Zobrazeno 1 - 10
of 10
pro vyhledávání: '"John-Paul Denson"'
Autor:
Matthew Smith, José Sánchez Hernández, Simon Messing, Nitya Ramakrishnan, Brianna Higgins, Jennifer Mehalko, Shelley Perkins, Vanessa E. Wall, Carissa Grose, Peter H. Frank, Julia Cregger, Phuong Vi Le, Adam Johnson, Mukul Sherekar, Morgan Pagonis, Matt Drew, Min Hong, Stephanie R. T. Widmeyer, John-Paul Denson, Kelly Snead, Ivy Poon, Timothy Waybright, Allison Champagne, Dominic Esposito, Jane Jones, Troy Taylor, William Gillette
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-17 (2024)
Abstract The diversity of chemical and structural attributes of proteins makes it inherently difficult to produce a wide range of proteins in a single recombinant protein production system. The nature of the target proteins themselves, along with cos
Externí odkaz:
https://doaj.org/article/d73bb5aac7c74a0792d3cbf25a30ef55
Autor:
Timothy H. Tran, Albert H. Chan, Lucy C. Young, Lakshman Bindu, Chris Neale, Simon Messing, Srisathiyanarayanan Dharmaiah, Troy Taylor, John-Paul Denson, Dominic Esposito, Dwight V. Nissley, Andrew G. Stephen, Frank McCormick, Dhirendra K. Simanshu
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
The molecular details of the RAS-RAF interaction are still not fully understood. Here, the authors present crystal structures of wild-type and mutant KRAS in complex with the RAS-binding and membrane-interacting cysteine-rich domains of RAF1, and pro
Externí odkaz:
https://doaj.org/article/8075c5993bfb4f61bc8bb5d453321337
Autor:
Carleen Klumpp-Thomas, Heather Kalish, Matthew Drew, Sally Hunsberger, Kelly Snead, Michael P. Fay, Jennifer Mehalko, Anandakumar Shunmugavel, Vanessa Wall, Peter Frank, John-Paul Denson, Min Hong, Gulcin Gulten, Simon Messing, Jennifer Hicks, Sam Michael, William Gillette, Matthew D. Hall, Matthew J. Memoli, Dominic Esposito, Kaitlyn Sadtler
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Understanding the infection parameters and host responses against SARS-CoV-2 require data from large cohorts using standardized methods. Here, the authors optimize a serum ELISA protocol that has minimal cross-reactivity and flexible sample collectio
Externí odkaz:
https://doaj.org/article/7420895e7e2b40c9b1ef65c8d78c8fa0
Autor:
Kelly Snead, Vanessa Wall, Carleen Klumpp-Thomas, John-Paul Denson, Heather Kalish, Dominic Esposito, Matthew Drew, Anandakumar Shunmugavel, Jennifer Mehalko, Michael P. Fay, Jennifer L. Hicks, Sam Michael, Matthew D. Hall, Gulcin Gulten, William K. Gillette, Matthew J. Memoli, Sally Hunsberger, Kaitlyn Sadtler, Peter Frank, Min Hong, Simon Messing
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Nature Communications
medRxiv
Nature Communications
medRxiv
The extent of SARS-CoV-2 infection throughout the United States population is currently unknown. High quality serology is a key tool to understanding the spread of infection, immunity against the virus, and correlates of protection. Limited validatio
Autor:
Anandakumar Shunmugavel, Alison Han, Heather Kalish, William K. Gillette, Matthew D. Hall, Matthew Drew, Sam Michael, Kelly Snead, Jennifer L. Hicks, Barry I. Graubard, Susan Reed, Kyle Pauly, Carleen Klumpp-Thomas, Luz Angela Rosas, Holly Ann Baus, Jennifer A. Croker, Vanessa Wall, Olivia Belliveau, Michelle M. Kolberg, Jing Wang, Michael P. Fay, Kenneth M. Adusei, Rani Athota, Yan Li, Luca T. Giurgea, Lindsay Czajkowski, Nalyn Siripong, Jennifer Mehalko, Reid Simon, Tran B. Ngo, Eric W. Ford, Rachel Bean, Maria Karkanitsa, Steven E. Reis, Kaitlyn Sadtler, Andrew Kelly, Brittany Heffelfinger, Jameson Travers, Shannon Valenti, Adriana Cervantes-Medina, Peter Frank, Jacquelyn Spathies, Simon Messing, Ravi Lokwani, Sally Hunsberger, Cheryl Chairez, Monica Gouzoulis, Rocco Caldararo, John-Paul Denson, Dominic Esposito, Robert P. Kimberly, Matthew J. Memoli, Saifullah Shafiq
