Zobrazeno 1 - 10
of 15
pro vyhledávání: '"John L. Armstrong"'
Autor:
L. A. Porteous, John L. Armstrong
Publikováno v:
Current Microbiology. 27:115-118
A rapid, simple method is used that yields amplifiable fungal and bacterial DNAs directly from soil. DNA is separated from soil contaminants by electrophoresis in low-melting-temperature agarose and used directly in polymerase chain reaction amplific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::897db83f9e4ac264282956f3c339bc45
https://doi.org/10.1007/bf01570868
https://doi.org/10.1007/bf01570868
Autor:
Michael V. Walter, L. Arlene Porteous, John L. Armstrong, Valerie P. Fieland, Ramon J. Seidler
Publikováno v:
Canadian Journal of Microbiology. 37:703-707
Conjugation on agar plates following mating trials can cause transconjugant numbers to be overestimated. Numbers of transconjugants detected after incubating donors and recipients together were compared with those observed when donors and recipients
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::993e78e193ce623038b078b1857e209e
https://doi.org/10.1139/m91-119
https://doi.org/10.1139/m91-119
Autor:
L. Arlene Porteous, John L. Armstrong
Publikováno v:
Current Microbiology. 22:345-348
We describe an extraction method that yields up to 25 µg of DNA from 1 gram of soil. Samples are processed within 48 h. The purified DNA is 20–25 kb in size and can be digested by restriction enzymes. The soil is vortex-mixed with 6 mL mixing buff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::46675650229c68fec57265161a867094
https://doi.org/10.1007/bf02092152
https://doi.org/10.1007/bf02092152
Publikováno v:
Agriculture, Ecosystems & Environment. 28:431-435
DNAs were extracted from two saprophytic and three mycorrhizal genera and digested with four restriction nucleases. The DNA fragments of varying lengths were separated by gel electrophoresis into patterns called restriction fragment length polymorphi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::45c3b054787216bea0ac2974d4244a14
https://doi.org/10.1016/0167-8809(90)90075-o
https://doi.org/10.1016/0167-8809(90)90075-o
Publikováno v:
Current microbiology. 29(5)
A rapid direct-extraction method was used to obtain DNA from environmental soil samples. Heat, enzymes, and guanidine isothiocyanate were utilized to lyse cells. The DNA was purified by agarose gel electrophoresis, amplified with 16S rRNA-based prime
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdcc27740f85cb90a7a614c6fe120d2c
https://pubmed.ncbi.nlm.nih.gov/7765420
https://pubmed.ncbi.nlm.nih.gov/7765420
Autor:
Paul T. Rygiewicz, John L. Armstrong
Publisher Summary The purpose of this chapter is to present a primer of basic methods that have direct application to recovery of DNA from mycorrhizal fungi in soils. The methods are organized into three broad categories: cell lysis, DNA purification
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c4a0e38e3148144923f34a0eaba8eaa4
https://doi.org/10.1016/s0580-9517(08)70179-x
https://doi.org/10.1016/s0580-9517(08)70179-x
Publikováno v:
Applied and environmental microbiology. 56(5)
Transconjugants arising from transfer of plasmid R388::Tn 1721 between donor and recipient strains of Enterobacter cloacae were detected in samples from the digestive tracts and fecal pellets of variegated cutworms ( Peridroma saucia ).
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c384f05947c86441eb8f7a73dc989fc0
https://pubmed.ncbi.nlm.nih.gov/16348197
https://pubmed.ncbi.nlm.nih.gov/16348197
Publikováno v:
Applied and Environmental Microbiology. 55:2200-2205
Variegated cutworms were exposed to bean plants in microcosms sprayed with pBR322-carrying strains of Enterobacter cloacae, Klebsiella planticola, and Erwinia herbicola. The three bacterial species exhibited differential survival on leaves, in soil,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::744266d227cd6affa771ad7507938e4c
https://doi.org/10.1128/aem.55.9.2200-2205.1989
https://doi.org/10.1128/aem.55.9.2200-2205.1989
Publikováno v:
Current Microbiology. 15:229-232
A microcosm method was developed to investigate survial and fate of genetically engineered bacteria associated with plant surfaces and a plant-feeding insect, the variegated cutworm,Peridroma saucia. Larvae on radish plants in microcosms were sprayed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b9691479bf0125e9647d49d9e5794215
https://doi.org/10.1007/bf01577536
https://doi.org/10.1007/bf01577536
Publikováno v:
Applied and Environmental Microbiology. 54:343-347
A computer simulation model was used to predict the dynamics of survival and conjugation of Pseudomonas cepacia (carrying the transmissible recombinant plasmid R388:Tn1721) with a nonrecombinant recipient strain in simple rhizosphere and phyllosphere
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9eb2b539d1061b7947e2bcedb8c7b598
https://doi.org/10.1128/aem.54.2.343-347.1988
https://doi.org/10.1128/aem.54.2.343-347.1988