Zobrazeno 1 - 10
of 99
pro vyhledávání: '"John Z, Metcalfe"'
Autor:
Jason D. Limberis, John Z. Metcalfe
Publikováno v:
HardwareX, Vol 19, Iss , Pp e00576- (2024)
turboLysis is a novel mechanical cell lysis device that utilizes small beads to efficiently lyse tough cells like Mycobacterium, Saccharomyces, and Arabidopsis. We compared turboLysis to bead beating using the BeadBug 6 for several concentrations of
Externí odkaz:
https://doaj.org/article/8eb386147ac746f78073ea43b29db2e0
Autor:
Jason D. Limberis, John Z. Metcalfe
Publikováno v:
BMC Bioinformatics, Vol 24, Iss 1, Pp 1-8 (2023)
Abstract Background Multiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of its most important applications is in the targeted sequencing of pathogens. Despite this imp
Externí odkaz:
https://doaj.org/article/96debafe82894f4987a88c0719e5a714
Publikováno v:
PLoS ONE, Vol 18, Iss 11, p e0289442 (2023)
Non-polyadenylated RNA includes a large subset of crucial regulators of RNA expression and constitutes a substantial portion of the transcriptome, playing essential roles in gene regulation. For example, enhancer RNAs are long non-coding RNAs that pe
Externí odkaz:
https://doaj.org/article/f56ffaac10e1489b830d881f08480ce0
Publikováno v:
PLoS ONE, Vol 18, Iss 9, p e0288687 (2023)
Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing, many applications req
Externí odkaz:
https://doaj.org/article/7f7c3e72b95d4d5bb1ed0e3fb9f902a6
Autor:
Sudha Bhadriraju, Douglas W. Fadrosh, Meera K. Shenoy, Din L. Lin, Kole V. Lynch, Kathryn McCauley, Rashida A. Ferrand, Edith D. Majonga, Grace McHugh, Laurence Huang, Susan V. Lynch, John Z. Metcalfe
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
Abstract Chronic lung disease (CLD) is a common co-morbidity for HIV-positive children and adolescents on antiretroviral therapy (ART) in sub-Saharan Africa. In this population, distinct airway microbiota may differentially confer risk of CLD. In a c
Externí odkaz:
https://doaj.org/article/5c889e806c114c2e94ed9a46227d7dc5
Autor:
Nabila Ismail, PhD, Emmanuel Rivière, MSc, Jason Limberis, PhD, Stella Huo, MSc, John Z Metcalfe, ProfPhD, Rob M Warren, ProfPhD, Annelies Van Rie, ProfPhD
Publikováno v:
The Lancet Microbe, Vol 2, Iss 11, Pp e604-e616 (2021)
Summary: Background: Bedaquiline is a crucial drug for control of rifampicin-resistant tuberculosis. Molecular drug resistance assays could facilitate effective use of bedaquiline and surveillance of drug resistance emergence. To facilitate molecular
Externí odkaz:
https://doaj.org/article/fcd16e4bfa3b4da9a5848998efcff179
Autor:
Samuel Akech, Mike English, Thomas G Weiser, Adam D Laytin, Niranjan Kissoon, Jaran Eriksen, Lovenish Bains, Fred Bulamba, Claudia Hanson, Maria Jirwe, Jolene Moore, Mpoki Ulisubisya, Vincent Ioos, Kent Doi, Lee A Wallis, Tom Bashford, Kapil Dev Soni, Nobhojit Roy, Franco Diaz, Mulinda Nyirenda, Nicholas Risko, Hendry R Sawe, Jacob McKnight, Alexandra Wharton-Smith, Jamie Rylance, Monty Khajanchi, Gavin Wooldridge, Emmanuel Fru Nsutebu, Lisa Kurland, Peter Baker, John C Marshall, Kathryn Rowan, Usha Lalla, Balasubramanian Venkatesh, Cornelius Sendagire, Neill KJ Adhikari, Tim Baker, Brian Rice, Josephine Langton, Elisabeth Riviello, Fiona Muttalib, Francis Mupeta, Jacquie Oliwa, Stefan Swartling Peterson, Andrew G Smith, Lorna Guinness, Megan Cox, Wim Van Damme, John Kellett, Elizabeth M Molyneux, Richard Venn, Andrea B Pembe, Mervyn Mer, Ignacio Martin-Loeches, Raymond Towey, Lina Zhang, Blaise Pascal, Hiral A Shah, Carl Otto Schell, Karima Khalid, Paul D Sonenthal, Alex Sanga, Raphael K. Kayambankadzanja, Adam Asghar, Adrian J Holloway, Ahmed Rhassane El Adib, Alexia Michaelides, Alvaro Coronado Munoz, Amos Muzuka, Analía Fernández, Andreas Wellhagen, Anita Gadgil, Anna