Isolation, crystallization and crystal structure determination of bovine kidney Na+,K+-ATPase

Autor: Poul Nissen, Daniel Mattle, Natalya U. Fedosova, Jonas Lindholt Gregersen, Linda Reinhard

Publikováno v: Lindholt, J, Mattle, D, Fedosova, N, Nissen, P & Schuldt, L 2016, ' Isolation, crystallization and crystal structure determination of bovine kidney Na +,K +-ATPase ', Acta Crystallographica Section F: Structural Biology Communications, vol. 72, no. Pt. 4, pp. 282-287 . https://doi.org/10.1107/S2053230X1600279X
Acta crystallographica / F Structural biology communications Section F 72(4), 282-287 (2016). doi:10.1107/S2053230X1600279X

Acta crystallographica / F Structural biology communications Section F 72(4), 282 - 287 (2016). doi:10.1107/S2053230X1600279X
Na$^+$,K$^+$-ATPase is responsible for the transport of Na$^+$ and K$^+$ across the plasma membrane in animal cells, th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4be099af6b115c1229161a9d1edac16
https://doi.org/10.1107/s2053230x1600279x

Reconstitution of Na(+),K(+)-ATPase in Nanodiscs

Autor: Jonas Lindholt Gregersen, Natalya U. Fedosova, Poul Nissen, Thomas Boesen

Publikováno v: Gregersen, J L, Fedosova, N, Nissen, P & Boesen, T 2016, ' Reconstitution of Na,K-ATPase in Nanodiscs ', Methods in Molecular Biology, vol. 1377, pp. 403-409 .
Aarhus University

Nanodiscs are disc-shaped self-assembled lipid bilayers encircled by membrane scaffolding proteins derived from Apolipoprotein A-1 (apo A-1). They constitute a versatile tool for studying membrane proteins since reconstitution into nanodiscs allows s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::2b42d0e8d5d033a43d94694da859cebc
https://pubmed.ncbi.nlm.nih.gov/26695051

Structures and characterization of digoxin- and bufalin-bound Na+,K+-ATPase compared with the ouabain-bound complex

Autor: Poul Nissen, Jonas Lindholt Gregersen, Laure Yatime, Mette Laursen, Natalya U. Fedosova

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2015, 112 (6), pp.1755-1760. ⟨10.1073/pnas.1422997112⟩
Laursen, M, Gregersen, J L, Yatime, L, Nissen, P & Fedosova, N 2015, ' Structures and characterization of digoxin-and bufalin-bound Na +,K +-ATPase compared with the ouabain-bound complex ', PNAS (Proceedings of the National Academy of Sciences of the United States of America), vol. 112, no. 6, pp. 1755-1760 . https://doi.org/10.1073/pnas.1422997112

Cardiotonic steroids (CTSs) are specific and potent inhibitors of the Na+,K+-ATPase, with highest affinity to the phosphoenzyme (E2P) forms. CTSs are comprised of a steroid core, which can be glycosylated, and a varying number of substituents, includ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bb7158659f1d35d923d7cb680f4160f
https://pubmed.ncbi.nlm.nih.gov/25624492

Indications of radiation damage in ferredoxin microcrystals using high-intensity X-FEL beams

Autor: Thomas R. M. Barends, Garth J. Williams, Wolfgang Kabsch, Jonas Lindholt Gregersen, Daniel Mattle, Poul Nissen, Roberto Alonso-Mori, Stefan P. Hau-Riege, Saša Bajt, H. Olof Jönsson, Carl Caleman, Jason E. Koglin, Sébastien Boutet, Nikolaj D. Drachmann, Sabine Botha, Stephan Kassemeyer, Oleg Sitsel, Ilme Schlichting, Michael Krumrey, Marc Messerschmidt, Henry N. Chapman, Kenneth R. Beyerlein, Lutz Foucar, Anton Barty, Nicusor Timneanu, Dimosthenis Sokaras, Elisabeth Hartmann, Karol Nass, Andrew Aquila, Maike Bublitz, R. Bruce Doak, Richard Bean, Robert L. Shoeman, Linda Reinhard

Publikováno v: Nass, K, Foucar, L, Barends, T R M, Hartmann, E, Botha, S, Shoeman, R L, Doak, R B, Alonso-Mori, R, Aquila, A, Bajt, S, Barty, A, Bean, R, Beyerlein, K R, Bublitz, M, Drachmann, N, Gregersen, J, Jönsson, H O, Kabsch, W, Kassemeyer, S, Koglin, J E, Krumrey, M, Mattle, D, Messerschmidt, M, Nissen, P, Reinhard (maiden name Schuldt), L, Sitsel, O, Sokaras, D, Williams, G J, Hau-Riege, S, Timneanu, N, Caleman, C, Chapman, H N, Boutet, S & Schlichting, I 2015, ' Indications of radiation damage in ferredoxin microcrystals using high-intensity X-FEL beams ', Journal of Synchrotron Radiation, vol. 22, no. 2, pp. 225-238 . https://doi.org/10.1107/S1600577515002349
Journal of Synchrotron Radiation
Journal of synchrotron radiation 22(2), 225-238 (2015). doi:10.1107/S1600577515002349

Proteins that contain metal cofactors are expected to be highly radiation sensitive since the degree of X-ray absorption correlates with the presence of high-atomic-number elements and X-ray energy. To explore the effects of local damage in serial fe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4dca1faabfc67f555f9bbbbe0b80f52b
https://pubmed.ncbi.nlm.nih.gov/25723924

Comparing crystal structures of Ca²⁺-ATPase in the presence of different lipids.

Autor: Drachmann, Nikolaj D.^1,2, Olesen, Claus^1,3, Møller, Jesper V.^1,3, Guo, Zheng⁴, Nissen, Poul^1,2, Bublitz, Maike^1,2

Publikováno v: FEBS Journal. Sep2014, Vol. 281 Issue 18, p4249-4262. 14p.