Zobrazeno 1 - 10
of 18
pro vyhledávání: '"John Richard Nelson"'
Autor:
Erin Finehout, Adrian V. Lee, Shannon Puhalla, Erik Leeming Kvam, Nancy E. Davidson, Karthik Kota, Weston Blaine Griffin, Rekha Gyanchandani, Nicholas G. Smith, Ryan Charles Heller, John Richard Nelson, Adam Brufsky
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
Scientific Reports
Scientific Reports
The ability to measure mutations in plasma cell-free DNA (cfDNA) has the potential to revolutionize cancer surveillance and treatment by enabling longitudinal monitoring not possible with solid tumor biopsies. However, obtaining sufficient quantities
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b19f67bc24d299c2db749dbce74327ba
https://doi.org/10.1038/s41598-018-35470-9
https://doi.org/10.1038/s41598-018-35470-9
Autor:
Christine Surrette, David Shoudy, Wei Gao, Todd Frederick Miller, Maria I. Zavodszky, Nichole L. Wood, Stanislav L. Karsten, Chris Puleo, Corwin Alex D, Michael J. Gerdes, John Richard Nelson
Publikováno v:
SLAS technology. 22(4)
We present a mesodissection platform that retains the advantages of laser-based dissection instrumentation with the speed and ease of manual dissection. Tissue dissection in clinical laboratories is often performed by manually scraping a physician-se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35b2678f27becf34919a33816cca13b8
https://pubmed.ncbi.nlm.nih.gov/27864340
https://pubmed.ncbi.nlm.nih.gov/27864340
Autor:
Andre R. Arellano, Yunian Lou, Paul G. Richardson, John C. Detter, Mei Wang, Eileen Dalin, Jamie Jett, John Richard Nelson, Trevor Hawkins, Jarrod Chapman, Susan Lucas, Daniel S. Rokhsar
Publikováno v:
Genomics. 80:691-698
Amplification of source DNA is a nearly universal requirement for molecular biology applications. The primary methods currently available to researchers are limited to in vivo amplification in Escherichia coli hosts and the polymerase chain reaction.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aaf7b931dc86ac93aa51eecac683ddc4
https://doi.org/10.1006/geno.2002.7020
https://doi.org/10.1006/geno.2002.7020
Autor:
Yuyang Christine Cai, J. Anthony Mamone, Theresa L. Giesler, Joseph W. Farchaus, Chockalingam Palaniappan, Maria Ortiz-Rivera, John Richard Nelson, Lou P. Hosta, Peter L. Hewitt, Shanmuuga Sundaram, Carl W. Fuller
Publikováno v:
BioTechniques. 32:S44-S47
We have developed a novel, isothermal DNA amplification strategy that employs ⌽29 DNA polymerase and rolling circle amplification to generate high-quality templates for DNA sequencing reactions. The TempliPhi™ DNA amplification kits take advantag
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5fd565f9941381c553dcf810c87d7621
https://doi.org/10.2144/jun0208
https://doi.org/10.2144/jun0208
Autor:
John Richard Nelson
Publikováno v:
Current Protocols in Molecular Biology
Whole-genome amplification by multiple displacement amplification (MDA) is a patented method to generate potentially unlimited genomic material when researchers are challenged with trace samples, or the amount of genomic DNA required for analysis exc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e6ad60fcef8a4770a5dbaef1f68f2d9
https://doi.org/10.1002/0471142727.mb1513s105
https://doi.org/10.1002/0471142727.mb1513s105
Autor:
Mahesh Khot, Satyam Nampalli, John Richard Nelson, Carl W. Fuller, Shiv Kumar, Parke K. Flick
Publikováno v:
ChemInform. 32
Fluorescence resonance energy transfer (FRET) based dye-nucleotide terminators (10–13) were designed, synthesized, and formulated with Thermo Sequenase™ II DNA polymerase into a robust kit for high throughput DNA sequencing. The key energy transf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3ca18c7687e72413c4c10cf8c275ee24
https://doi.org/10.1002/chin.200147200
https://doi.org/10.1002/chin.200147200
Autor:
Kate Lavrenov, Satyam Nampalli, T. Sudhakar Rao, Haiguang Xiao, Shiv Kumar, Weihong Zhang, John Richard Nelson
Publikováno v:
ChemInform. 32
The synthesis of four color set of energy transfer-dye terminators (8a–8d) starting from p-iodo-β-phenylalanine was accomplished and their utility in the sequencing reactions has been evaluated.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a79e980afd3151cb067d380912bee8ba
https://doi.org/10.1002/chin.200147220
https://doi.org/10.1002/chin.200147220
Autor:
Steven A. Benner, John Richard Nelson, Timothy D. Harris, Carl W. Fuller, Dmitri V. Vezenov, David C. Schwartz, Stevan B Jovanovich, George M. Church, Xiaohua Huang, Lyle Richard Middendorf, Jeffery A. Schloss
Publikováno v:
Nature biotechnology. 27(11)
DNA sequencing-by-synthesis (SBS) technology, using a polymerase or ligase enzyme as its core biochemistry, has already been incorporated in several second-generation DNA sequencing systems with significant performance. Notwithstanding the substantia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88ca0d85f90b8af66765ed34f4c49179
https://pubmed.ncbi.nlm.nih.gov/19898456
https://pubmed.ncbi.nlm.nih.gov/19898456
Autor:
Jeffery Wegener, Anuradha Sekher, Carl W. Fuller, Satyam Nampalli, John Richard Nelson, John Macklin, Shiv Kumar, Patrick J. Finn, Anup Sood, Paul Mitsis
Publikováno v:
Nucleosides, nucleotidesnucleic acids. 24(5-7)
A number of terminal phosphate-labeled nucleotides with three or more phosphates and with varied length linkers attached between the terminal phosphate and the dye have been synthesized. These nucleotides have been tested as substrates for different
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24c71b78ddb4b6311cc62522b65cd773
https://pubmed.ncbi.nlm.nih.gov/16247959
https://pubmed.ncbi.nlm.nih.gov/16247959
Autor:
Bernard F. Mcardle, Greg Grossmann, Matthew G. Bull, Haiguang Xiao, J. Anthony Mamone, Carl W. Fuller, Satyam Nampalli, Parke K. Flick, John Richard Nelson, Shiv Kumar, Patrick J. Finn, Paula D. Phillips
Publikováno v:
Nucleic acids research. 31(16)
A series of charge-modified, dye-labeled 2′, 3′-dideoxynucleoside-5′-triphosphates have been synthesized and evaluated as reagents for dye-terminator DNA sequencing. Unlike the commonly used dye-labeled terminators, these terminators possess a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdcd946647528ea49b69f69169fc4388
https://pubmed.ncbi.nlm.nih.gov/12907718
https://pubmed.ncbi.nlm.nih.gov/12907718