Publikováno v:
medRxiv
Asymptomatic SARS-CoV-2 infection and delayed implementation of diagnostics have led to poorly defined viral prevalence rates. To address this, we analyzed seropositivity in US adults who have not previously been diagnosed with COVID-19. Individuals
Autor:
Matthew Drew, Vanessa Wall, Kaitlyn Sadtler, Troy Taylor, Dominic Esposito, William K. Gillette, John-Paul Denson, Kelly Snead, Simon Messing, Jennifer Mehalko
Publikováno v:
Protein Expression and Purification
bioRxiv
article-version (status) pre
article-version (number) 1
bioRxiv
article-version (status) pre
article-version (number) 1
The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is a commonly used antigen for serology assays critical to determining the extent of SARS-CoV-2 exposure in the population. Different versions of the RBD protein have been developed an
Autor:
Shannon Valenti, Luz Angela Rosas, Nalyn Siripong, Lindsay Czajkowski, Olivia Belliveau, Kyle Pauly, Carleen Klumpp-Thomas, Kenneth M. Adusei, Saifullah Shafiq, Jing Wang, Jameson Travers, Ravi Lokwani, Matthew Drew, Maria Karkanitsa, Reid Simon, Vanessa Wall, Yan Li, Steven E. Reis, Luca T. Giurgea, Michelle M. Kolberg, Brittany Heffelfinger, Matthew J. Memoli, Rachel Bean, Dominic Esposito, Matthew D. Hall, Monica Gouzoulis, Jennifer L. Hicks, Andrew Kelly, Barry I. Graubard, Susan Reed, Robert P. Kimberly, Sam Michael, Jacquelyn Spathies, Kaitlyn Sadtler, Rani Athota, Kelly Snead, Simon Messing, Adriana Cervantes-Medina, Jennifer A. Croker, Holly Ann Baus, Peter Frank, Michael P. Fay, Sally Hunsberger, Cheryl Chairez, Jennifer Mehalko, Tran B. Ngo, Eric W. Ford, William K. Gillette, Anandakumar Shunmugavel, Rocco Caldararo, Alison Han, John-Paul Denson, Heather Kalish
Publikováno v:
Science Translational Medicine
16.8 million SARS-CoV-2 infections in the US went undiagnosed in the first 6 months of the pandemic compared to 3.5 million diagnosed infections.
Elucidating seroprevalence in COVID-19 Symptoms of SARS-CoV-2 infection range from completely asymp
Elucidating seroprevalence in COVID-19 Symptoms of SARS-CoV-2 infection range from completely asymp
K-Ras4B Remains Monomeric on Membranes over a Wide Range of Surface Densities and Lipid Compositions
Autor:
Young Kwang Lee, Jay T. Groves, John-Paul Denson, Steven Alvarez, Jean K. Chung, Andrew G. Stephen, William K. Gillette
Ras is a membrane-anchored signaling protein that serves as a hub for many signaling pathways and also plays a prominent role in cancer. The intrinsic behavior of Ras on the membrane has captivated the biophysics community in recent years, especially
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::816edaa822fccb4aa7c080ef9ba86578
https://europepmc.org/articles/PMC5984903/
https://europepmc.org/articles/PMC5984903/
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1586
The major goal of any protein expression experiment is to combine the maximum production per cell of soluble protein with the highest possible cell density to most efficiently obtain high yields of protein. A large number of parameters can be optimiz
Autor:
Saulius Vainauskas, Kathryn C. Zoon, Dominic Esposito, Christopher H. Taron, Troy Taylor, Hana Schmeisser, Jeremiah D. Read, John-Paul Denson, Mehul B. Ganatra, Julia M. Hong, James L. Hartley
Publikováno v:
FEMS Yeast Research. 11:168-178
Secretion of recombinant proteins is a common strategy for heterologous protein expression using the yeast Kluyveromyces lactis. However, a common problem is degradation of a target recombinant protein by secretory pathway aspartyl proteases. In this