Výsledky vyhledávání - "John VanNice"

Identification in Haloferax volcanii of Phosphomevalonate Decarboxylase and Isopentenyl Phosphate Kinase as Catalysts of the Terminal Enzyme Reactions in an Archaeal Alternate Mevalonate Pathway

Autor: D. A. Skaff, James K. Addo, Andrew Keightley, Henry M. Miziorko, Gerald J. Wyckoff, John VanNice

Publikováno v: Journal of Bacteriology. 196:1055-1063

Mevalonate (MVA) metabolism provides the isoprenoids used in archaeal lipid biosynthesis. In synthesis of isopentenyl diphosphate, the classical MVA pathway involves decarboxylation of mevalonate diphosphate, while an alternate pathway has been propo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd231cd7b405167cf21561d50b243a06
https://doi.org/10.1128/jb.01230-13

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Expression in Haloferax volcanii of 3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase Facilitates Isolation and Characterization of the Active Form of a Key Enzyme Required for Polyisoprenoid Cell Membrane Biosynthesis in Halophilic Archaea

Autor: Henry M. Miziorko, Gerald J. Wyckoff, D. A. Skaff, John VanNice

Publikováno v: Journal of Bacteriology. 195:3854-3862

Enzymes of the isoprenoid biosynthetic pathway in halophilic archaea remain poorly characterized, and parts of the pathway remain cryptic. This situation may be explained, in part, by the difficulty of expressing active, functional recombinant forms

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdc36c4eef040f7b9b488f69237b94ee
https://doi.org/10.1128/jb.00485-13

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Alterations in Ribosomal Protein L19 that Decrease the Fidelity of Translation

Autor: Steven T. Gregory, Divya V. Kamath, John VanNice, Michael O'Connor

Ribosomal protein L19 is an essential ribosomal protein and is a component of bridge B8, one of the protein-RNA bridges linking the large and small ribosomal subunits. Bridge B8 also contributes to the accuracy of translation by affecting GTPase acti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d5e805526ec7d9e5978e5fca13f54de
https://europepmc.org/articles/PMC5859582/

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Identification of H. volcanii Phosphomevalonate Decarboxylase and Isopentenyl Phosphate Kinase as Catalysts of the Terminal Enzymatic Reactions in an Archaeal Alternate Mevalonate Pathway (LB139)

Autor: D. A. Skaff, Andrew Keightley, Henry M. Miziorko, Gerald J. Wyckoff, John VanNice, James K. Addo

Publikováno v: The FASEB Journal. 28

To identify the enzymes responsible for metabolism of mevalonate 5-phosphate to isopentenyl diphosphate in H. volcanii, two open reading frames (HVO_2762; HVO_1412) were selected for expression and characterization. Characterization of these proteins

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b6c4999aaad68065ee3d2e0709bdc72b
https://doi.org/10.1096/fasebj.28.1_supplement.lb139

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