Zobrazeno 1 - 10
of 301
pro vyhledávání: '"John T. Lis"'
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-19 (2023)
Abstract RNA Polymerase II (Pol II) is a multi-subunit complex that undergoes covalent modifications as transcription proceeds through genes and enhancers. Rate-limiting steps of transcription control Pol II recruitment, site and degree of initiation
Externí odkaz:
https://doaj.org/article/6b625f6ad9194754bc249dabd3058a8e
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Abstract Promoter-proximal pausing by RNA Pol II is a rate-determining step in gene transcription that is hypothesized to be a prominent point at which regulatory factors act. The pausing factor NELF is known to induce and stabilize pausing, but not
Externí odkaz:
https://doaj.org/article/a4f397b95a1e4d1ab6d474c1a4c11fb7
Autor:
Jing Luan, Guanjue Xiang, Pablo Aurelio Gómez-García, Jacob M. Tome, Zhe Zhang, Marit W. Vermunt, Haoyue Zhang, Anran Huang, Cheryl A. Keller, Belinda M. Giardine, Yu Zhang, Yemin Lan, John T. Lis, Melike Lakadamyali, Ross C. Hardison, Gerd A. Blobel
Publikováno v:
Cell Reports, Vol 34, Iss 8, Pp 108783- (2021)
Summary: CCCTC-binding factor (CTCF) is a conserved zinc finger transcription factor implicated in a wide range of functions, including genome organization, transcription activation, and elongation. To explore the basis for CTCF functional diversity,
Externí odkaz:
https://doaj.org/article/043972196e1e41779878b3c99b1de8c7
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
While post-translational modifications of the RNA polymerase II complex regulate gene expression, the exact mechanism remains unknown. Here, the authors use altered-specificity kinase mutations to examine the role of Cdk9, Mcs6/Cdk7, and Lsk1/Cdk12 o
Externí odkaz:
https://doaj.org/article/fcb0fae25f044fe193746041c137258a
Autor:
Anniina Vihervaara, Dig Bijay Mahat, Michael J. Guertin, Tinyi Chu, Charles G. Danko, John T. Lis, Lea Sistonen
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-16 (2017)
Heat Shock Factor 1 (HSF1) is a regulator of stress-induced transcription. Here, the authors investigate changes to transcription and chromatin organization upon stress and find that activated HSF1 binds to transcription-primed promoters and enhancer
Externí odkaz:
https://doaj.org/article/8396af8fe6874b93839c2a87662af811
Autor:
Yue Zhao, Qi Liu, Pankaj Acharya, Kristy R. Stengel, Quanhu Sheng, Xiaofan Zhou, Hojoong Kwak, Melissa A. Fischer, James E. Bradner, Stephen A. Strickland, Sanjay R. Mohan, Michael R. Savona, Bryan J. Venters, Ming-Ming Zhou, John T. Lis, Scott W. Hiebert
Publikováno v:
Cell Reports, Vol 16, Iss 7, Pp 2003-2016 (2016)
Bromodomain and extra-terminal domain (BET) family inhibitors offer an approach to treating hematological malignancies. We used precision nuclear run-on transcription sequencing (PRO-seq) to create high-resolution maps of active RNA polymerases acros
Externí odkaz:
https://doaj.org/article/cf9b89b3be554977ab7d018484f46c0c
Autor:
Ian C. Welsh, Hojoong Kwak, Frances L. Chen, Melissa Werner, Lindsay S. Shopland, Charles G. Danko, John T. Lis, Min Zhang, James F. Martin, Natasza A. Kurpios
Publikováno v:
Cell Reports, Vol 13, Iss 2, Pp 337-349 (2015)
Expression of Pitx2 on the left side of the embryo patterns left-right (LR) organs including the dorsal mesentery (DM), whose asymmetric cell behavior directs gut looping. Despite the importance of organ laterality, chromatin-level regulation of Pitx
Externí odkaz:
https://doaj.org/article/307d6acf42cb4ec39f3c4b96c11eb498
Autor:
Isabel X. Wang, Leighton J. Core, Hojoong Kwak, Lauren Brady, Alan Bruzel, Lee McDaniel, Allison L. Richards, Ming Wu, Christopher Grunseich, John T. Lis, Vivian G. Cheung
Publikováno v:
Cell Reports, Vol 6, Iss 5, Pp 906-915 (2014)
RNA sequences are expected to be identical to their corresponding DNA sequences. Here, we found all 12 types of RNA-DNA sequence differences (RDDs) in nascent RNA. Our results show that RDDs begin to occur in RNA chains ∼55 nt from the RNA polymera
Externí odkaz:
https://doaj.org/article/5f2aebfb28ce422f95afdc0f229f309e
Autor:
Colin S. Maxwell, William S. Kruesi, Leighton J. Core, Nicole Kurhanewicz, Colin T. Waters, Caitlin L. Lewarch, Igor Antoshechkin, John T. Lis, Barbara J. Meyer, L. Ryan Baugh
Publikováno v:
Cell Reports, Vol 6, Iss 3, Pp 455-466 (2014)
Fluctuations in nutrient availability profoundly impact gene expression. Previous work revealed postrecruitment regulation of RNA polymerase II (Pol II) during starvation and recovery in Caenorhabditis elegans, suggesting that promoter-proximal pausi
Externí odkaz:
https://doaj.org/article/a2d413ef661c49a3858a72fa6a02b868
Autor:
Leighton J. Core, Joshua J. Waterfall, Daniel A. Gilchrist, David C. Fargo, Hojoong Kwak, Karen Adelman, John T. Lis
Publikováno v:
Cell Reports, Vol 2, Iss 4, Pp 1025-1035 (2012)
Recent genome-wide studies in metazoans have shown that RNA polymerase II (Pol II) accumulates to high densities on many promoters at a rate-limited step in transcription. However, the status of this Pol II remains an area of debate. Here, we compare
Externí odkaz:
https://doaj.org/article/8cc01b03365344349b16149a8bdddb72