Zobrazeno 1 - 10
of 78
pro vyhledávání: '"John T. Stults"'
Autor:
Shawn Novick, Ping Yin, Juliana Kretsinger, Martin H. Gastens, Margaret Ruesch, Ming Zeng, Eileen Berkay, Luca Benetti, John T. Stults, Ann Subashi, Barbara Rellahan, Neha Frantz, Wayne P. Kelley, Corné J.M. Stroop, Laura Pack, David Cirelli, Michael J. Lewis
Publikováno v:
Journal of Pharmaceutical Sciences. 110:771-784
Commercial specifications for a new biotherapeutic product are a critical component of the product's overall control strategy that ensures safety and efficacy. This paper describes strategies for setting commercial specifications as proposed by a con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bec22e712f09017c04b62063741209f5
https://doi.org/10.1016/j.xphs.2020.09.048
https://doi.org/10.1016/j.xphs.2020.09.048
Autor:
David A. Michels, Alice Zhang, John T. Stults, Reed J. Harris, Donald E. Walker, Joseph Carver, Feng Yang, Jeannine Schoenfelder, Delia Li, X. Christopher Yu
Publikováno v:
Analytical Chemistry. 90:13365-13372
Methodologies employing LC-MS/MS have been increasingly used for characterization and identification of residual host cell proteins (HCPs) in biopharmaceutical products to ensure their consistent product quality and safety for patients. To improve th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7357a22c743a89302c706a5ceca5e4a5
https://doi.org/10.1021/acs.analchem.8b03044
https://doi.org/10.1021/acs.analchem.8b03044
Autor:
John T. Stults, Hans-Martin Mueller, Yan Wang, Aparna Deora, John F. Valliere-Douglass, Sampath R. Kumar, Sam Yaghmour, Lisa A. Marzilli, Charles Nwosu, Luhong He, Zhimei Du, Yan-Hui Liu, Yizhou Zhou
Publikováno v:
PDA journal of pharmaceutical science and technology. 73(6)
The application of advanced methodologies such as next-generation sequencing (NGS) and mass spectrometry (MS) to the characterization of cell lines and recombinant proteins has enabled the highly sensitive detection of sequence variants (SVs). Howeve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74a2c3250d8067934b862809adc58d41
https://pubmed.ncbi.nlm.nih.gov/31209169
https://pubmed.ncbi.nlm.nih.gov/31209169
Publikováno v:
Pharmaceutical Research. 30:167-178
To examine and determine the sites and the kinetics of IgG1 mAb modifications from both in vitro (rat plasma and PBS) and in vivo (rat model) time-course studies. A comprehensive set of protein characterization methods, including RPLC/MS, LC-MS/MS, i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25d455251869451c91ca44fa401b64ad
https://doi.org/10.1007/s11095-012-0860-z
https://doi.org/10.1007/s11095-012-0860-z
Autor:
Guillaume Tremintin, Dell Farnan, George Tony Moreno, John T. Stults, Mark van Gils, Andreas Becker
Publikováno v:
Journal of Chromatography A. 1216:8904-8909
Size exclusion chromatography is a widely performed analysis of monoclonal antibodies, primarily used to monitor the levels of higher weight molecular species such as aggregates. Owing to the subtleties of these separation mechanisms and frequently o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91e743941f3162fe22ddf01389c3c857
https://doi.org/10.1016/j.chroma.2009.10.045
https://doi.org/10.1016/j.chroma.2009.10.045
Publikováno v:
Journal of Immunological Methods. 341:59-67
Resins containing immobilized Staphylococcal Protein A (PA) are widely used in the commercial purification of recombinant human monoclonal antibody (rhuMAb IgG) biotherapeutics. Therefore, a sensitive assay for leached PA is needed to ensure that PA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5816e415ab23d8f97d18469841d52fde
https://doi.org/10.1016/j.jim.2008.10.015
https://doi.org/10.1016/j.jim.2008.10.015
Publikováno v:
Molecular & Cellular Proteomics. 4:235-245
Reversible phosphorylation of proteins is among the most important post-translational modifications, and elucidation of sites of phosphorylation is essential to understanding the regulation of key cellular processes such as signal transduction. Unfor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6d15451a6523e5ce1382400e7c320c38
https://doi.org/10.1074/mcp.r400011-mcp200
https://doi.org/10.1074/mcp.r400011-mcp200
Autor:
Kevin R Clark, Thomas R. Gadek, Martin E. Struble, James H. Bourell, Denise A. Ockey, Jenna Dotson, John T. Stults
Publikováno v:
Bioorganic & Medicinal Chemistry. 12:37-44
A novel class of nonpeptide inhibitors of stromelysin (MMP-3) has been discovered with the use of mass spectrometry. The method relies on the development of structure–activity relationships by mass spectrometry (SAR by MS) and utilizes information
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b66b0c91c5bac46b85e9ceadce0c11c
https://doi.org/10.1016/j.bmc.2003.10.053
https://doi.org/10.1016/j.bmc.2003.10.053
Autor:
John T. Stults, Adrianne Kishiyama, David Arnott, James C. Marsters, Sarah G. Ludlum, Elizabeth Luis
Publikováno v:
Molecular & Cellular Proteomics. 1:148-156
A method has been developed, called the mass western experiment in analogy to the Western blot, to detect the presence of specific proteins in complex mixtures without the need for antibodies. Proteins are identified with high sensitivity and selecti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e9dc929f8ecf46ce1e127c62ff0009ca
https://doi.org/10.1074/mcp.m100027-mcp200
https://doi.org/10.1074/mcp.m100027-mcp200