Hvarfner, Anuja Abayadeera, Asya Agulnik, Aurélie Godard, Bargo Mahamat Yousif, Bhakti Sarang Ben Morton, Bharath Kumar, Tirupakuzhi Vijayaraghavan, Bobby King, C Louise Thwaites, Chian Wern Tai, Christian Owoo, Dan Brun Petersen, Daniel Tatay, David Lee Skinner, Denis Kinyua, Dhruva Ghosh, Diptesh Aryal, Donald Mlombwa, Duyen Thi, Hanh Bui, Edwin R Lugazia, Ellena Heyns, Erika Montalvo, Ernesto Gerardo Moreno, Esther Banda Kanyangira, Furaha Nzanzu, Gibonce Mwakisambwe, Guy A Richards, Hala Ammar, Halinder S Mangat, Hasanein H Ghali, Hoi Ping Shum, Ibrahim Salim Abdullahi, Ingrid T von der Osten, James S Lee, Jane Kasozi Namagga, Jasmine Armour-Marshall, John Z Metcalfe, Jonas Blixt, Juan Gutierrez Mejia, Juan Ignacio Silesky-Jiménez, Karl Martin Kohne, Kazuhiro Yokobatake, Kristina E. Rudd, Kwame Asante Akuamoah-Boateng, Lars Irestedt, Lia I Losonczy, Margaret Nyaika, Markus Castegren, Matthew Loftus, Matti Reinikainen, Michael Jaung, Michael S Lipnick, Miklos Lipcey, Märit Amanda Halmin, Naman Shah, Natalie L Cobb, Nathan D Nielsen, Neville Vlok, Ntogwiachu Daniel Kobuh, Oscar Fernández Rostello, Patricia Duque, Paul Patrick Mwasapi, Petronella Bjurling-Sjöberg, Piedad Sarmiento, Pryanka Relan, Rebecca Silvers, Rehema Mlay, Rich Branson, Richard J Wang, Richard Kojan, Richard Peter Von Rahden, Rob Mac Sweeney, Rodrigo Genaro Arduini, Rodwell Gundo, Ruyumbu Sixtus, Samson Kwazizira Mndolo, Shada A. Rouhani, Siriel Nanzia Massawe, Steven A Webb, Sunkaru Touray, Susana Guido, Teresa Kortz, Theodoros Aslanidis, Traci A Wolbrink, V Theodore Barnett, Vijay Christopher Kannan, Waleed S Eldebsy, Wangari Waweru-Siika, Wezzie Kumwenda Mwafulirwa, William Obeng, Yasein Omer, Zione Banda
Publikováno v:
BMJ Global Health, Vol 6, Iss 9 (2021)
Background Globally, critical illness results in millions of deaths every year. Although many of these deaths are potentially preventable, the basic, life-saving care of critically ill patients are often overlooked in health systems. Essential Emerge
Externí odkaz:
https://doaj.org/article/de5537511f7749b889a3e7d97b1f863c
Autor:
Collins Timire, John Z. Metcalfe, Joconiah Chirenda, Jerod N. Scholten, Barbara Manyame-Murwira, Mkhokheli Ngwenya, Ronnie Matambo, Kelvin Charambira, Herbert Mutunzi, Nico Kalisvaart, Charles Sandy
Publikováno v:
International Journal of Infectious Diseases, Vol 87, Iss , Pp 119-125 (2019)
Objective: To determine the prevalence of resistance to rifampicin alone; rifampicin and isoniazid, and second-line anti-TB drugs among sputum smear-positive tuberculosis patients in Zimbabwe. Design: A health facility-based cross-sectional survey. R
Externí odkaz:
https://doaj.org/article/6d0efa0cd8344c7489d6a4dc5820fc7b
Autor:
Rebecca Tadokera, Stella Huo, Grant Theron, Collins Timire, Salome Manyau-Makumbirofa, John Z Metcalfe
Publikováno v:
PLoS ONE, Vol 16, Iss 7, p e0254204 (2021)
BackgroundDelays in seeking and accessing treatment for rifampicin-resistant tuberculosis (RR-TB) and multi-drug resistant (MDR-TB) are major impediments to TB control in high-burden, resource-limited settings.MethodWe prospectively determined health
Externí odkaz:
https://doaj.org/article/c05c020320f34ccb8c40214e638a31fb
Circularization of rv0678 for Genotypic Bedaquiline Resistance Testing of Mycobacterium tuberculosis
Publikováno v:
Microbiology spectrum, vol 11, iss 2
Circular DNA offers benefits over linear DNA in diagnostic and field assays, but currently, circular DNA generation is lengthy, inefficient, highly dependent on the length and sequence of DNA, and can result in unwanted chimeras. We present streamlin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75a8e7af19dcd759f83aff1a2477ac4c
https://escholarship.org/uc/item/42q5b9gs
https://escholarship.org/uc/item/42q5b9gs