Zobrazeno 1 - 10 of 27 pro vyhledávání: '"John P. McGee"'
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Akademický článek
Automated imaging and identification of proteoforms directly from ovarian cancer tissue
Autor: John P. McGee, Pei Su, Kenneth R. Durbin, Michael A. R. Hollas, Nicholas W. Bateman, G. Larry Maxwell, Thomas P. Conrads, Ryan T. Fellers, Rafael D. Melani, Jeannie M. Camarillo, Jared O. Kafader, Neil L. Kelleher
Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-11 (2023)
Abstract The molecular identification of tissue proteoforms by top-down mass spectrometry (TDMS) is significantly limited by throughput and dynamic range. We introduce AutoPiMS, a single-ion MS based multiplexed workflow for top-down tandem MS (MS2)
Externí odkaz: https://doaj.org/article/7587327dde1148caba435a60d10f292c
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3
Native Mass Spectrometry at the Convergence of Structural Biology and Compositional Proteomics
Autor: Kevin Jooß, John P. McGee, Neil L. Kelleher
Publikováno v: Accounts of chemical research. 55(14)
Biology is driven by a vast set of molecular interactions that evolved over billions of years. Just as covalent modifications like acetylations and phosphorylations can change a protein's function, so too can noncovalent interactions with metals, sma
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22d7da3622e779a1476601ae3c828cc2
https://pubmed.ncbi.nlm.nih.gov/35749283
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4
Automated Control of Injection Times for Unattended Acquisition of Multiplexed Individual Ion Mass Spectra
Autor: John P. McGee, Michael W. Senko, Kevin Jooß, Benjamin J. Des Soye, Philip D. Compton, Neil L. Kelleher, Jared O. Kafader
Publikováno v: Analytical chemistry. 94(48)
Charge detection mass spectrometry (CDMS) provides mass domain spectra of large and highly heterogeneous analytes. Over the past few years, we have multiplexed CDMS on Orbitrap instruments, an approach termed Individual Ion Mass Spectrometry (I
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6137bf6daff116ce7d2d6701f37183cd
https://pubmed.ncbi.nlm.nih.gov/36416365
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5
Highly multiplexed, label-free proteoform imaging of tissues by individual ion mass spectrometry
Autor: Pei Su, John P. McGee, Kenneth R. Durbin, Michael A. R. Hollas, Manxi Yang, Elizabeth K. Neumann, Jamie L. Allen, Bryon S. Drown, Fatma Ayaloglu Butun, Joseph B. Greer, Bryan P. Early, Ryan T. Fellers, Jeffrey M. Spraggins, Julia Laskin, Jeannie M. Camarillo, Jared O. Kafader, Neil L. Kelleher
Publikováno v: Science advances. 8(32)
Imaging of proteoforms in human tissues is hindered by low molecular specificity and limited proteome coverage. Here, we introduce proteoform imaging mass spectrometry (PiMS), which increases the size limit for proteoform detection and identification
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17b4e257e35227291c9bd87d3f9a773c
https://pubmed.ncbi.nlm.nih.gov/35947651
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6
Isotopic Resolution of Protein Complexes up to 466 kDa Using Individual Ion Mass Spectrometry
Autor: Michael W. Senko, Ping Yip, Neil L. Kelleher, Rafael D. Melani, Philip D. Compton, John P. McGee, Jared O. Kafader
Publikováno v: Anal Chem
Native mass spectrometry involves transferring large biomolecular complexes into the gas phase, enabling the characterization of their composition and stoichiometry. However, the overlap in distributions created by residual solvation, ionic adducts,
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95a46edc5532cfc8bc6ff6f74b6c1657
https://doi.org/10.1021/acs.analchem.0c03282
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7
Voltage Rollercoaster Filtering of Low-Mass Contaminants During Native Protein Analysis
Autor: John P. McGee, Rafael D. Melani, Neil L. Kelleher, Philip D. Compton, Romain Huguet, Michael P. Goodwin, Graeme C. McAlister, Michael W. Senko
Publikováno v: J Am Soc Mass Spectrom
Intact protein mass spectrometry (MS) via electrospray-based methods is often degraded by low-mass contaminants, which can suppress the spectral quality of the analyte of interest via space-charge effects. Consequently, selective removal of contamina
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::925865953cdeec0b7cd86d9a7deafad6
https://doi.org/10.1021/jasms.9b00037
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