Zobrazeno 1 - 10
of 11
pro vyhledávání: '"John Peter Rickgauer"'
Publikováno v:
Nature Neuroscience. 17:1816-1824
Linking neural microcircuit function to emergent properties of the mammalian brain requires fine-scale manipulation and measurement of neural activity during behavior, where each neuron’s coding and dynamics can be characterized. We developed an op
Autor:
Dwight L. Anderson, Paul J. Jardine, John Peter Rickgauer, Shelley Grimes, Douglas E. Smith, Derek N. Fuller
Publikováno v:
Proceedings of the National Academy of Sciences. 104:11245-11250
In many viruses, DNA is confined at such high density that its bending rigidity and electrostatic self-repulsion present a strong energy barrier in viral assembly. Therefore, a powerful molecular motor is needed to package the DNA into the viral caps
Autor:
John Peter Rickgauer, David W. Tank
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 106(35)
We demonstrate that channelrhodopsin-2 (CR), a light-gated ion channel that is conventionally activated by using visible-light excitation, can also be activated by using IR two-photon excitation (TPE). An empirical estimate of CR's two-photon absorpt
Autor:
Shelley Grimes, Douglas E. Smith, Dorian M. Raymer, Dwight L. Anderson, John Peter Rickgauer, Carlos Enrique Catalano, Rae M. Robertson, Derek N. Fuller
Publikováno v:
Journal of molecular biology. 373(5)
Molecular motors drive genome packaging into preformed procapsids in many double-stranded (ds)DNA viruses. Here, we present optical tweezers measurements of single DNA molecule packaging in bacteriophage lambda. DNA-gpA-gpNu1 complexes were assembled
Autor:
Paul J. Jardine, Dwight L. Anderson, Derek N. Fuller, Shelley Grimes, John Peter Rickgauer, Douglas E. Smith
Publikováno v:
SPIE Proceedings.
The bacteriophage φ29 portal motor is capable of packaging the φ29, 19.3 Kbp, genome to high density into its preformed capsid. The packaging process must overcome the forces due to confining the highly negative charge of the DNA to a small volume,
Autor:
John Peter Rickgauer, Douglas E. Smith, Paul J. Jardine, Derek N. Fuller, Shelley Grimes, Dwight L. Anderson, Bo Hu
Publikováno v:
SPIE Proceedings.
Autor:
John Peter Rickgauer, Shelley Grimes, Derek N. Fuller, Paul J. Jardine, Dwight L. Anderson, Douglas E. Smith
Publikováno v:
The FASEB Journal. 20
Autor:
Derek N. Fuller, Gregory J. Gemmen, John Peter Rickgauer, Douglas E. Smith, Rachel Millin, Allan L. Schweitzer
Publikováno v:
SPIE Proceedings.
Mechanical manipulation of single DNA molecules can provide novel information about protein-DNA interactions. Here we review two examples studied by our group. First, we have studied the forced unraveling of nucleosomes assembled on heterogeneous DNA
Autor:
Gregory J. Gemmen, Pierre Recouvreux, Douglas E. Smith, Derek N. Fuller, Rachel Millin, John Peter Rickgauer, Aurélie Dupont
Publikováno v:
Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2005, 34 (2), pp.e15
Nucleic Acids Research, 2005, 34 (2), pp.e15
Nucleic Acids Research, Oxford University Press, 2005, 34 (2), pp.e15
Nucleic Acids Research, 2005, 34 (2), pp.e15
International audience; Mechanical manipulation of single DNA molecules can provide novel information about DNA properties and protein-DNA interactions. Here we describe and characterize a useful method for manipulating desired DNA sequences from any
Publikováno v:
Biophysical Journal. (11):4253-4257
Optical tweezers have broad applications in studies of structures and processes in molecular and cellular biophysics. Use of optical tweezers for quantitative molecular-scale measurement requires careful calibration in physical units. Here we show th