Zobrazeno 1 - 10
of 50
pro vyhledávání: '"Jonathan D. Chesnut"'
Publikováno v:
BioTechniques, Vol 65, Iss 5, Pp 259-267 (2018)
GUIDE-seq was developed to detect CRISPR/Cas9 off-target. However, as originally reported, it was associated with a high level of nonspecific amplification. In an attempt to improve it, we developed target-enriched GUIDE-seq (TEG-seq). The sensitivit
Externí odkaz:
https://doaj.org/article/63c3bc2431844672bd48958fa867531c
Autor:
Jenna Bradley, Mike Firth, Jian-Ping Yang, James Pilling, Jonathan D. Chesnut, Jan Wildenhain, David R. Piper, Mathew Leveridge, Patrick J. O'Shea, Chetana M. Revankar, Beverley Isherwood
Publikováno v:
SLAS Discovery. 25:618-633
CRISPR/Cas9 is increasingly being used as a tool to prosecute functional genomic screens. However, it is not yet possible to apply the approach at scale across a full breadth of cell types and endpoints. In order to address this, we developed a novel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fa13617615321cd50a6cb64e3cb3a59
https://doi.org/10.1177/2472555220926160
https://doi.org/10.1177/2472555220926160
Autor:
Jonathan D. Chesnut, Tat-Ming Lo, Wen Shan Yew, Matthew Wook Chang, In Young Hwang, Koon Jiew Chua, Wee Chiew Kwok, Jason Potter, Tao Sun
Publikováno v:
ACS synthetic biology. 9(7)
Chinese hamster ovary (CHO) cells are the superior host cell culture models used for the bioproduction of therapeutic proteins. One of the prerequisites for bioproduction using CHO cell lines is the need to generate stable CHO cell lines with optimal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1e348b5ac7f7dee0601a9728eb92f14
https://pubmed.ncbi.nlm.nih.gov/32470293
https://pubmed.ncbi.nlm.nih.gov/32470293
Publikováno v:
BioTechniques. 65:259-267
GUIDE-seq was developed to detect CRISPR/Cas9 off-target. However, as originally reported, it was associated with a high level of nonspecific amplification. In an attempt to improve it, we developed target-enriched GUIDE-seq (TEG-seq). The sensitivit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f069c8e319af30fadb14143cd405e1de
https://doi.org/10.2144/btn-2018-0105
https://doi.org/10.2144/btn-2018-0105
Publikováno v:
Journal of Biotechnology. 241:136-146
While CRISPR-based gene knock out in mammalian cells has proven to be very efficient, precise insertion of genetic elements via the cellular homology directed repair (HDR) pathway remains a rate-limiting step to seamless genome editing. Under the con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aca0e596783c1b330473c875365a9576
https://doi.org/10.1016/j.jbiotec.2016.11.011
https://doi.org/10.1016/j.jbiotec.2016.11.011
Autor:
Charles Neal Stewart, Kellie P. Burris, Jonathan D. Chesnut, Jian-Ping Yang, Reginald J. Millwood, Mary R. Rudis, Matthew H. Cheplick, Mitra Mazarei, Wusheng Liu, Christine A. Ondzighi-Assoume, Garrett A. Montgomery
Publikováno v:
Plant cell reports. 39(2)
A novel and robust lipofection-mediated transfection approach for the use of DNA-free Cas9/gRNA RNP for gene editing has demonstrated efficacy in plant cells. Precise genome editing has been revolutionized by CRISPR/Cas9 systems. DNA-based delivery o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b08acf3588032b8d093d4327afad5452
https://pubmed.ncbi.nlm.nih.gov/31728703
https://pubmed.ncbi.nlm.nih.gov/31728703
Publikováno v:
Analytical Biochemistry. 476:1-4
With the advent of synthetic biology and cell engineering, the demand for large synthetic DNA fragments has been steadily increasing. Consequently, a number of multi-fragment cloning technologies optimized for the assembly of sizable DNA constructs h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc86b7758b5f4177defa88ea77e8d86d
https://doi.org/10.1016/j.ab.2015.01.021
https://doi.org/10.1016/j.ab.2015.01.021
Autor:
Amar Ghodasara, Christopher A. Voigt, Velia Siciliano, Kevin Clancy, Liliana Wroblewska, Axel Trefzer, Jonathan D. Chesnut, Brynne C. Stanton, Ron Weiss
Publikováno v:
ACS Synthetic Biology
Prokaryotic regulatory proteins respond to diverse signals and represent a rich resource for building synthetic sensors and circuits. The TetR family contains >105 members that use a simple mechanism to respond to stimuli and bind distinct DNA operat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4431b066f7549411628d0b3021983714
https://doi.org/10.1021/sb5002856
https://doi.org/10.1021/sb5002856
Autor:
Wusheng Liu, Reginald J. Millwood, Wenzhi Xu, Jonathan D. Chesnut, Charles Neal Stewart, Yanhui Peng, Mitra Mazarei, Mary R. Rudis, Jian-Ping Yang
Publikováno v:
Plant Biotechnology Journal. 12:436-446
Transcription activator-like effectors (TALEs), secreted by the pathogenic bacteria Xanthomonas, specifically activate expression of targeted genes in plants. Here, we designed synthetic TALEs that bind to the flanking regions of the TATA-box motif o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37efd52ec7a2f1cbc2833ffa3b93eac2
https://doi.org/10.1111/pbi.12150
https://doi.org/10.1111/pbi.12150
Autor:
Andrew Fontes, Chad C. MacArthur, Jeanne F. Loring, Haipeng Xue, Xianmin Zeng, Louise C. Laurent, Miroslav Dudas, Jonathan D. Chesnut, Dennis Van Hoof, Pauline T. Lieu, Ying Liu, Andrzej Swistowski, Uma Lakshmipathy, Mahendra S. Rao, Rina Seerke, Thomas Touboul, Michael S. German
Publikováno v:
Stem Cells and Development. 21:191-205
Lineage reporters of human embryonic stem cell (hESC) lines are useful for differentiation studies and drug screening. Previously, we created reporter lines driven by an elongation factor 1 alpha (EF1α) promoter at a chromosome 13q32.3 locus in the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12fcb55d0371707dceeaf319f7431a34
https://doi.org/10.1089/scd.2011.0163
https://doi.org/10.1089/scd.2011.0163