Zobrazeno 1 - 9
of 9
pro vyhledávání: '"John W. Silzel"'
Publikováno v:
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 189
Spectrophotometric titration of buffered solutions of gamma cyclodextrin (γCD) and 1,1′-diethyl,2,2′-dicarbocyanine (DDI) demonstrates extension of the known 1:2 host:guest complex to form a previously unreported 2:2 complex near the γCD solubi
Autor:
John W. Silzel, Katrina Tao Hua Lin
Publikováno v:
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 142
A Franck–Condon (FC) model is used to study the solution-phase absorbance spectra of a series of seven symmetric cyanine dyes having between 22 and 77 atoms. Electronic transition energies were obtained from routine visible-light absorbance and flu
Publikováno v:
Clinical Chemistry. 44:2036-2043
Miniaturization of ligand binding assays may reduce costs by decreasing reagent consumption, but it is less apparent that miniaturized assays can simultaneously exceed the sensitivity of macroscopic techniques by analyte “harvesting” to exploit t
Publikováno v:
Micro Total Analysis Systems ’98 ISBN: 9789401062251
It has been found that 200 micron-diameter spots of covalently immobilized analyte-specific capture reagent can significantly deplete analyte from several hundred microliters of liquid sample. The quantitative ligand binding assays which result sense
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::628eda48fd0410ccc63a8dc1a0acf4a3
https://doi.org/10.1007/978-94-011-5286-0_15
https://doi.org/10.1007/978-94-011-5286-0_15
In this paper, we describe the advantages of utilizing NIR fluors as probe molecules for assays in biological matrices. We describe the development of a sensitive fluorometer capable of sensitive measurements of these molecules and demonstrate the ga
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3fee17ac4ea5cabb616e2533dca18723
https://doi.org/10.1016/s0926-4345(06)80020-9
https://doi.org/10.1016/s0926-4345(06)80020-9
Publikováno v:
SPIE Proceedings.
By confining light from a diode laser within a solid planar waveguide in contact with a liquid sample, an electromagnetic field is produced which extends into the solution for a distance of several hundred nanometers. This `evanescent wave' has all t
Autor:
John W. Silzel, Robert J. Obremski
Publikováno v:
SPIE Proceedings.
Use of the short-wave near infrared (NIR) region for fluorescence spectroscopy is shown to afford enhanced freedom, relative to the visible spectrum, from background signals and noise resulting from scattering and endogenous fluorescence from the sam
Autor:
John W. Silzel, Robert J. Obremski
Publikováno v:
SPIE Proceedings.
Spectrofluorometers employing xenon arc lamp excitation and photomultiplier tube detectors afford sensitivity over the UV/VIS spectral region for which these instruments were designed, but suffer sensitivity limitations in the short-wave near infrare
Autor:
Robert J. Obremski, John W. Silzel
Publikováno v:
Biosensors and Bioelectronics. 13:vi
A system for simultaneously conducting multiple ligand assays on a sample potentially containing target analytes uses as a detector a waveguide having thereon a plurality of probes of known recognition to the target analytes. The probes are in discre