)
Cysteinyl leukotrienes (CysLTs) are a small family of biological signaling lipids produced by active leukocytes that contribute to diverse inflammatory disease states as a consequence of their engagement with dedicated G protein-coupled receptors. Im
Externí odkaz:
https://doaj.org/article/56ee7b94a4b7451683b881628ed990e5
Publikováno v:
Journal of Lipid Research, Vol 57, Iss 9, Pp 1737-1747 (2016)
Sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) are bioactive signaling lysophospholipids that activate specific G protein-coupled receptors on the cell surface triggering numerous biological events. In circulation, S1P and LPA associat
Externí odkaz:
https://doaj.org/article/950f5f4f9b4c469faee15971b0df0f6e
Autor:
Elinaz Farokhi, Jonathan K. Fleming, M. Frank Erasmus, Aaron D. Ward, Yunjin Wu, Maria G. Gutierrez, Jonathan M. Wojciak, Tom Huxford
Publikováno v:
Antibodies, Vol 9, Iss 2, p 10 (2020)
LT1009 is a humanized version of murine LT1002 IgG1 that employs two bridging Ca2+ ions to bind its antigen, the biologically active lipid sphingosine-1-phosphate (S1P). We crystallized and determined the X-ray crystal structure of the LT1009 Fab fra
Externí odkaz:
https://doaj.org/article/3fd041eebce64951824ba2755c2ef9d9
Autor:
Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Toshiaki Maruyama, Kevin Entzminger, Jonathan K Fleming, Takashi Shibata, Yasunori Kurosawa, CJ Okumura, Tsutomu Arakawa, Teruo Akuta
Publikováno v:
BioTechniques, Vol 72, Iss 5, Pp 207-218 (2022)
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid. This gel electrophoresis successfully provid
Externí odkaz:
https://doaj.org/article/617c78fb8b3e44e2b265eac57674e82c
Autor:
Kevin C Entzminger, Jonathan K Fleming, Paul D Entzminger, Lisa Yuko Espinosa, Alex Samadi, Yuko Hiramoto, Shigeru C J Okumura, Toshiaki Maruyama
Publikováno v:
Antibody Therapeutics.
SARS-CoV-2 Omicron variant XBB.1.5 has shown extraordinary immune escape even for fully vaccinated individuals. There are currently no approved antibodies that neutralize this variant, and continued emergence of new variants puts immunocompromised an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6ee6343f72db2fe69605d3bec718efde
https://doi.org/10.1093/abt/tbad006
https://doi.org/10.1093/abt/tbad006
Autor:
Kevin C. Entzminger, Jonathan K. Fleming, Paul D. Entzminger, Lisa Yuko Espinosa, Alex Samadi, Yuko Hiramoto, CJ Okumura, Toshiaki Maruyama
An antibody panel that broadly neutralizes currently circulating Omicron variants was obtained byin vitroaffinity maturation using phage display. Starting from a single parent clone, antibody engineering was performed in iterative stages in real time
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cbfb3a788a22b39396c9c80a216adcdb
https://doi.org/10.1101/2023.01.25.525589
https://doi.org/10.1101/2023.01.25.525589
Publikováno v:
Journal of Lipid Research, Vol 57, Iss 9, Pp 1737-1747 (2016)
Sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) are bioactive signaling lysophospholipids that activate specific G protein-coupled receptors on the cell surface triggering numerous biological events. In circulation, S1P and LPA associat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3400d04a650569874d79779f43ecaf5
https://doi.org/10.1194/jlr.d068866
https://doi.org/10.1194/jlr.d068866
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1697
By directly detecting the ligand-free binding sites in a sample, the kinetic exclusion assay (KinExA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::346bcd9c366286fa2d09defb0ed18b3a
https://pubmed.ncbi.nlm.nih.gov/28349502
https://pubmed.ncbi.nlm.nih.gov/28349502