N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry

Autor: Dongxia Wang, Bin Zhou, Theodore R. Keppel, Maria Solano, Jakub Baudys, Jason Goldstein, M. G. Finn, Xiaoyu Fan, Asheley P. Chapman, Jonathan L. Bundy, Adrian R. Woolfitt, Sarah H. Osman, James L. Pirkle, David E. Wentworth, John R. Barr

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)

Abstract N-glycosylation plays an important role in the structure and function of membrane and secreted proteins. The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is

Externí odkaz: https://doaj.org/article/94a2b1bf24dc4c3b9e141f9e202394e4

Comprehensive characterization of toxins during progression of inhalation anthrax in a non-human primate model.

Autor: Anne E Boyer, Maribel Gallegos-Candela, Renato C Lins, Maria I Solano, Adrian R Woolfitt, John S Lee, Daniel C Sanford, Katherine A B Knostman, Conrad P Quinn, Alex R Hoffmaster, James L Pirkle, John R Barr

Publikováno v: PLoS Pathogens, Vol 18, Iss 12, p e1010735 (2022)

Inhalation anthrax has three clinical stages: early-prodromal, intermediate-progressive, and late-fulminant. We report the comprehensive characterization of anthrax toxins, including total protective antigen (PA), total lethal factor (LF), total edem

Externí odkaz: https://doaj.org/article/e29878e29f4a4d4bab71c0fda04880e9

Integrated Quantitative Targeted Lipidomics and Proteomics Reveal Unique Fingerprints of Multiple Metabolic Conditions

Autor: Anna A. Ivanova, Jon C. Rees, Bryan A. Parks, Michael Andrews, Michael Gardner, Eunice Grigorutsa, Zsuzsanna Kuklenyik, James L. Pirkle, John R. Barr

Publikováno v: Biomolecules, Vol 12, Iss 10, p 1439 (2022)

Aberrations in lipid and lipoprotein metabolic pathways can lead to numerous diseases, including cardiovascular disease, diabetes, neurological disorders, and cancer. The integration of quantitative lipid and lipoprotein profiling of human plasma may

Externí odkaz: https://doaj.org/article/e9636a77d1b842bda0cb1ae2beceece2

Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950–Metabolites in Frozen Human Plasma[S]

Autor: John A. Bowden, Alan Heckert, Candice Z. Ulmer, Christina M. Jones, Jeremy P. Koelmel, Laila Abdullah, Linda Ahonen, Yazen Alnouti, Aaron M. Armando, John M. Asara, Takeshi Bamba, John R. Barr, Jonas Bergquist, Christoph H. Borchers, Joost Brandsma, Susanne B. Breitkopf, Tomas Cajka, Amaury Cazenave-Gassiot, Antonio Checa, Michelle A. Cinel, Romain A. Colas, Serge Cremers, Edward A. Dennis, James E. Evans, Alexander Fauland, Oliver Fiehn, Michael S. Gardner, Timothy J. Garrett, Katherine H. Gotlinger, Jun Han, Yingying Huang, Aveline Huipeng Neo, Tuulia Hyötyläinen, Yoshihiro Izumi, Hongfeng Jiang, Houli Jiang, Jiang Jiang, Maureen Kachman, Reiko Kiyonami, Kristaps Klavins, Christian Klose, Harald C. Köfeler, Johan Kolmert, Therese Koal, Grielof Koster, Zsuzsanna Kuklenyik, Irwin J. Kurland, Michael Leadley, Karen Lin, Krishna Rao Maddipati, Danielle McDougall, Peter J. Meikle, Natalie A. Mellett, Cian Monnin, M. Arthur Moseley, Renu Nandakumar, Matej Oresic, Rainey Patterson, David Peake, Jason S. Pierce, Martin Post, Anthony D. Postle, Rebecca Pugh, Yunping Qiu, Oswald Quehenberger, Parsram Ramrup, Jon Rees, Barbara Rembiesa, Denis Reynaud, Mary R. Roth, Susanne Sales, Kai Schuhmann, Michal Laniado Schwartzman, Charles N. Serhan, Andrej Shevchenko, Stephen E. Somerville, Lisa St. John-Williams, Michal A. Surma, Hiroaki Takeda, Rhishikesh Thakare, J. Will Thompson, Federico Torta, Alexander Triebl, Martin Trötzmüller, S. J. Kumari Ubhayasekera, Dajana Vuckovic, Jacquelyn M. Weir, Ruth Welti, Markus R. Wenk, Craig E. Wheelock, Libin Yao, Min Yuan, Xueqing Heather Zhao, Senlin Zhou

Publikováno v: Journal of Lipid Research, Vol 58, Iss 12, Pp 2275-2288 (2017)

As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950–Metabolites in Frozen Human Plasma

Externí odkaz: https://doaj.org/article/e757670a32e84af9bc9bad52c975b5dc

Confirmation of Statin and Fibrate Use from Small-Volume Archived Plasma Samples by High-Throughput LC-MS/MS Method

Autor: Jennifer D. Kusovschi, Anna A. Ivanova, Michael S. Gardner, Robert W. McGarrah, William E. Kraus, Zsuzsanna Kuklenyik, James L. Pirkle, John R. Barr

Publikováno v: International Journal of Molecular Sciences; Volume 24; Issue 9; Pages: 7931

Designing studies for lipid-metabolism-related biomarker discovery is challenging because of the high prevalence of various statin and fibrate usage for lipid-lowering therapies. When the statin and fibrate use is determined based on self-reports, pa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6198016ec2e7a658578dd0d3bb8d6ff3