Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Jon P. Cody"'
Autor:
James C. Chamness, Jitesh Kumar, Anna J. Cruz, Elissa Rhuby, Mason J. Holum, Jon P. Cody, Redeat Tibebu, Maria Elena Gamo, Colby G. Starker, Feng Zhang, Daniel F. Voytas
Publikováno v:
The Plant Genome, Vol 16, Iss 2, Pp n/a-n/a (2023)
Abstract Plant biotechnology is rife with new advances in transformation and genome engineering techniques. A common requirement for delivery and coordinated expression in plant cells, however, places the design and assembly of transformation constru
Externí odkaz:
https://doaj.org/article/cc0b892b4e544240b8309a81db06adbe
Autor:
Jon P. Cody, Michael F. Maher, Ryan A. Nasti, Colby G. Starker, James C. Chamness, Daniel F. Voytas
Publikováno v:
Nat Protoc
There is an expanding need to modify plant genomes to create new plant germplasm that advances both basic and applied plant research. Most current methods for plant genome modification involve regenerating plants from genetically modified cells in ti
Publikováno v:
Plant Direct, Vol 4, Iss 3, Pp n/a-n/a (2020)
Abstract Site‐specific recombinase enzymes function in heterologous cellular environments to initiate strand‐switching reactions between unique DNA sequences termed recombinase binding sites. Depending on binding site position and orientation, re
Externí odkaz:
https://doaj.org/article/98250771f54b48d79e75bc3faadca90f
Autor:
Ryan Nasti, Jon P. Cody, Matt H. Zinselmeier, Nikil B. Badey, Adhvaith Sridhar, Ambika Sharma, Michael F. Maher, Benjamin K. Blackman, Daniel F. Voytas
Tissue culture methods which serve as the standard to regenerate modified plants are challenging and have limited the capacity to engineer new accessions. To improve upon these techniques, genome modifying reagents have been combined with development
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::585ed620e4d82dad5922c5cab4f2624c
https://doi.org/10.1101/2022.11.15.516675
https://doi.org/10.1101/2022.11.15.516675
Autor:
James C. Chamness, Jitesh Kumar, Anna J. Cruz, Elissa Rhuby, Mason J. Holum, Jon P. Cody, Redeat Tibebu, Maria Elena Gamo, Colby G. Starker, Feng Zhang, Daniel F. Voytas
Plant biotechnology is rife with new advances in transformation and genome engineering techniques. A common requirement for delivery and coordinated expression in plant cells, however, places the design and assembly of transformation constructs at a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::961ac89f394d60cc502a4d7b5fc1996b
https://doi.org/10.1101/2022.10.15.511792
https://doi.org/10.1101/2022.10.15.511792
Autor:
Jon P. Cody, Nathan C. Swyers, Morgan E. McCaw, Nathaniel D. Graham, Changzeng Zhao, James A. Birchler
Publikováno v:
Agronomy, Vol 5, Iss 3, Pp 309-321 (2015)
Minichromosome technology has the potential to offer a number of possibilities for expanding current biofortification strategies. While conventional genome manipulations rely on random integration of one or a few genes, engineered minichromosomes wou
Externí odkaz:
https://doaj.org/article/bf928fdc086a4d8eb2685a0e73e6b8bd
Publikováno v:
Current protocols in plant biologyLiterature cited. 2(4)
Binary Bacterial Artificial Chromosomes (BiBAC) are large insert cloning vectors that contain the necessary features required for Agrobacterium-mediated transformation. However, the large size of BiBACs and low-copy number in Escherichia coli (DH10B)
Autor:
Nathan C. Swyers, Morgan E. McCaw, James A. Birchler, Jon P. Cody, Changzeng Zhao, Nathaniel D. Graham
Publikováno v:
Current Protocols in Plant Biology
Fluorescence In Situ Hybridization (FISH) is the annealing of fluorescent DNA probes to their complementary sequences on prepared chromosomes and subsequent visualization with a fluorescent microscope. In maize, FISH is useful for distinguishing each
Autor:
Nathan C. Swyers, Jon P. Cody, James A. Birchler, Morgan E. McCaw, Nathaniel D. Graham, Changzeng Zhao
Publikováno v:
Current Protocols in Plant Biology
High-quality preparations of chromosomes are useful for many purposes. To prepare metaphase chromosome spreads in maize, root tips are harvested and treated with nitrous oxide to stop cell division at metaphase before being fixed in acetic acid. This
Autor:
Jon P. Cody, Robert T. Gaeta, Morgan E. McCaw, Nathan C. Swyers, Changzeng Zhao, James A. Birchler, Nathaniel D. Graham
Publikováno v:
Current Protocols in Plant Biology
Minichromosomes have been generated in maize using telomere-mediated truncation. Telomere DNA, because of its repetitive nature, can be difficult to manipulate. The protocols in this unit describe two methods for generating the telomere DNA required