Zobrazeno 1 - 10
of 28
pro vyhledávání: '"John Petruska"'
Autor:
Myron F. Goodman, John Petruska
Publikováno v:
Nature reviews. Chemistry. 1(9)
Controversy surrounds the perceived absence of a relationship between DNA polymerase fidelity (kinetic discrimination) and free energy changes determined from DNA melting studies (thermodynamic discrimination). Thermodynamic discrimination together w
Autor:
Laura M. Barge, John Petruska
Publikováno v:
Chemical Physics Letters. 556:315-319
Reactions controlled by diffusion create self-organizing periodic patterns in chemical systems and play a key role in biological pattern formation through diffusion-generated morphogen gradients. To better understand the organizing ability of diffusi
Autor:
Eric T. Kool, Emily M. Harcourt, Michael G. Mohsen, John Petruska, Keriann Oertell, Myron F. Goodman
Publikováno v:
Proceedings of the National Academy of Sciences. 113
What is the free energy source enabling high-fidelity DNA polymerases (pols) to favor incorporation of correct over incorrect base pairs by 10(3)- to 10(4)-fold, corresponding to free energy differences of ΔΔGinc∼ 5.5-7 kcal/mol? Standard ΔΔG°
Autor:
Laura M. Barge, Kenneth H. Nealson, S. L. Potter, Marjorie A. Chan, Douglas E. Hammond, John Petruska
Publikováno v:
Geofluids. 11:124-133
We compare laboratory and field examples of self-organized mineral precipitates in porous media. Laboratorytests of silver chromate precipitation in glass beads and glass bead⁄gel mixtures produce structures such as peri-odic banding and mm-size s
Publikováno v:
Chemical Physics Letters. 493:340-345
The well-known AgNO 3 /K 2 CrO 4 reaction–diffusion system produces periodic bands of silver chromate precipitate in gelatin, but only randomly oriented crystals in agarose gel. We show that comparable bands can be produced in agarose gel by adding
Publikováno v:
Biochemistry. 49:20-28
DNA polymerase fidelity is defined as the ratio of right (R) to wrong (W) nucleotide incorporations when dRTP and dWTP substrates compete at equal concentrations for primer extension at the same site in the polymerase-primer-template DNA complex. Typ
Publikováno v:
Journal of Biological Chemistry. 284:27761-27765
Activation-induced cytidine deaminase (AID) and APOBEC3G catalyze deamination of cytosine to uracil on single-stranded DNA, thereby setting in motion a regulated hypermutagenic process essential for human well-being. However, if regulation fails, hav
Publikováno v:
Journal of Biological Chemistry. 277:41379-41389
Using synthetic DNA constructs in vitro, we find that human DNA polymerase beta effectively catalyzes CAG/CTG triplet repeat expansions by slippage initiated at nicks or 1-base gaps within short (14 triplet) repeat tracts in DNA duplexes under physio
Publikováno v:
Journal of Biological Chemistry. 275:18382-18390
Repetitions of CAG or CTG triplets in DNA can form intrastrand hairpin loops with combinations of normal and mismatched base pairs that easily rearrange. Such loops may promote primer-template slippage in DNA replication or repair to give triplet-rep
Publikováno v:
Journal of Biological Chemistry. 273:5204-5210
Lengthy expansions of trinucleotide repeats are found in DNA of patients suffering severe neurodegenerative age-related diseases. Using a synthetic self-priming DNA, containing CAG and CTG repeats implicated in Huntington's disease and several other