Reduced mGluR5 Activity Modulates Mitochondrial Function

Autor: Miguel A. Gonzalez-Lozano, Joke Wortel, Rolinka J. van der Loo, Jan R. T. van Weering, August B. Smit, Ka Wan Li

Publikováno v: Cells, Vol 10, Iss 6, p 1375 (2021)

The metabotropic glutamate receptor 5 (mGluR5) is an essential modulator of synaptic plasticity, learning and memory; whereas in pathological conditions, it is an acknowledged therapeutic target that has been implicated in multiple brain disorders. D

Externí odkaz: https://doaj.org/article/bc1d7a4f644842e2bd90c7bfd89c78bd

Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines

Autor: Ariane Pereira, Jeff A. Stogsdill, Gesine Saher, Peter Scheiffele, Lisa Traunmüller, Klaus-Armin Nave, Jiexin Wang, Chris J. McBain, Naosuke Hoshina, Huda Y. Zoghbi, Yu Itoh-Maruoka, Cagla Eroglu, Wei-Hsiang Huang, Fritz Benseler, Thomas Philips, Constance L. Cepko, Mateusz C. Ambrozkiewicz, Jeremy N. Kay, Hiroshi Kawabe, Vania F. Prado, Kevin T. Beier, Wenjia You, Nils Brose, Harold A. Burgess, Wei Lu, Junko Motohashi, Pascal S. Kaeser, Liqun Luo, Marco A. M. Prado, Elisabetta Furlanis, Emily Ling-Lin Pai, Kenji Sakimura, Kenji Mandai, John L.R. Rubenstein, Andrea M. Gomez, Emilie Dumontier, Michisuke Yuzaki, Hisashi Umemori, Yoshimi Takai, Jean-François Cloutier, Susanne Falkner, Laura A. Lavery, Sandra Goebbels, Matthijs Verhage, Ann Marie Craig, Cui Chen, Kenneth A. Pelkey, Wei Li, Nashat Abumaria, Joshua R. Sanes, Lin Luo, Jeffrey D. Rothstein, Joke Wortel, Jennifer L. Sinclair, Mary Anne Hutchison, Tomohiko Maruo

Publikováno v: Neuron, vol 106, iss 1
Luo, L, Ambrozkiewicz, M C, Benseler, F, Chen, C, Dumontier, E, Falkner, S, Furlanis, E, Gomez, A M, Hoshina, N, Huang, W H, Hutchison, M A, Itoh-Maruoka, Y, Lavery, L A, Li, W, Maruo, T, Motohashi, J, Pai, E L L, Pelkey, K A, Pereira, A, Philips, T, Sinclair, J L, Stogsdill, J A, Traunmüller, L, Wang, J, Wortel, J, You, W, Abumaria, N, Beier, K T, Brose, N, Burgess, H A, Cepko, C L, Cloutier, J F, Eroglu, C, Goebbels, S, Kaeser, P S, Kay, J N, Lu, W, Luo, L, Mandai, K, McBain, C J, Nave, K A, Prado, M A M, Prado, V F, Rothstein, J, Rubenstein, J L R, Saher, G, Sakimura, K, Sanes, J R, Scheiffele, P, Takai, Y, Umemori, H, Verhage, M, Yuzaki, M, Zoghbi, H Y, Kawabe, H & Craig, A M 2020, ' Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines : Prevalence of Germline Recombination and Influencing Factors ', Neuron, vol. 106, no. 1, pp. 37-65.e5 . https://doi.org/10.1016/j.neuron.2020.01.008
Neuron, 106(1), 37-65.e5. Cell Press
Neuron

The Cre-loxP system is invaluable for spatial and temporal control of gene knockout, knockin, and reporter expression in the mouse nervous system. However, we report varying probabilities of unexpected germline recombination in distinct Cre driver li

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18a0d62fde17242aab3eee01b2747766
https://doi.org/10.1016/j.neuron.2020.01.008

Strain Differences in Presynaptic Function

Autor: Priyanka Rao-Ruiz, Sabine Spijker, A. Mariette Lenselink, Ka Wan Li, Yvonne Gouwenberg, Maarten Loos, Huibert D. Mansvelder, Pim van Nierop, August B. Smit, Roel C. van der Schors, Joke Wortel, Diana C. Rotaru

Publikováno v: Journal of Biological Chemistry. 290:15635-15645

The inbred strains C57BL/6J and DBA/2J (DBA) display striking differences in a number of behavioral tasks depending on hippocampal function, such as contextual memory. Historically, this has been explained through differences in postsynaptic protein

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b1d91b783bf02eded13b66f9860359ba
https://doi.org/10.1074/jbc.m114.628776

Proteomics, ultrastructure, and physiology of hippocampal synapses in a fragile X syndrome mouse model reveal presynaptic phenotype

Autor: Maarten Loos, Rhiannon M. Meredith, Ka Wan Li, Heidi de Wit, Jianru Stahl-Zeng, Diana C. Rotaru, Huibert D. Mansvelder, Oleg I. Klychnikov, Carl D. Holmgren, Patricia Klemmer, August B. Smit, Roel C. van der Schors, Joke Wortel, Sabine Spijker

Publikováno v: Journal of Biological Chemistry, 286(29), 25495-25504. American Society for Biochemistry and Molecular Biology Inc.
Klemmer, P, Meredith, R M, Holmgren, C D, Klychnikov, O I, Stahl-Zeng, J, Loos, M, van der Schors, R C, Wortel, J, de Wit, H, Spijker, S, Rotaru, D C, Mansvelder, H D, Smit, A B & Li, K W 2011, ' Proteomics, ultrastructure, and physiology of hippocampal synapses in a fragile X syndrome mouse model reveal presynaptic phenotype ', Journal of Biological Chemistry, vol. 286, no. 29, pp. 25495-25504 . https://doi.org/10.1074/jbc.M110.210260

Fragile X syndrome (FXS), the most common form of hereditary mental retardation, is caused by a loss-of-function mutation of the Fmr1 gene, which encodes fragile X mental retardation protein (FMRP). FMRP affects dendritic protein synthesis, thereby c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad4403e0b7d6d9daae8c796e4766e7db
https://hdl.handle.net/1871/39860

Automated analysis of secretory vesicle distribution at the ultrastructural level

Autor: Heidi de Wit, Matthijs Verhage, Joke Wortel, L. Niels Cornelisse, Jan R.T. van Weering, Wouter J. H. Veldkamp, Rik Wijntjes

Publikováno v: van Weering, J R T, Wijntjes, R, de Wit, H, Wortel, J, Cornelisse, L N, Veldkamp, W J & Verhage, M 2008, ' Automated analysis of secretory vesicle distribution at the ultrastructural level ', Journal of Neuroscience Methods, vol. 173, pp. 83-90 . https://doi.org/10.1016/j.jneumeth.2008.05.022
Journal of Neuroscience Methods, 173, 83-90. Elsevier

Neuroendocrine cells like chromaffin cells and PC-12 cells are established models for transport, docking and secretion of secretory vesicles. In micrographs, these vesicles are recognized by their electron dense core. The analysis of secretory vesicl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::934ed8207d79972e60adcf97745511ff
https://doi.org/10.1016/j.jneumeth.2008.05.022