Zobrazeno 1 - 6
of 6
pro vyhledávání: '"John R. Dobrinsky"'
Autor:
John R. Garbe, Wenfang Tan, Karl J. Clark, John R. Dobrinsky, Perry B. Hackett, Michael J. Martin, Daniel F. Carlson, Scott C. Fahrenkrug
Publikováno v:
Transgenic Research. 20:1125-1137
Swine transgenesis by pronuclear injection or cloning has traditionally relied on illegitimate recombination of DNA into the pig genome. This often results in animals containing concatemeric arrays of transgenes that complicate characterization and c
Publikováno v:
Biology of Reproduction. 47:960-969
Sperm-induced calcium (Ca2+) changes were examined in zona pellucida-intact, mature bovine eggs injected with the fluorescent Ca2+ indicator fura-2 dextran (fura-2 D). Fifty four percent (37/68) of the dye-injected, inseminated bovine eggs were ferti
Autor:
Jie, Zhu, Evelyn E, Telfer, Judy, Fletcher, Anthea, Springbett, John R, Dobrinsky, Paul A, De Sousa, Ian, Wilmut
Publikováno v:
Biology of reproduction. 66(3)
Factors influencing pig oocyte activation by electrical stimulation were evaluated by their effect on the development of parthenogenetic embryos to the blastocyst stage to establish an effective activation protocol for pig nuclear transfer. This eval
Autor:
Paul A, De Sousa, John R, Dobrinsky, Jie, Zhu, Alan L, Archibald, Alison, Ainslie, Wim, Bosma, June, Bowering, John, Bracken, Patricia M, Ferrier, Judy, Fletcher, Bianca, Gasparrini, Linda, Harkness, Paul, Johnston, Marjorie, Ritchie, William A, Ritchie, Ailsa, Travers, David, Albertini, Andras, Dinnyes, Timothy J, King, Ian, Wilmut
Publikováno v:
Biology of reproduction. 66(3)
To clone a pig from somatic cells, we first validated an electrical activation method for use on ovulated oocytes. We then evaluated delayed versus simultaneous activation (DA vs. SA) strategies, the use of 2 nuclear donor cells, and the use of cytos
Autor:
Franklin D. West, Steve L. Terlouw, Dae Jin Kwon, Jennifer L. Mumaw, Sujoy K. Dhara, Kowser Hasneen, John R. Dobrinsky, Steven L. Stice
Publikováno v:
Stem Cells & Development; Aug2010, Vol. 19 Issue 8, p1211-1220, 10p