Tandem LIM domain-containing proteins, LIMK1 and LMO1, directly bind to force-bearing keratin intermediate filaments

Autor: Dah Som Kim, Joleen S. Cheah, Tzu Wei Lai, Karen X. Zhao, Skylar R. Foust, Yuh-Ru Julie Lee, Su Hao Lo, Volkmar Heinrich, Soichiro Yamada

Publikováno v: Cell Reports, Vol 43, Iss 7, Pp 114480- (2024)

Summary: The cytoskeleton of the cell is constantly exposed to physical forces that regulate cellular functions. Selected members of the LIM (Lin-11, Isl-1, and Mec-3) domain-containing protein family accumulate along force-bearing actin fibers, with

Externí odkaz: https://doaj.org/article/69caa2fdf41540a4b0a2a01fdd161698

Dynamic mapping of proteome trafficking within and between living cells by TransitID

Autor: Wei Qin Xu, Joleen S. Cheah, Charles Xu, James Messing, Brian D. Freibaum, Steven Boeynaems, J. Paul Taylor, Namrata D. Udeshi, Steven A. Carr, Alice Y. Ting

Publikováno v: bioRxiv

SummaryThe ability to map trafficking for thousands of endogenous proteins at once in living cells would reveal biology currently invisible to both microscopy and mass spectrometry. Here we report TransitID, a method for unbiased mapping of endogenou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::699c0fbaeae381cb59089e492a52fc49
https://europepmc.org/articles/PMC9934598/

Inside-out regulation of E-cadherin conformation and adhesion

Autor: Joleen S. Cheah, Chi-Fu Yen, Willem-Jan Pannekoek, Martijn Gloerich, Andrew Vae Priest, Ramesh Koirala, Sanjeevi Sivasankar, Soichiro Yamada

Publikováno v: Proc Natl Acad Sci U S A

Cadherin cell-cell adhesion proteins play key roles in tissue morphogenesis and wound healing. Cadherin ectodomains bind in two conformations, X-dimers and strand-swap dimers, with different adhesive properties. However, the mechanisms by which cells

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::052df82372d8123fc860dc403b6bc93c
https://pubmed.ncbi.nlm.nih.gov/34301871

Aquaporin-5 regulation of cell-cell adhesion proteins: an elusive 'tail' story

Autor: Joleen S Cheah, Johan Palmfeldt, Lene N. Nejsum, Soichiro Yamada, Frédéric H. Login

Publikováno v: Login, F H, Palmfeldt, J, Cheah, J, Yamada, S & Nejsum, L N 2021, ' Aquaporin-5 regulation of cell-cell adhesion proteins: an elusive "tail" story ', American Journal of Physiology: Cell Physiology, vol. 320, no. 3, pp. C282-C292 . https://doi.org/10.1152/ajpcell.00496.2020

Aquaporins (AQPs) are water channels that facilitate transport of water across cellular membranes. AQPs are overexpressed in several cancers. Especially in breast cancer, AQP5 overexpression correlates with spread to lymph nodes and poor prognosis. P

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdea134b16fb756678d3c7f1ddbcb2d7
https://pure.au.dk/portal/da/publications/aquaporin5-regulation-of-cellcell-adhesion-proteins-an-elusive-tail-story(e5f95ad6-fb9c-4c05-9944-ff847bbefda2).html

A simple elution strategy for biotinylated proteins bound to streptavidin conjugated beads using excess biotin and heat

Autor: Soichiro Yamada, Joleen S Cheah

Publikováno v: Biochemical and biophysical research communications, vol 493, iss 4

Protein-protein interactions are the molecular basis of cell signaling. Recently, proximity based biotin identification (BioID) has emerged as an alternative approach to traditional co-immunoprecipitation. In this protocol, a mutant biotin ligase pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5aec67c2a1872922d89f46906e139407
https://escholarship.org/uc/item/3bj17647