Zobrazeno 1 - 10
of 15
pro vyhledávání: '"John P. Hachmann"'
Autor:
Michal Lebl, Anu Srinivasan, Igor A. Kozlov, Melissa D. Shults, Nicholas A. Nelson, David L. Barker, John P. Hachmann, Peter C. Melnyk, Chanfeng Zhao, Joseph Musmacker
Publikováno v:
Combinatorial Chemistry & High Throughput Screening. 11:24-35
We have developed a miniaturized and multiplexed solution assay for the measurement of protease activity in complex samples. This technology can accelerate research in functional proteomics and enable biologists to carry out multiplexed protease inhi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b3c641ef547b5d55928d3d5439b899d
https://doi.org/10.2174/138620708783398304
https://doi.org/10.2174/138620708783398304
Autor:
Peter C. Melnyk, Chanfeng Zhao, Igor A. Kozlov, Michal Lebl, John P. Hachmann, David L. Barker
Publikováno v:
Nucleosides, Nucleotides and Nucleic Acids. 26:1353-1357
We developed novel assays for high-throughput detection of one or many kinases or proteases. The assays use hundreds of different peptide substrates, each covalently linked to an oligonucleotide tag. After incubation with sample, the pool of substrat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3e5cd3a214d2212d0d19adfc4c18b8f
https://doi.org/10.1080/15257770701533909
https://doi.org/10.1080/15257770701533909
Autor:
Igor A. Kozlov, Michal Lebl, Chanfeng Zhao, David L. Barker, Peter C. Melnyk, John P. Hachmann, Bahram Ghaffarzadeh Kermani
Publikováno v:
Sensors and Actuators B: Chemical. 125:149-157
Retention of histidine-containing peptides in immobilized metal-affinity chromatography (IMAC) has been studied using several hundred model peptides. Retention in a Nickel column is primarily driven by the number of histidine residues; however, the a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44d9750fe7585465187d25548cd02b5a
https://doi.org/10.1016/j.snb.2007.02.004
https://doi.org/10.1016/j.snb.2007.02.004
Autor:
Karel Zenisek, Vaclav Pesek, Pavel Poncar, Christine Pistek, Michal Lebl, John P. Hachmann, Petr Mudra, Vit Pokorny
Publikováno v:
International Journal of Peptide Research and Therapeutics. 13:367-375
We have developed a small benchtop oligonucleotide synthesizer which allows the scientist to prepare, rapidly and economically, up to 24 oligonucleotides in one batch. We have shown that this instrument can be used for peptide synthesis, as well. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::245e3093b52179c80d29e86deda44479
https://doi.org/10.1007/s10989-007-9096-x
https://doi.org/10.1007/s10989-007-9096-x
Autor:
John P. Hachmann, Veronika Shevchenko, Igor A. Kozlov, Peter C. Melnyk, Chanfeng Zhao, Michal Lebl, Anu Srinivasan, David L. Barker
Publikováno v:
Combinatorial Chemistry & High Throughput Screening. 9:481-487
We have developed a high throughput assay for the measurement of protease activity in solution. This technology will accelerate research in functional proteomics and enable biologists to streamline protease substrate evaluation and optimization. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6311d6401a5bf7a67b634346a88f216
https://doi.org/10.2174/138620706777698535
https://doi.org/10.2174/138620706777698535
Autor:
Michal Lebl, John P. Hachmann
Publikováno v:
Biopolymers. 84:340-347
Ten different coupling reagents and their combinations were tested in parallel in the synthesis of four model peptide sequences. Significant differences were found between uronium and phosphonium salt-based reagents and carbodiimide. Diisopropylcarbo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e75440eabaf89db3a1740328aeb19d3e
https://doi.org/10.1002/bip.20482
https://doi.org/10.1002/bip.20482
Autor:
Michal Lebl, David L. Barker, Chanfeng Zhao, John P. Hachmann, Peter C. Melnyk, Igor A. Kozlov, Bahram Ghaffarzadeh Kermani
Publikováno v:
Journal of chromatographic science. 45(4)
The retention of histidine-containing peptides in immobilized metal-affinity chromatography is studied using several hundred modeled peptides. Retention is driven primarily by the number of histidine residues; however, the amino acid composition in t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7b25cc877c6e47ea2bb4b667a501573
https://pubmed.ncbi.nlm.nih.gov/17504569
https://pubmed.ncbi.nlm.nih.gov/17504569
Autor:
Veronika Shevchenko, Peter C. Melnyk, Chanfeng Zhao, Joseph Musmacker, Bahram Ghaffarzadeh Kermani, Nicholas A. Nelson, Melissa D. Shults, Michal Lebl, John P. Hachmann, David L. Barker, Igor A. Kozlov, Anu Srinivasan
Publikováno v:
Chembiochem : a European journal of chemical biology. 8(8)
We report a novel protein kinase assay designed for high-throughput detection of one or many kinases in a complex mixture. A solution-phase phosphorylation reaction is performed on 900 different peptide substrates, each covalently linked to an oligon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a544cc6130882d72414f282e4a847f2
https://pubmed.ncbi.nlm.nih.gov/17471478
https://pubmed.ncbi.nlm.nih.gov/17471478
Autor:
Michal Lebl, John P. Hachmann
Publikováno v:
Nucleosides, nucleotidesnucleic acids. 25(7)
Model sequences for evaluation of the GC dimer sequence repetition on synthesis success were prepared and analyzed by HPLC. Contiguous d(G-C) or d(C-G) sequences have a deleterious effect on DNA oligonucleotide synthesis. The critical number seems to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c35d2d81ca3658aeae0d394d3fbf94d4
https://pubmed.ncbi.nlm.nih.gov/16898410
https://pubmed.ncbi.nlm.nih.gov/16898410
Autor:
Michal Lebl, John P. Hachmann
Publikováno v:
Journal of combinatorial chemistry. 8(2)
4-Methylpiperidine is fully equivalent to piperidine in removal of Fmoc protecting group in solid phase synthesis. This reagent is not a controlled substance and its use does not require any paperwork.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97c4d764452ff895a4616f83bf1b8df3
https://pubmed.ncbi.nlm.nih.gov/16529506
https://pubmed.ncbi.nlm.nih.gov/16529506