Tandem LIM domain-containing proteins, LIMK1 and LMO1, directly bind to force-bearing keratin intermediate filaments

Autor: Dah Som Kim, Joleen S. Cheah, Tzu Wei Lai, Karen X. Zhao, Skylar R. Foust, Yuh-Ru Julie Lee, Su Hao Lo, Volkmar Heinrich, Soichiro Yamada

Publikováno v: Cell Reports, Vol 43, Iss 7, Pp 114480- (2024)

Summary: The cytoskeleton of the cell is constantly exposed to physical forces that regulate cellular functions. Selected members of the LIM (Lin-11, Isl-1, and Mec-3) domain-containing protein family accumulate along force-bearing actin fibers, with

Externí odkaz: https://doaj.org/article/69caa2fdf41540a4b0a2a01fdd161698

Dynamic mapping of proteome trafficking within and between living cells by TransitID

Autor: Wei Qin Xu, Joleen S. Cheah, Charles Xu, James Messing, Brian D. Freibaum, Steven Boeynaems, J. Paul Taylor, Namrata D. Udeshi, Steven A. Carr, Alice Y. Ting

Publikováno v: bioRxiv

SummaryThe ability to map trafficking for thousands of endogenous proteins at once in living cells would reveal biology currently invisible to both microscopy and mass spectrometry. Here we report TransitID, a method for unbiased mapping of endogenou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::699c0fbaeae381cb59089e492a52fc49
https://europepmc.org/articles/PMC9934598/

Inside-out regulation of E-cadherin conformation and adhesion

Autor: Joleen S. Cheah, Chi-Fu Yen, Willem-Jan Pannekoek, Martijn Gloerich, Andrew Vae Priest, Ramesh Koirala, Sanjeevi Sivasankar, Soichiro Yamada

Publikováno v: Proc Natl Acad Sci U S A

Cadherin cell-cell adhesion proteins play key roles in tissue morphogenesis and wound healing. Cadherin ectodomains bind in two conformations, X-dimers and strand-swap dimers, with different adhesive properties. However, the mechanisms by which cells

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::052df82372d8123fc860dc403b6bc93c
https://pubmed.ncbi.nlm.nih.gov/34301871

Force-induced recruitment of cten along keratin network in epithelial cells

Autor: Kyle A. Jacobs, Joleen S Cheah, Su Hao Lo, Soichiro Yamada, Volkmar Heinrich

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, vol 116, iss 40

The cytoskeleton provides structural integrity to cells and serves as a key component in mechanotransduction. Tensins are thought to provide a force-bearing linkage between integrins and the actin cytoskeleton; yet, direct evidence of tensin’s role

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6028b4d825b62f3bb2759e45c3e7648
https://pubmed.ncbi.nlm.nih.gov/31527270