Výsledky vyhledávání - "Jolene A. Bradford"

Akademický článek

Fast-screening flow cytometry method for detecting nanoplastics in human peripheral blood

Autor: Roser Salvia, Laura G. Rico, Jolene A. Bradford, Michael D. Ward, Michael W. Olszowy, Cristina Martínez, Álvaro Domingo Madrid-Aris, Joan R. Grífols, Águeda Ancochea, Laia Gomez-Muñoz, Marta Vives-Pi, Eva Martínez-Cáceres, Marco A. Fernández, Marc Sorigue, Jordi Petriz

Publikováno v: MethodsX, Vol 10, Iss , Pp 102057- (2023)

Plastic pollution is a global problem. Animals and humans can ingest and inhale plastic particles, with uncertain health consequences. Nanoplastics (NPs) are particles ranging from 1 nm to 1000 nm that result from the erosion or breakage of larger pl

Externí odkaz: https://doaj.org/article/95b23a72ff19416b95960e7397f051fa

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Akademický článek

Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation

Autor: Laura G. Rico, Roser Salvia, Michael D. Ward, Jolene A. Bradford, Jordi Petriz

Publikováno v: STAR Protocols, Vol 2, Iss 4, Pp 100883- (2021)

Summary: This protocol provides instructions to improve flow cytometry analysis of marrow/peripheral blood cells by avoiding erythrolytic solutions, density gradients, and washing steps. We describe two basic approaches for identifying cell surface a

Externí odkaz: https://doaj.org/article/2f454dda604e4ae5b90cdc008b5d3de4

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Akademický článek

Unmasking the expression of PD-L1 in Myeloid Derived Suppressor Cells: A case study in lung cancer to discover new drugs with specific on-target efficacy

Autor: Laura G. Rico, Andrés Aguilar Hernández, Michael D. Ward, Jolene A. Bradford, Jordi Juncà, Rafael Rosell, Jordi Petriz

Publikováno v: Translational Oncology, Vol 14, Iss 1, Pp 100969- (2021)

Externí odkaz: https://doaj.org/article/aba8c71271b5444db4c5142eebdb4cce

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Erythrocyte lysing solutions have a detrimental effect in flow cytometric dendritic cell detection

Autor: Laura G. Rico, Roser Salvia, Jordi Juncà, Mike D. Ward, Jolene A. Bradford, Marc Sorigue, Jordi Petriz

Publikováno v: Cytometry Part A. 103:383-391

Flow cytometry (FCM) enumeration of peripheral blood dendritic cells (PBDCs) is a minimally invasive procedure extremely useful for immunological studies. Numbers of PBDCs vary depending on age, lifestyle, or in pathologies like cancer, leukemia or i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c28f60ae6dc25bdcc32b6b0f20c4689
https://doi.org/10.1002/cyto.a.24696

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Effects of rapid gravity load changes on immunophenotyping and leukocyte function of human peripheral blood after parabolic flight

Autor: Abril Gorgori-González, Antoni Perez-Poch, Daniel V. González, Roser Salvia, Laura G. Rico, Michael D. Ward, Jolene A. Bradford, Jordi Petriz, Ginés Viscor

Publikováno v: Acta Astronautica.

One of the biological systems that suffers a physiological de-conditioning in space is the immune system. It is in charge of defending the body against pathogens and other aggressions. The aim of this work is to assess if there are any relevant chang

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d0f40ab01f7dec11eab15eae43e60b2
https://doi.org/10.1016/j.actaastro.2023.02.012

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Impact of red blood cell lysing on rare event analysis

Autor: Laura G. Rico, Fernando Raimúndez de la Calle, Roser Salvia, Mike D. Ward, Jolene A. Bradford, Jordi Juncà, Marc Sorigue, Jordi Petriz

Publikováno v: Cytometry Part A
r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol
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The challenges associated with analyzing rare cells are dependent on a series of factors, which usually require large numbers of cells per sample for successful resolution. Among these is determining the minimum number of total events needed to be ac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3269b676ac4c6ec723a17242e7b35cf
https://fundanet.igtp.cat/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=6780

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Accurate identification of cell doublet profiles: Comparison of light scattering with fluorescence measurement techniques

Autor: Laura G. Rico, Jorge Bardina, Àngel Bistué‐Rovira, Roser Salvia, Mike D. Ward, Jolene A. Bradford, Jordi Petriz

Publikováno v: Cytometry Part A
r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol
instname

Doublet discrimination is usually based on pulse analysis of light scatter parameters. A combination of two pulse parameters (Area, A; Height, H; or Width, W) can be used to discriminate a pulse originated in a single cell from a pulse originated fro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50941ccba99d988cbfa79110038e31fc
https://fundanet.igtp.cat/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=6785

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Myeloid Derived Suppressor Cells in Lung Adenocarcinoma

Autor: Laura G.Rico, Andres Aguilar Hernandez, Michael D. Ward, Jolene A. Bradford, Jordi Junca, Rafael Rosell, Jordi Petriz

Immunohistochemicaland flow cytometric assessment of PD-L1 expression on tumor cells and immunecells might suppose a great benefit to identify patients undergoing immunotherapies. However, the use of different techniques and antibodies cancomplicate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c2824de2a88a19667892835ad0741bc4
https://doi.org/10.31219/osf.io/qtj35

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PD-L1 expression at the single cell level in Myeloid Derived Suppressor Cells using a stimulatory functional assay to evaluate immunotherapy strategies in multiple myeloma

Autor: Jolene A Bradford, Laura G. Rico, Jordi Junca, Jorge Bardina, Angel Bistue-Rovira, Michael D. Ward, Jordi Petriz

Publikováno v: The Journal of Immunology. 204:145.25-145.25

Background Multiple myeloma (MM) is characterized by malignant plasma cells in the bone marrow with an associated immunosuppressive microenvironment. PD-L1 suppresses immune response and is overexpressed in MM plasma cells and MDSCs. PD-L1 plays an i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bde84a333ffdab022caa118ce976dfa7
https://doi.org/10.4049/jimmunol.204.supp.145.25

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Evaluation of Cytometer Sensitivity and Stability using Automated Analysis

Autor: Jolene A Bradford, Justin Meskas, Patricia Sardina

Publikováno v: The Journal of Immunology. 202:131.41-131.41

Flow cytometers are standard tools of the immunologist, providing simultaneous measurement of many targets. The performance of a flow cytometer may vary between instruments, or over time within the same instrument. Evaluation of cytometer performance

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::584a41d702da5fc434739275544a7663
https://doi.org/10.4049/jimmunol.202.supp.131.41

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